The utility model relates to the technical field of fruit packaging, in particular to an anti-collision fruit fresh-keeping packaging box which comprises a box body, a box cover is rotatably connected to the rear portion of the top of the box body, exhaust holes are formed in the left side and the right side in the box body, and a damping spring piece is fixedly connected to the lower portion in the box body. Guide grooves are formed in the front, back, left, right and middle of the inner wall of the box body, and guide rails are slidably connected to the inner walls of the guide grooves; through design cooperation of damping spring pieces and small spring pieces, vibration generated when the bottom of a box body makes contact with the ground and affecting vibration of fruits in a containing groove can be reduced, bottom sponges are arranged at the bottom of the containing groove of a containing disc, edge sponges are arranged on the inner sides of clamping rings, and the bottoms and the edges of the fruits can be protected at the same time; the situation that the surfaces of the fruits are damaged under the condition that the placing disc made of hard materials shakes is avoided, the stability of the fruits during transportation is improved to a certain degree, and the integrity of the fruits in the box body is guaranteed.