Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting bacterial AmpC enzyme using paper disk containing 3-amino phenol borate

An aminophenol and paper technology, which is applied to the detection field of biological bacteria, can solve the problems of increasing operation steps, lack of methods, and complicated operation, and achieves the effects of good repeatability, low cost, and improved positive rate.

Inactive Publication Date:
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no simple and effective method for routine detection of AmpC-producing bacteria in clinical laboratories
Three-dimensional assay to detect AmpC enzyme (Coudron PE, Moland ES, Thomson KS. Occurrence and detection of AmpC beta-lactamases among Escherichia coli, Klebsiella pneumoniae, and proteus mirabilis isolates at a veterans medical center. J ClinMicrobiol, 2000, 38: 1791-1796) , it is necessary to prepare the crude extract of the enzyme and dig grooves on the agar plate, the operation is cumbersome, time-consuming, and requires special equipment such as ultrasonic breaker or repeated freezing and thawing conditions
The latest method for detecting AmpC enzyme with Tris-EDTA introduced abroad (Black JA, Moland ES, and Thomson KS. AmpCdisk test for detection of plasmamid-mediated AmpC β-lactamases in Enterobacteriaceae lacking chromosome AmpC β-lactamases. J ClinMicrobiol, 2005, 43: 3110-3113) Although it has the characteristics of simple operation and low cost, it needs to prepare paper containing Tris-EDTA and the bacteria to be tested, which increases the operation steps, and the interpretation of the results of some bacteria requires certain experience

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] The invention provides a method for detecting bacterial AmpC enzyme with a paper sheet containing 3-aminophenol boronic acid. The detection principle of this method is that because boric acid is an inhibitor of AmpC enzyme, this invention adds APB to CAZ, CTX and FOX paper on the basis of CAZ without 3-aminophenol boronic acid APB, CTX and FOX paper. Disc susceptibility test, that is, APB disc enhanced method, detects the AmpC enzyme produced by bacteria. APB can expand the inhibition zone of CAZ or / and CTX or / and FOX discs to AmpC enzyme-producing bacteria, when CAZ or / and CTX or / and FOX discs containing APB are more than CAZ or / and CTX without APB Or / and when the diameter of the FOX disc antibacterial ring increases by 5 mm or more, it is judged that the AmpC enzyme test is positive.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

This invention discloses a method for testing bacteria AmpC enzyme with paper containing 3-amino-phenol-boric acid. It prepares APB solution first and adds APB on the CAZ, CTX and FOX paper without APB to apply APB paper reinforcement method to carry out paper method drug hypersensitive test to test the AmpC enzyme generated by bacteria. APB can enlarge the microbiostatic ring of CAZ, CTX or FOX paper to the generated AmpC enzyme bacteria so as to judge the existence of AmpC enzyme by testing the change of the diameter of the ring to avoid missed test brought with using the single base material to increase the positive rate.

Description

technical field [0001] The invention belongs to the technical scope of biological bacteria detection, in particular to a method for detecting bacterial AmpC enzyme with a paper sheet containing 3-aminophenol boric acid (APB). Background technique [0002] AmpCβ-lactamases, which belong to the class C enzymes classified by Ambler (hereinafter referred to as AmpC enzymes), are produced by some bacteria such as Klebsiella pneumoniae and Escherichia coli during the growth process and can hydrolyze third-generation cephalosporin antibiotics (such as ceftazidime) CAZ, cefotaxime CTX, ceftriaxone CRO), cephamycin (such as cefoxitin FOX), monoamine antibiotics (such as aztreonam AZT) enzymes. Bacteria that produce AmpC enzyme are resistant to the above antibiotics, and when these bacteria cause infections such as sepsis, pneumonia, pyelonephritis or wound infection, the use of the above antibiotics will make the treatment fail. Therefore, the correct detection of bacteria producing...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/25C12Q1/04
Inventor 沈定霞罗燕萍张有江徐雅萍周光
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com