Quick-speed PCR quantitative determination method of sulfate-reducing bacteria direct dilution by multiple proportions

Abstract

This invention discloses to a sulfate reducing bacterium timely diluting PCR rapid quantitative determination method, which relates to the sulfate reducing bacterium (SRB) quantitative determination method. This invention comprises the following steps: (1) preparing 'thalli formal dispose cushion succus'; (2)preparing the thalli;(3)timely diluting; (4) making the PCR operation on the ice; (5) PCR expanding on the expanding device; (6) 1.0% agarose tiselius detectiong the expanded production;(7) Observing result, recording number. The result of this method is accurate, which can shorten the account time effectively and reflect the physical production condition actually.

Description

technical field

[0001] The invention relates to a rapid and quantitative detection method for sulfate-reducing bacteria (SRB) in the water treatment process of conventional sewage, poly-containing sewage and surface technology in an oil field water system. Background technique

[0002] At present, the counting of SRB bacteria in the oilfield system mainly follows the Chinese oil and gas industry standard, "Bacterial Analysis Method for Oilfield Injection Water - Extinct Dilution Method (MPN)" (Ministerial Standard), SY-T0532-93. The problem with the above-mentioned method is that the detection takes 14 days. Due to the long detection cycle, it cannot effectively and immediately guide the production practice. In the actual operation process, some strict anaerobic SRB bacteria cannot survive, resulting in SRB The counting result of bacteria is inaccurate, cannot truly reflect the actual production situation, and the detection cost is high; and because the existing detection me...

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