Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing polynucleotides for analysis

A technology of polynucleotides and target polynucleotides, applied in biochemical equipment and methods, measurement/inspection of microorganisms, etc., can solve problems such as readout step obstruction

Inactive Publication Date: 2008-03-12
LINGVITAE AS
View PDF3 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] One difficulty with prior art methods is that the final readout step is often hampered by the need to distinguish between different amplified tags or cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing polynucleotides for analysis
  • Method for preparing polynucleotides for analysis
  • Method for preparing polynucleotides for analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0086] To illustrate the "rollback" principle, a 114nt single-stranded molecule was used as the second polynucleotide substrate and the 38bp circular target template. The second polynucleotide was "anchored" by immobilizing the substrate molecule with biotin on paramagnetic beads coated with 1 [mu]M streptavidin.

[0087] The target molecule is hybridized to the second polynucleotide and phi29 DNA polymerase is added. The polymerase performs an extension reaction using the target as a template. The extended strand is complementary to the second polynucleotide, and according to the rollback theory, the extended strand will hybridize to the second polynucleotide to form a 114bp double-stranded molecule. Depending on the sequence of the target, the double-stranded molecule will contain recognition sites for certain restriction endonucleases (Figures 10, 11 and 12).

[0088] As shown in FIG. 10 , the target having the unit sequence 0100 forms a recognition site for BamHI at bit ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method for analysing a target polynucleotide having distinct units of nucleic acid sequence comprising: (i) forming a first polynucleotide which is a concatemer having multiple repeating target polynucleotide sequences; (ii) forming on the first polynucleotide a second polynucleotide hybridised to a portion of one or more of the target polynucleotides, such that the portion hybridised, or the portion not hybridised, corresponds to a sequence unit on the target, and determining the sequence unit on the target.

Description

technical field [0001] The present invention relates to methods of modifying polynucleotides so that the polynucleotides can be more easily analyzed. Background technique [0002] Advances in molecular research have been driven in part by improvements in techniques used to characterize molecules or their biological responses. In particular, the study of the nucleic acids DNA and RNA has benefited from technological developments in sequence analysis and the study of hybridization reactions. [0003] WO-A-00 / 39333 describes a method for sequencing polynucleotides by converting the sequence of a target polynucleotide into a second polynucleotide having a defined sequence and location information. It is reported that the sequence information of the target is "amplified" in the second polynucleotide, making it easier to distinguish individual bases in the target molecule. This can be achieved through the use of "amplified tags" with predetermined nucleic acid sequence units. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6813C12Q2525/151C12Q2531/125C12Q1/6806
Inventor P·莱克索
Owner LINGVITAE AS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com