The invention discloses a thin-layer
chromatography method for simultaneously identifying four single medicinal materials from Lonicera and
Forsythia powder, which comprises the following steps of: weighing Lonicera and
Forsythia powder, adding
methanol for extracting, filtering, evaporating filtrate to
dryness in a water bath, adding water to dissolve residues, extracting by using a water-saturated n-butyl
alcohol solution, adding water to wash a separated n-butyl
alcohol layer, discarding water liquid, adding
petroleum ether, layering, collecting an upper-layer solution, adding a
methanol solution into the upper-layer solution, washing, collecting an upper-layer solution,
drying by
distillation, and dissolving residues with
methanol to a constant volume of 0.5-2mL. According to the thin-layer
chromatography identification method, as long as one
test solution is prepared, the four medicinal materials including arctii fructus, liquorice,
semen sojae praeparatum and schizonepetae spica can be simultaneously identified from a
complex system of the
traditional Chinese medicine Lonicera and
Forsythia powder under two development conditions. The thin-layer identification time is shortened, the detection efficiency is improved, the consumption of reference substances and reagents is reduced, and the detection cost is greatly reduced.