33results about How to "Negative without interference" patented technology

The invention discloses a quality control method of astragalus-leech capsules capable of regulating collaterals. The quality control method comprises the steps of: respectively differentiating leech, red peony root, astragalus root, ginseng, chuanxiong rhizome, red sage root, ground beetle, cinnamon, borneol and polygonum multiflorum in medicaments by using a thin-layer chromatography; determining the content of astragaloside in the medicaments by using a thin-layer scanning method; and determining the content of tanshinol and paeoniflorin in the medicaments by using a liquid-phase chromatography. According to the quality control method disclosed by the invention, the medicinal material differentiation method is mature, feasible, negative and non-interfering and is strong in specificity, and the content determination method is easy to operate, high in precision and good in reproducibility, so that the quality control method disclosed by the invention can be used for controlling the quality of the medicaments accurately and stably, thereby being adaptable to the industrial stable production of the medicaments.

The invention provides a detection method of a traditional Chinese medicine preparation for preventing infectious bronchitis. The traditional Chinese medicine preparation is prepared from roots of Baikal skullcap, fructus forsythia, cape jasmine fruits, honeysuckle, pogostemon cablin, Chinese thorowax roots and Thunberg fritillary bulb. The quality detection method includes identification and content determination projects. The thin-layer chromatography identification method for seven traditional Chinese medicines is clear in spot, good in repeatability, negative and free from interference, and can serve as a traditional Chinese medicine composition quality control method. A built baicalein content determination method is high in precision and accurate in measuring result, and product quality can be effectively controlled, so that clinical curative effects are ensured.

The invention discloses a quality detection method of a capsule for reducing blood fat and activating collaterals. The capsule for reducing blood fat and activating collaterals is prepared from 100g of cassia seed, 1500g of curcuma, 2000g of rhizoma alismatis, 500g of notoginseng and 2000g of bermudagrass herb. The quality detection method of the capsule for reducing blood fat and activating collaterals additionally provides thin-layer chromatography identification of the rhizoma alismatis on the basis of the primary standard, and the rhizoma alismatis is one of main components of the capsule. After the thin-layer chromatography identification of the rhizoma alismatis is additionally provided on the basis of the primary standard, the purpose of qualitatively controlling the rhizoma alismatis in the preparation is achieved, the quality detection standard of the capsule for reducing blood fat and activating collaterals is perfected, and simultaneously, a scientific basis for judging the truth of products and controlling the quality of products is provided. In the method, a sample test is simple and convenient to operate; and proven by negative interference and other continuous verification tests, the method has the advantages of negative non-interference, good reproducibility and strong specificity. The invention perfects the quality detection standard and improves the control capability of the product quality.

The invention relates to a detection method of a yupingfeng oral liquid. A thin layer chromatography is adopted, the same sample pretreatment method and the same chromatographic condition are used, and astragali radix and saposhnidoviae radix in a yupingfeng oral liquid prescription are simultaneously identified on the same thin layer plate. The method of identifying the astragali radix and the saposhnidoviae radix by the thin layer chromatography comprises the steps of (1) test solution preparation, (2) reference solution preparation, and (3) thin layer chromatography conditions and result. According to the detection method, the quality standard of the yupingfeng oral liquid is improved and perfected, and the identification of the saposhnidoviae radix is revised from a high performance liquid chromatography to a thin layer identification method, so that the identification time is greatly shortened, and the detection cost is lowered. Compared with a method in the prior art, the detection method is simpler, more convenient, rapider and more economical; the quality is easier to control; and the detection method is more suitable for industrial production.

The invention discloses a thin-layer chromatography method for simultaneously identifying four single medicinal materials from Lonicera and Forsythia powder, which comprises the following steps of: weighing Lonicera and Forsythia powder, adding methanol for extracting, filtering, evaporating filtrate to dryness in a water bath, adding water to dissolve residues, extracting by using a water-saturated n-butyl alcohol solution, adding water to wash a separated n-butyl alcohol layer, discarding water liquid, adding petroleum ether, layering, collecting an upper-layer solution, adding a methanol solution into the upper-layer solution, washing, collecting an upper-layer solution, drying by distillation, and dissolving residues with methanol to a constant volume of 0.5-2mL. According to the thin-layer chromatography identification method, as long as one test solution is prepared, the four medicinal materials including arctii fructus, liquorice, semen sojae praeparatum and schizonepetae spica can be simultaneously identified from a complex system of the traditional Chinese medicine Lonicera and Forsythia powder under two development conditions. The thin-layer identification time is shortened, the detection efficiency is improved, the consumption of reference substances and reagents is reduced, and the detection cost is greatly reduced.

The invention relates to a method for identifying a medicinal lizard material, which comprises the following steps of: selecting a reference lizard material as the reference, respectively preparing a test sample solution and a reference material solution, dispensing on the same silica gel G thin layer plate, developing by a developing solvent including toluene or benzene and ethyl acetate with a ratio of 1:(0.8-1.3), adding ethanol sulfate solution so that the test sample and the reference material render the identical color at corresponding positions, and visually inspecting using 365 nm UV light under UV lamp to observe fluorescent spots of the same color of the test sample and the reference material at corresponding positions. The inventive identification method has high specificity and good reproducibility, and is suitable for identification of lizard materials of all resources and for identification of the presence or not of lizard material in products prepared from lizard materials of all resources.

The invention discloses a rapid thin-layer identification method for multiple medicines in common goldenrop granule finished products. The rapid thin-layer identification method comprises the following steps: S1, preparing a reference medicinal material solution, a reference substance solution and a test solution; S2, carrying out thin-layer chromatography identification; and S3, carrying out methodology verification and finished product detection. According to the method, the same test solution and the same developing solvent can be used for simultaneously identifying the bupleurum chinense (monarch drug), scutellaria baicalensis (minister drug) and liquorice (assistant and guide drug) in the common goldenrop granule finished products on a thin-layer plate, so that the identification timeis shortened, the detection efficiency is improved, the consumption of reagents is reduced, and the detection cost is greatly reduced.

The invention discloses an identification method of herba patriniae in a Chinese herbal compound, and particularly relates to a thin-layer identification method of herba patriniae in a Chinese herbal compound. The identification method comprises the following steps of preparing a reference crude drug solution; preparing a test product solution; preparing a negative sample solution; performing sample application on the same silica gel G thin-layer plate with a capillary sample applicator; placing the thin-layer plate into a chromatographic jar, performing expansion with an expanding agent, taking out and drying the thin-layer plate in air; and inspecting a thin-layer chromatographic sheet to obtain a result for identification, wherein a main spot of the same color is developed at a position, which corresponds to a reference crude drug chromatography, on a test product chromatography, but no spot is developed on a negative sample. The identification method disclosed by the invention is simple in technology, good in separation effect and extremely high in specificity; and the accuracy of component identification of herba patriniae in the Chinese herbal compound is further improved, and the identification method has a huge popularization and application potential in the field of identification of herba patriniae-related medicines.

The invention provides a detection method of a medicine for treating epileptoid convulsion, infantile convulsion and mimic convulsion. The detection method comprises a step that Salvia miltiorrhiza in the medicine by adopting thin layer chromatography, wherein thin layer chromatography conditions are characterized in that a silica gel G thin layer plate is adopted, and an A-B-C mixed solution having an A/B/C volume ratio of 70-100:5-10:1-5, preferably 80-100:6-9:1-4, and more preferably 90:8:2 is adopted as a developing solvent; the above A solution is selected from dichloromethane, benzene, toluene, trichloromethane, isopropanol and n-butanol, and preferably trichloromethane; the above B solution is selected from ethanol, ethyl acetate, acetone, glycol, methanol and dioxin, and preferably methanol; and the above C solution is selected from formic acid, acetate acid gracial, dimethyl ether, methyl formate and water, and preferably formic acid. The method allows the quality of the medicine to be effectively controlled, and to be stable, controllable, efficient and safe.