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Cabrite medicinal materials discrimination method

A technology for lizards and medicinal materials, applied in the field of medicinal material identification, can solve the problems of poor specificity and negative interference, and achieve the effect of good specificity and good identification effect.

Active Publication Date: 2008-07-02
山西华元医药生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Ministry of Health Drug Standards Chinese Medicine Prescriptions" (Volume 11) page 217 only contains the source of the lizard, and "Jiangu Xujin Tablets" [identification] (2) on page 170 of the book provides A test tube experiment for lizard identification, which is the ninhydrin reaction of proteins in lizards, but the test results of this method show negative interference and poor specificity
Until now, there is no specific chemical identification method for lizard medicinal materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The identification of lizards derived from the dry whole of Eremias argus Peters (hereinafter referred to as "Lipota") of Lizardidae.

[0027] Take 0.5g of Lischima lizard powder, add 20mL of ethanol, ultrasonic treatment for 20min, filter, evaporate the filtrate to dryness, add 1mL of methanol to dissolve the residue, and use it as the test solution. Take 0.5 g of the reference medicinal material of Lischimus lizard, and prepare a solution of the reference medicinal material in the same way.

[0028] Take 10 μL of each of the above two solutions, spot them on the same silica gel G thin-layer plate, use toluene: ethyl acetate = 1:1 as the developer, develop, take out, dry in the air, spray with 10% sulfuric acid ethanol solution, hot air Blow until the spots are clearly colored, and in the chromatogram of the test product, spots of the same color appear at the positions corresponding to the chromatogram of the reference medicinal material. Put the thin-layer plate unde...

Embodiment 2

[0030] Identification of lizard medicinal ingredients in Jieguxujin Capsules (capsules made according to the prescription of the Ministry of Health's drug standard WS3-B-2226-96).

[0031] Take 1 g of the content of Jieguxujin capsule, add 20 mL of ethanol, ultrasonically treat for 20 minutes, filter, evaporate the filtrate to dryness, add 1 mL of methanol to dissolve the residue, and use it as the test solution. Take 0.5 g of the lizard reference medicinal material, and prepare the reference medicinal material solution in the same way.

[0032] Take 10 μL of each of the above two solutions, spot them on the same silica gel G thin-layer plate, use toluene: ethyl acetate = 1:1 as the developer, develop, take out, dry in the air, spray with 10% sulfuric acid ethanol solution, hot air Blow until the color of the spots is clear, and in the chromatogram of the test product, the main spots of the same color appear on the position corresponding to the chromatogram of the control medi...

Embodiment 3

[0034] Identification of lizard medicinal ingredients in Jieguxujin Tablets (sugar-coated tablets made according to the Ministry of Health drug standard WS3-B-2226-96).

[0035] Take an appropriate amount of Jiegu Xujin Tablets, remove the coating, pulverize into powder, weigh 1g of the above powder, add 20mL of ethanol, after ultrasonic treatment for 20min, filter, evaporate the filtrate to dryness, add 1mL of methanol to dissolve the residue, and use it as the test solution. Take 0.5 g of the lizard reference medicinal material, and prepare the reference medicinal material solution in the same way.

[0036]Take 1CμL of each of the above two solutions, spot them on the same silica gel G thin-layer plate, use benzene: ethyl acetate = 1:1 as the developer, develop, take out, dry in the air, spray with 10% sulfuric acid ethanol solution, hot air Blow until the color of the spots is clear, and in the chromatogram of the test product, the main spots of the same color appear on the...

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Abstract

The invention relates to a method for identifying a medicinal lizard material, which comprises the following steps of: selecting a reference lizard material as the reference, respectively preparing a test sample solution and a reference material solution, dispensing on the same silica gel G thin layer plate, developing by a developing solvent including toluene or benzene and ethyl acetate with a ratio of 1:(0.8-1.3), adding ethanol sulfate solution so that the test sample and the reference material render the identical color at corresponding positions, and visually inspecting using 365 nm UV light under UV lamp to observe fluorescent spots of the same color of the test sample and the reference material at corresponding positions. The inventive identification method has high specificity and good reproducibility, and is suitable for identification of lizard materials of all resources and for identification of the presence or not of lizard material in products prepared from lizard materials of all resources.

Description

technical field [0001] The invention belongs to the technical field of identification of medicinal materials, and relates to a qualitative analysis method of natural medicinal materials, in particular to an identification method of lizard medicinal materials. Background technique [0002] Lizards have a long history of medicinal use in my country, and there are medicinal records in "Shen Nong's Materia Medica" and "Compendium of Materia Medica". Lizards belong to medicinal materials with multiple names and sources. The name of the medicinal materials recorded on page 217 of "Ministry of Health Drug Standards for Traditional Chinese Medicine Prescriptions" (Volume 11) is "Lizard", which comes from Eremiasargus Peters of the lizard family Lizard lizard. dry body. The name of the medicinal material recorded on page 273 of "Jilin Province Drug Standards" (1977) is "Snama lizard", also known as "Horse snake", which is derived from the dried whole body of Eremias argus Peters, a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N1/38
Inventor 朱平王志敏
Owner 山西华元医药生物技术有限公司
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