The invention relates to a method for preparing
enzyme-natured
bezoar. In the method, colon bacillus is natured
in vitro; colon bacillus beta-GA
enzyme is extracted and implanted to the
gall of animals, such as cattle, sheep, pig, etc; the effective ingredients of
gall bezoar are catalyzed and totally precipitated, then the
bezoar is prepared. Namely: the colon bacillus is natured in 3.6-4.0% of albumen brothculture at 35-38 DEG C of constant temperature for 24 hours and centrifugally precipitated, then colon bacillus
precipitation is gained; then 10-15g of the colon bacillus
precipitation is added into 400mlpH6.8 of trihydroxymethyl aminomethane-
hydrochloric acid buffer solution, vibrated in an ultrasonic groove for 15-20 minutes and centrifuged, then the colon bacillus beta-GA
enzyme solution is gained; 28-32ml of the solution is added into 3,000ml of the
gall of the animals, such as cattle, sheep, pig, etc, and 20-30g of
borax is added, enzymatic reaction is carried out on the mixture at 35-38 DEG C of constant temperature for 24 hours, the bezoar of the invention is gained. The invention is characterized in that the raw materials are abundant, the technique is simple, the equipment is less, the investment is less, the cost is low, the benefit is high; the
therapeutic effect of the enzyme-natured bezoar is the same as or better than that of the natural bezoar; and the enzyme-natured bezoar can be prepared industrially.