5 results about "Multiple birth" patented technology

The application provides application of YTHDC1 gene as a detection target and application of a SNP molecular marker related to a single-twin multiple-lamb trait of sheep, and belongs to the technical field of molecular markers and genetic breeding. The application provides application of YTHDC1 gene as a detection target. The lambing performance of a to-be-tested sheep can be determined by rapidly typing the YTHDC1 genotype of a newborn sheep, and the breeding of a ewe is facilitated. The application also provides application of a SNP molecular marker related to a single-twin multiple-lamb trait of sheep. Based on sheep genome sequence information (Oar_v3.1), the SNP molecular marker is located at a 84064404bp site on a 6th chromosome of sheep, and A / G polymorphism exists. The SNP molecular marker is significantly related to the single-twin high-lamb-number trait of sheep, and in the production process of sheep, individuals with the GG type of the polymorphic site can be selected and bred, so that the reproductive capacity of a sheep population is improved.

The application provides a primer pair for detecting a multiple birth trait molecular marker of Mongolian sheep, and a detection method and application thereof, and belongs to the technical field of molecular marker assisted breeding. The primer pair for detecting the multiple birth trait molecular marker of the Mongolian sheep comprises primer pair 1 or primer pair 2. The primer pair 1 can detect a polymorphic site at the 7828531th position of exon 6 of an MSI2 gene, the nucleotide of the site is A or G, and a Mongolian sheep with a genotype AA has a multiple birth trait. The primer pair 2 can detect a polymorphic site at the 8144512th position of exon 11 of the MSI2 gene, the nucleotide of the site is C or G, and a Mongolian sheep with a genotype CC has a multiple birth trait. In the process of breeding and cultivating the Mongolian sheep, the AA type or the CC type individual with a twin birth trait can be selected through the SNP molecular marker assisted selection, so that the breeding process of the reproductive performance of the Mongolian sheep is accelerated.

The application belongs to the field of molecular biology, and particularly relates to a specific primer of a molecular marker of a Mongolian sheep gene B4GALNT2 and application thereof. The specific primer of the molecular marker of the Mongolian sheep gene B4GALNT2 is shown in SEQ ID NO. 1 and SEQ ID NO. 2. The specific primer is designed to detect whether a mutation of a g.25929793G / T site exists at a region from 25929578 bp to 25930324 bp of a 7th intron of the B4GALNT2 gene in a genome of the Mongolian sheep, so as to compare polymorphism of the B4GALNT2 gene in the Mongolian sheep breed. The application utilizes polymorphism of the B4GALNT2 gene at the g.25929793G / T site to assist in breeding of the Mongolian sheep, and improve lambing number of the Mongolian sheep, and can be used as an effective method for assisting in improving a multiple birth trait of the Mongolian sheep.

The invention relates to the technical field of molecular biology, in particular to a detection method of a sheep multi-fetal gene FecB genotype and an identification method of sheep multi-fetal traits. The specific LAMP primer pair of the sheep multi-fetal gene FecB is designed, the detection method of the genotype of the sheep multi-fetal gene FecB is established based on the primer pair, large-scale detection of single nucleotide polymorphic sites of the FecB gene can be realized, and the sheep multi-fetal traits can be identified according to the detection result. The method has the advantages of simplicity in operation, high efficiency, rapidness and high sensitivity, provides an efficient and convenient detection method for early selection and molecular breeding of sheep, and has a wide application prospect.

The invention relates to the technical field of molecular biology, in particular to a method for detecting the genotype of a sheep multi-fetal major gene FecB based on an isothermal amplification technology. The invention provides the specific RPA primer group for the sheep multi-fetal major gene FecB, and a novel method for typing the polymorphic sites of the sheep multi-fetal major gene FecB is established by utilizing an RPA technology, and the method has the advantages of simplicity in operation, high efficiency, rapidness and high accuracy; genetic typing of the sheep multi-fetal major gene FecB and identification of sheep multi-fetal character individuals can be rapidly achieved on a large scale, a pasture is helped to conduct early selection on high-reproduction breeding stock, the group reproduction efficiency can be effectively improved, genetic improvement of the lambing number, the lambing rate and other characters of a sheep flock can be accelerated, continuous optimization of the population is achieved, and the method has wide application prospects.