3 results about "Multiple birth" patented technology

The application provides a primer pair for detecting a multiple birth trait molecular marker of Mongolian sheep, and a detection method and application thereof, and belongs to the technical field of molecular marker assisted breeding. The primer pair for detecting the multiple birth trait molecular marker of the Mongolian sheep comprises primer pair 1 or primer pair 2. The primer pair 1 can detect a polymorphic site at the 7828531th position of exon 6 of an MSI2 gene, the nucleotide of the site is A or G, and a Mongolian sheep with a genotype AA has a multiple birth trait. The primer pair 2 can detect a polymorphic site at the 8144512th position of exon 11 of the MSI2 gene, the nucleotide of the site is C or G, and a Mongolian sheep with a genotype CC has a multiple birth trait. In the process of breeding and cultivating the Mongolian sheep, the AA type or the CC type individual with a twin birth trait can be selected through the SNP molecular marker assisted selection, so that the breeding process of the reproductive performance of the Mongolian sheep is accelerated.

The invention relates to the technical field of molecular biology, in particular to a detection method of a sheep multi-fetal gene FecB genotype and an identification method of sheep multi-fetal traits. The specific LAMP primer pair of the sheep multi-fetal gene FecB is designed, the detection method of the genotype of the sheep multi-fetal gene FecB is established based on the primer pair, large-scale detection of single nucleotide polymorphic sites of the FecB gene can be realized, and the sheep multi-fetal traits can be identified according to the detection result. The method has the advantages of simplicity in operation, high efficiency, rapidness and high sensitivity, provides an efficient and convenient detection method for early selection and molecular breeding of sheep, and has a wide application prospect.

The invention relates to the technical field of molecular biology, in particular to a method for detecting the genotype of a sheep multi-fetal major gene FecB based on an isothermal amplification technology. The invention provides the specific RPA primer group for the sheep multi-fetal major gene FecB, and a novel method for typing the polymorphic sites of the sheep multi-fetal major gene FecB is established by utilizing an RPA technology, and the method has the advantages of simplicity in operation, high efficiency, rapidness and high accuracy; genetic typing of the sheep multi-fetal major gene FecB and identification of sheep multi-fetal character individuals can be rapidly achieved on a large scale, a pasture is helped to conduct early selection on high-reproduction breeding stock, the group reproduction efficiency can be effectively improved, genetic improvement of the lambing number, the lambing rate and other characters of a sheep flock can be accelerated, continuous optimization of the population is achieved, and the method has wide application prospects.