Detection method of Jiuwei Zhuhuang preparation

A technology of Jiuweizhuhuang and its detection method, which is applied in the directions of measuring devices, material analysis through optical means, instruments, etc., and can solve problems such as the absence of Jiuweizhuhuang preparation detection method, etc.

Active Publication Date: 2012-07-18
TIBET QIZHENG TIBETAN MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] At present, there is no detection method for Jiuweizhuhuang preparation, and there is no method for detecting the content of toxic components in it.

Method used

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  • Detection method of Jiuwei Zhuhuang preparation
  • Detection method of Jiuwei Zhuhuang preparation
  • Detection method of Jiuwei Zhuhuang preparation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0091] Experimental example 1: Microscopic identification of safflower and licorice

[0092] (1) Instrument

[0093] Microscopes, cameras, glass slides, coverslips, electronic balances, pipettes, filter paper, sonicators.

[0094] (2) Reagents

[0095] Chloral Hydrate, Glycerin, Distilled Water, Acetic Acid.

[0096] (4) Inspection method:

[0097] Permeabilization solvent: chloral hydrate test solution, glycerin acetate test solution, dilute glycerin and other solutions are permeabilization solvents.

[0098] Permeabilization method: traditional heating permeabilization method and oven heating method.

[0099] The above permeabilization solvents and permeabilization methods were respectively selected for testing, and the results are as follows:

[0100]

[0101] Repeated tests under the above conditions finally determined the specific microscopic identification method of safflower and licorice as follows:

[0102] Take 0.001g of the Jiuweizhuhuang preparation, add ...

experiment example 2

[0103] Experimental example 2: TLC identification of safflower

[0104] (1) Instrument

[0105] Mortar, electronic scale, Erlenmeyer flask with stopper, pipette, ultrasonic extractor, funnel, filter paper, evaporating dish, spotter, silica gel H plate, chromatography cylinder.

[0106] (2) Control medicinal materials

[0107] Safflower (batch number: 120907-200609) was purchased from China National Institute of Pharmaceutical and Biological Products.

[0108] (3) Reagents

[0109] Methanol, ethanol, formic acid, ethyl acetate, water, acetone.

[0110] (4) Inspection method:

[0111] Extraction solvent: ethanol, acetone, 80% acetone, ethyl acetate.

[0112] Extraction method: shaking and cold soaking.

[0113] Developing solvent: ethyl acetate-formic acid-water-methanol (7:2:3:0.4), ethyl acetate-formic acid-water (7:2:3). The above extraction solvents, extraction methods and developing agents were respectively selected for testing, and the results are as follows:

[...

experiment example 3

[0117] Experimental example 3: TLC identification of licorice

[0118] (1) Instrument

[0119] Mortar, electronic scale, measuring cylinder, flat bottom flask, condenser tube, rubber tube, water bath, ultrasonic extractor, funnel, filter paper, evaporating dish, separatory funnel, absorbent cotton, sample spotter, silica gel G plate, chromatography cylinder, ultraviolet spot analyzer.

[0120] (2) Control medicinal materials

[0121] Licorice reference medicinal material (batch number: 120904-200914) was purchased from China National Institute of Pharmaceutical and Biological Products.

[0122] (3) Reagents

[0123] Petroleum ether (60-90°C), benzene, ethyl acetate, glacial acetic acid, methanol, absolute ethanol, chloroform, distilled water, sulfuric acid, anhydrous sodium sulfate.

[0124] (4) Inspection method:

[0125] Extraction solvent: Methanol, chloroform + 2.5mol / L sulfuric acid solution, ethyl acetate + 2.5mol / L sulfuric acid solution, ethyl acetate, chlorofor...

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Abstract

The invention relates to a detection method of a Jiuwei Zhuhuang preparation, comprising one or several kinds of the following identification and / or detection methods: identifying morphological characteristics of liquorice and safflower in the preparation by a microscope; qualitatively identifying the safflower, the liquorice and artificial cow-bezoar in the preparation by thin-layer chromatography; detecting the content of aconitine contained in bonga aconite in the preparation; and measuring the content of the hydroxyl carthamin yellow A contained in the safflower in the preparation by high performance liquid chromatography. The Jiuwei Zhuhuang preparation is effectively detected by the above detection method.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a detection method of traditional Chinese medicine preparations. Background technique [0002] Jiuweizhuhuang powder is a Tibetan medicine, which is recorded in the Tibetan medicine sub-volume of "Drug Standards of the Ministry of Health". The standard number is WS3-BC-0248-95. , Licorice, Congfu, Rabbit Ear Grass, Sandalwood nine herbs. It is mainly used for influenza, pneumonia and upper respiratory tract infection in children. [0003] Tianzhuhuang: It is recorded in the Pharmacopoeia of the People's Republic of China, 2010 edition, page 53. This product is a dried lump of exudate from the rods of Bambusa textilis McClure or Schizostachyum chinense Rendle and other plants of Gramineae. Harvested in autumn and winter. [0004] Safflower: recorded in the Pharmacopoeia of the People's Republic of China, 2010 edition, page 141. This product i...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N30/90G01N30/02
Inventor 刘鸿雁张国霞陈丽娟
Owner TIBET QIZHENG TIBETAN MEDICINE
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