Reagent box used for separating bone marrow single nuclear cell

Abstract

This invention discloses a kit used for separating marrow mononuclear cell. This kit includes dilution, eluant, separating medium which consist of separating medium A and separating medium B; the stated dilution is isotonic Na chloride, PBS or Hank's liquid; eluant is isotonic Na chloride, PBS or Hank's liquid; separating medium A is hydroxyethyl starch solution whose quality / volume percentage concentration is 5.5-6.5% or cellogran solution whose quality / volume percentage concentration is 0.45-0.55%; Separating medium B is ficoll- hypaque solution (trade name is ficoll solution) whose density is 1.077g / mL or Percoll liquid whose density is 1.073g / mL.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for isolating bone marrow mononuclear cells. Background technique [0002] Limb ischemic diseases caused by arterial obstruction (arteriosclerotic obliterans of the lower extremities, diabetic limb ischemia, thromboangiitis obliterans, etc.) are common and refractory diseases in my country and even in the world. With the increase of the elderly population, the number of cases is gradually increasing. The risk factors associated with its pathogenesis include hypertension, hyperlipidemia, diabetes, smoking, lack of exercise, and population aging, etc. These risk factors are developing in a direction that is not conducive to disease control. According to reports from western countries, the incidence rate of this type of disease is as high as 15% among adults over 55 years old, and more than 1 / 3 of them have typical intermittent claudication symptoms; 12.1%. In the advanced stage of this disease, the...

AI Technical Summary

Problems solved by technology

However, there is currently no kit for isolating bone marrow mononuclear cells, making the progress of related research work relatively slow