Loc device with dialysis section for separating leukocytes from blood

a technology of leukocytes and dialysis sections, applied in the field of diagnostic devices, can solve the problems of slow growth of this type of testing in the clinical laboratory, reduced sensitivity, and high degree of non-specific binding, and achieve the effects of simple manufacturing procedures, low system component count, and increased sensitivity

Inactive Publication Date: 2011-12-22
GENEASYS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0090]The easily usable, mass-producible, and inexpensive LOC device accepts a blood sample, uses a dialysis section for separating the leukocytes, and separately processes the nucleic acid content of the leukocytes separated from the blood sample.
[0091]The dialysis section functionality extracts add...

Problems solved by technology

Insufficient stringency can result in a high degree of nonspecific binding.
Excessive stringency can lead to a failure of appropriate binding, which results in diminished sensitivity.
Despite the advantages that molecular diagnostic tests offer, the growth of this type of testing in the clinical laboratory has been slower than expected and remains a minor part of the practice of laboratory medicine.
This is primarily due to the complexity and costs associated with nucleic acid testing compared with tests ba...

Method used

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  • Loc device with dialysis section for separating leukocytes from blood
  • Loc device with dialysis section for separating leukocytes from blood
  • Loc device with dialysis section for separating leukocytes from blood

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Embodiment Construction

Overview

[0226]This overview identifies the main components of a molecular diagnostic system that incorporates embodiments of the present invention. Comprehensive details of the system architecture and operation are set out later in the specification.

[0227]Referring to FIGS. 1, 2, 3, 130 and 131, the system has the following top level components:

[0228]Test modules 10 and 11 are the size of a typical USB memory key and very cheap to produce. Test modules 10 and 11 each contain a microfluidic device, typically in the form of a lab-on-a-chip (LOC) device 30 preloaded with reagents and typically more than 1000 probes for the molecular diagnostic assay (see FIGS. 1 and 130). Test module 10 schematically shown in FIG. 1 uses a fluorescence-based detection technique to identify target molecules, while test module 11 in FIG. 130 uses an electrochemiluminescence-based detection technique. The LOC device 30 has an integrated photosensor 44 for fluorescence or electrochemiluminescence detection...

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Abstract

A lab-on-a-chip (LOC) device to for analyzing leukocytes in a blood sample, the LOC device having a supporting substrate, an inlet for receiving a sample of blood including leukocytes and erythrocytes, a dialysis section for separating at least some of the erythrocytes from the leukocytes, and, a microsystems technology (MST) layer for separately analyzing the leukocytes, wherein, the inlet, the dialysis section and the MST layer are all supported on the supporting substrate.

Description

FIELD OF THE INVENTION[0001]The present invention relates to diagnostic devices that use microsystems technologies (MST). In particular, the invention relates to microfluidic and biochemical processing and analysis for molecular diagnostics.CO-PENDING APPLICATIONS[0002]The following applications have been filed by the Applicant which relate to the present application:GBS001USGBS002USGBS003USGBS005USGBS006USGSR001USGSR002USGAS001USGAS002USGAS003USGAS004USGAS006USGAS007USGAS008USGAS009USGAS010USGAS012USGAS013USGAS014USGAS015USGAS016USGAS017USGAS018USGAS019USGAS020USGAS021USGAS022USGAS023USGAS024USGAS025USGAS026USGAS027USGAS028USGAS030USGAS031USGAS032USGAS033USGAS034USGAS035USGAS036USGAS037USGAS038USGAS039USGAS040USGAS041USGAS042USGAS043USGAS044USGAS045USGAS046USGAS047USGAS048USGAS049USGAS050USGAS054USGAS055USGAS056USGAS057USGAS058USGAS059USGAS060USGAS061USGAS062USGAS063USGAS065USGAS066USGAS067USGAS068USGAS069USGAS070USGAS080USGAS081USGAS082USGAS083USGAS084USGAS085USGAS086USGAS087USGAS...

Claims

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Application Information

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IPC IPC(8): C40B60/12C12M1/34
CPCB01L3/5027Y10T436/25B01L3/502738B01L7/52B01L2200/10B01L2300/023B01L2300/024B01L2300/0636B01L2300/0654B01L2300/0883B01L2300/10B01L2300/1827B01L2400/0406B01L2400/0633B01L2400/0677B01L2400/0688F16K99/003F16K99/0036G01N27/223C12Q1/68Y10T436/107497Y10T436/173845Y10T436/143333Y10T436/11Y10T436/145555Y10T436/203332Y10T436/25375B01L3/502707Y10T137/0352Y10T137/0391Y10T137/1044Y10T137/206Y10T137/2076Y10T137/2202Y02A90/10
Inventor AZIMI, MEHDISILVERBOOK, KIA
Owner GENEASYS
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