33results about How to "Low system component-count" patented technology

A lab-on-a-chip (LOC) device for pathogen detection, genetic analysis and proteomic analysis of a biological sample, using a leukocyte dialysis section for separating out a leukocyte stream, a pathogen dialysis section for separating out a pathogen stream. A leukocyte lysis section lyses the leukocytes and a leukocyte incubation section allows enzymatic reaction of the genetic material with enzymes. A pathogen lysis section lyses the pathogens and, a pathogen incubation section allows enzymatic reaction of the genetic material with enzymes. An erythrocyte lysis section lyses the erythrocytes. First and second leukocyte nucleic acid amplification sections amplify nucleic acid sequences in parallel. First and second pathogen nucleic acid amplification sections amplify nucleic acid sequences in parallel.

A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the cells in the lysis section, a first nucleic acid amplification section downstream of the lysis section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate.

A lab-on-a-chip (LOC) device for pathogen detection, genetic analysis and proteomic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a leukocyte dialysis section for dividing the sample into a leukocyte stream, and a pathogen and erythrocyte stream containing cells and sample constituents smaller than a first size threshold, a pathogen dialysis section for dividing the pathogen and erythrocyte stream into an erythrocyte stream and a pathogen stream containing cells and sample constituents smaller than a second size threshold, a leukocyte lysis section downstream of the leukocyte dialysis section for lysing the leukocytes with a lysis reagent to release genetic material and proteins therein, a leukocyte incubation section downstream of the leukocyte lysis section for enzymatic reaction of the genetic material with enzymes, a pathogen lysis section downstream of the pathogen dialysis section for lysing the pathogens with a lysis reagent to release genetic material and proteins therein, a pathogen incubation section downstream of the pathogen lysis section for enzymatic reaction of the genetic material with enzymes, an erythrocyte lysis section downstream of the pathogen dialysis section for lysing the erythrocytes with a lysis reagent to release proteins therein, a first leukocyte nucleic acid amplification section downstream of the leukocyte incubation section for amplifying nucleic acid sequences, a second leukocyte nucleic acid amplification section downstream of the first leukocyte nucleic acid amplification section for amplifying nucleic acid sequences, a first pathogen nucleic acid amplification section downstream of the pathogen incubation section for amplifying nucleic acid sequences, and, a second pathogen nucleic acid amplification section downstream of the first pathogen nucleic acid amplification for amplifying nucleic acid sequences, wherein, the leukocyte dialysis section, the pathogen dialysis section, the leukocyte lysis section, the pathogen lysis section, the leukocyte incubation section, the pathogen incubation section, the first and second leukocyte nucleic acid amplification sections and the first and second pathogen nucleic acid amplification sections are all supported on the supporting substrate.

A microfluidic device for detecting target molecules in a fluid, the microfluidic device having an array of chambers containing electrochemiluminescent (ECL) probes for reaction with the target molecules to form a probe-target complexes, electrodes positioned in each of the chambers for receiving an electrical pulse, the probe-target complexes being configured to emit a photon of light when excited by current between the electrodes, and, a photosensor for detecting the light emitted by the probes, wherein, the photosensor is less than 1600 microns from the probes.

A lab-on-a-chip (LOC) device to separate pathogens and leukocytes from a blood sample for analysis, the LOC device having a sample inlet for receiving the blood including pathogens, leukocytes and other constituents, a dialysis section for separating the pathogens into a first portion of the sample and separating the leukocytes into a second portion of the sample such that the remainder of the sample has constituents smaller than the leukocytes and larger than the pathogens, and, a microsystems technology (MST) layer for analyzing the pathogens and the leukocytes.

A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample containing genetic material including DNA and RNA, a supporting substrate, a plurality of reagent reservoirs, a first incubation section, the first incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a second incubation section, the second incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material in parallel with the first incubation section, a first nucleic acid amplification section downstream of the first incubation section for amplifying at least some of the genetic material, and, a second nucleic acid amplification section downstream of the second incubation section for amplifying at least some of the genetic material in parallel with the first nucleic acid amplification section, wherein, the first incubation section, the second incubation section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate.

A test module for detecting hybridization of probes with target nucleic acid sequences, the test module having a receptacle for receiving a biological sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, an excitation light emitting diode (LED) for illuminating the array of probes, CMOS circuitry having a photosensor for sensing a fluorescence emission from any of the probes in the array that have hybridized with the target nucleic acid sequences such that the CMOS circuitry is configured to use outputs from the photosensor to generate a signal indicative of the probes that have hybridized, wherein, the array of probes and the CMOS circuitry are integrated on a lab-on-a-chip (LOC) device contained within the test module.

A lab-on-a-chip (LOC) device for pathogen detection and genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a lysis section for lysing pathogens and leukocytes in the sample to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the pathogens and leukocytes in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a first nucleic acid amplification section downstream of the incubation section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the lysis section, the incubation section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate.

A lab-on-a-chip (LOC) device for pathogen detection and genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a lysis section for lysing pathogens and leukocytes in the sample to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the pathogens and leukocytes in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, and, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences from the genetic material, wherein, the lysis section, the incubation section and the nucleic acid amplification section are all supported on the supporting substrate.

A test module for amplifying nucleic acid sequences, the test module having an outer casing with receptacle for receiving a sample containing genetic material, a plurality of reagent reservoirs containing reagents for addition to the sample, a first nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material in parallel with the first nucleic acid amplification section.

A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample containing genetic material, a supporting substrate, a plurality of reagent reservoirs, a first incubation section, the first incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a second incubation section, the second incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material in parallel with the first incubation section, a first nucleic acid amplification section downstream of the first incubation section for amplifying nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the second incubation section for amplifying nucleic acid sequences in the genetic material in parallel with the first nucleic acid amplification section, wherein, the first incubation section, the second incubation section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate.

A microfluidic device for processing a fluid sample, the microfluidic device having a reservoir for containing a reagent, and, a surface tension valve having an aperture configured to pin a meniscus of the reagent such that the meniscus retains the reagent in the reagent reservoir until contact with the fluid sample removes the meniscus such that the reagent flows out of the reagent reservoir.

A microfluidic device having a supporting substrate, a hybridization chamber containing probes having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form probe-target hybrids, the probe-target hybrids being configured to generate a fluorescence signal in response to an excitation light, and, a photodiode with an active area and an optical axis extending normal to the active area and through the hybridization chamber, wherein, the active area is less than 249 microns from the probe-target hybrids.

A microfluidic device for testing a fluid, the microfluidic device having an inlet for receiving the fluid, a reservoir containing a reagent, a flow-path extending from the inlet, a plurality of outlet valves for fluid communication between the flow-path and the reservoir, each of the outlet valves having an actuator for opening the outlet valve in response to an activation signal, wherein during use, a number of the outlet valves are selectively opened such that the reagent flows into the flow-path to combine with the fluid from the inlet to produce a combined flow having a proportion of the reagent, the proportion of the reagent in the combined flow being determined by the number of the outlet valves opened.

A method of analyzing the nucleic acid content of a biological fluid, the method comprising the steps of: providing a test module with an outer casing configured for handheld portability, the outer casing having a receptacle for receiving the biological fluid, the test module having a hybridization section with an array of probes for hybridization with target nucleic acid sequences in the biological fluid, and circuitry for sensing which of the probes have hybridized and generating hybridization data, providing a test module reader for reading the hybridization data from the test module, inserting the biological fluid in the receptacle, interfacing the test module with the test module reader, wherein, the test module reader analyses the nucleic acid content from the hybridization data.

A microfluidic device for lysing cells in a fluid, the microfluidic device having a supporting substrate, an inlet for receiving fluid containing cells, a lysis section in fluid communication with the inlet, the lysis section having at least one heater for heating the fluid, a reservoir containing a lysis reagent, and, CMOS circuitry between the supporting substrate and the lysis section, the CMOS circuitry being configured for selectively lysing the cells using thermal lysis in the lysis section, chemically lysing the cells using the lysis reagent, or both chemically and thermally lysing the cells using the lysis section and the lysis reagent.

A microfluidic device for lysing cells in a fluid, the microfluidic device having an inlet channel for receiving a fluid containing cells, a lysis section in fluid communication with the inlet channel for holding the fluid during lysis of the cells, and, a lysis heater for heating the fluid in the lysis section such that the cells lyse, wherein, the inlet channel is configured for filling the lysis section by capillary action.

A microfluidic device for processing a fluid sample, the microfluidic device having a first channel configured to fill with the sample by capillary action, a second channel configured to fill with the sample by capillary action, a plurality of fluid connections between the first channel and the second channel, one of the fluid connections having an active valve to arrest the sample flow into the second channel and the remaining fluid connections each being configured to pin a meniscus of the sample that arrests capillary flow between the first channel and the second channel, the fluid connection with the active valve being upstream of the remaining fluid connections, wherein during use, the sample flow into the second channel is initiated by activation of the active valve such that the sample flow in the second channel progressively removes the meniscus at each of the meniscus anchors.

A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the target cells to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the target cells in the lysis section, a first nucleic acid amplification section downstream of the lysis section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate.

A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving a biological sample containing target nucleic acid sequences, probes for hybridization with the target nucleic acid sequence to form probe-target hybrids, and, a plurality of reagent reservoirs containing all reagents necessary for performing the diagnostic assay.

A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the cells in the lysis section, and, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material, wherein, the dialysis section, the lysis section and the nucleic acid amplification section are all supported on the supporting substrate.

A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the target cells to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the target cells in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, and, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences from the genetic material, wherein, the dialysis section, the lysis section, the incubation section and the nucleic acid amplification section are all supported on the supporting substrate.

A microfluidic device having a supporting substrate, a hybridization chamber containing probes having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form probe-target hybrids and generate a fluorescence signal in response to an excitation light, and, a photodiode with an active area and an optical axis extending normal to the active area and through the hybridization chamber, wherein, the hybridization chamber has a floor surface positioned parallel to the active area of the photodiode, the floor surface having a centroid and the active area being encompassed within a cone having the centroid at its vertex, and a vertex angle less than 173°.

A microfluidic device for amplifying nucleic acid sequences, the microfluidic device having an inlet for receiving a sample containing genetic material, a plurality of reagent reservoirs containing reagents for addition to the sample, and, a nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material.

A test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing having a receptacle for receiving the fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, and, a detection photosensor for exposure to the photons emitted by the ECL luminophores.

A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section having a lysis chamber and a heater for lysing the pathogens while the sample is in the lysis chamber, a first nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material in a first portion of the sample flow from the lysis section, and, a second nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material in a second portion of the sample flow from the lysis section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate.

A test module for analyzing fluid containing target nucleic acid sequences, the test module having a casing for hand-held portability, the casing having a receptacle for receiving the fluid, an array of probes for hybridization with target nucleic acid sequences to form probe-target hybrids, and, a waste accumulator for storage of waste material separated from the fluid, wherein, the waste accumulator has a waste reservoir and a porous element to draw the waste material into the waste reservoir by capillary action.

A LOC device for performing a genetic diagnostic assay, the LOC device having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving a biological sample containing target nucleic acid sequences, probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, a flow-path extending from the inlet to the probes, and, a reagent reservoir containing a reagent for addition to the sample in the flow-path upstream of the probes.

A lab-on-a-chip (LOC) device for analyzing leukocytes in a blood sample, the LOC device having a supporting substrate, an inlet for receiving a sample of blood, a dialysis section for separating erythrocytes from leukocytes in the blood, and, a microsystems technology (MST) layer for analyzing the leukocytes, wherein, the inlet, the dialysis section and the MST layer are all supported on the supporting substrate.

A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer on the supporting substrate for analyzing a fluid, and, a waste reservoir for storage of waste material separated from the fluid.