Methods and compositions for improved cattle longevity and milk production
a technology for milk production and cattle longevity, applied in the field of cattle progeny testing, can solve the problems of severe gh deficiency, time-consuming and expensive standard technique, and selection based purely on phenotypic characteristics cannot efficiently take into account genetic variability,
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example 1
Identification of SNP
[0069]Sequencing of 30 grandsires from the CDDR populations and of the pooled DNA samples revealed four SNP identified in protein coding exons of POU1F1. An A / G SNP at position 3699 (GenBank accession number NW—001501776) was identified in exon 2, and 3 SNP were identified in exon 3: SNP A / C at position 10793, SNP C / T at position 10822, and SNP A / G at position 10863. Importantly, SNP3699 (exon 2), SNP10822 (exon 3), and SNP10863 (exon 3) were found to be in complete linkage disequilibrium (LD), therefore only SNP3699 was used for genotyping. SNP10793 (exon 3) was not in LD with other SNPs, therefore it was genotyped independently. SNP3699, SNP10822, and SNP10863 are synonymous mutations whereas SNP10793 is a missense mutation in which the change from a C to an A (minor allele) changes amino acid 76 of the POU1F1 protein from proline (Pro) to histidine (His). Alignment of protein sequences of POU1F1 from mouse, rat, human, chimpanzee, bovine, and dog using the mu...
example 2
Association of POU1F1 with Milk Production and Health Traits
[0070]The allele and genotype frequencies of SNP3699 and SNP10793 and the corresponding chi square test of Hardy-Weinberg equilibrium (HWE) are listed in Table 1. The genotype frequencies were consistent with those expected of a population in Hardy-Weinberg equilibrium. Association testing of SNP3699 in the CDDR population did not show significance with any of the examined traits (data not shown), so this SNP was not investigated in the UW resource population.
[0071]In contrast to SNP3699, SNP10793 was found to be significantly associated (P=0.027) with milk yield in the CDDR population using the allele substitution model (Table 2). PTAs analysis in the UW population did not detect association of milk yield with POU1F1 locus. However, in the YD analysis, AA genotype was found to be associated with higher milk yield (Table 3). The AA genotype was also found to be positively associated with productive life. Because of PTAs add...
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