Laser induction fluorescence detector

Abstract

The invention discloses a laser induction fluorescence detecting machine, which is mainly used for the micro sample inspecting in the field of clinical medicine and life science. The invention comprises an excitation light path (10), a separating micro column (20) and a fluorescence light collecting path (30). The axis shaft line of the excitation light path (10) is vertical with the axis shaft line of the fluorescence light collecting path (30), and the excitation light path (10) has the same axis with the separating micro column (20), an optical fiber (40) is equipped between the excitation light path (10) and the separating micro column (20); the front end of the optical fiber (40) is coupled with the excitation light path (10), and the rear end extends to the inspecting window (21) of the separating micro column (20). The invention has the advantage that the invention has high fluorescence stimulation efficiency, The optical structure is simple, the volume is small, the fluorescence stimulation of the sample can be directly realized, the sensitivity and accuracy can be enhanced; and the invention can be used together with the micro column separating systems like capillary tube electrophoresis, capillary tube liquid chromatography and flowing injection, etc.

Description

technical field [0001] The invention relates to a detection and analysis instrument, in particular to an in-column laser-induced fluorescence detector which is mainly suitable for the detection of trace samples in the fields of clinical medicine and life science. Background technique [0002] In recent years, in order to adapt to the development trend of miniaturization of analytical instruments, various micro-column separation methods have emerged as the times require. High-sensitivity detection methods have always been an important content of analytical chemistry research, so improving the sensitivity of detectors has become a key issue in the practical application of micro-column separation technology. Since the inner diameter of the microcolumn is very small (generally tens to hundreds of microns), the volume of the detection window on the column is only a few to several hundred nanoliters, and the conventional ultraviolet photometric detection method cannot meet the hig...

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Problems solved by technology

However, in these two optical structures, the incident light must pass through the wall of the microcolumn (capillary) to excite the sample, which is bound to produce a certain amount of light reflection and scattering on the capillary wall, so that the light emitted by the laser cannot be fully used to excite the fluorescence. This will lead to higher background noise, affect the fluorescence excitation efficiency and detection sensitivity, and make it difficult to ensure or improve the detection efficiency of the micro-column separation system

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