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Quality determination method for external cultivated bezoar

A technology for quality identification and bezoar, applied in the quality field of cultivating bezoar in vitro

Active Publication Date: 2005-03-23
WUHAN JIANMIN DAPENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for studying the fingerprints of bezoar in vitro, which is used for the raw materials of bezoar in vitro and the ingredients containing bezoar The quality identification and identification of in vitro cultured bezoar in the preparation can be used as an effective means to distinguish in vitro cultured bezoar from natural and artificial bezoar

Method used

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  • Quality determination method for external cultivated bezoar
  • Quality determination method for external cultivated bezoar
  • Quality determination method for external cultivated bezoar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) The research method of standard fingerprint of bile acid components of bezoar cultured in vitro

[0049] 1. Preparation of the test solution

[0050] Take 10 batches of in vitro cultured bezoar fine powder or take 0.2 g of preparation powder containing in vitro cultured bezoar, weigh them accurately respectively, add 10.0 ml of a mixed solution of 0-5% formic acid aqueous solution and methanol (v:v=5:95) , placed in a stoppered Erlenmeyer flask for ultrasonic extraction for 10-45 minutes, the extract was placed in a centrifuge tube, centrifuged, the supernatant was filtered through a 0.45 μm filter membrane, the initial filtrate was discarded, and the subsequent filtrate was obtained.

[0051] 2. Preparation of reference solution

[0052]Take an appropriate amount of reference substances of cholic acid and deoxycholic acid, weigh them accurately, dissolve them in methanol, and prepare solutions containing 1 mg of reference substances per ml.

[0053] 3. Detection ...

Embodiment 2

[0098] Embodiment 2, a kind of quality identification method of in vitro cultured bezoar,

[0099] (1) Establishment of fingerprints of bile acids in vitro cultured bezoar

[0100] a, preparation of test solution

[0101] Take more than 10 batches of fine powder of in vitro cultured bezoar or preparation powder containing in vitro cultured bezoar, and accurately weigh them respectively. Add 100 times the amount (ml / g) of a mixed solution of 5% organic acid acetic acid aqueous solution and low-carbon organic alcohol ethanol, put it in a stoppered Erlenmeyer flask for ultrasonic extraction for 10-45 minutes, put the extract in a centrifuge tube, centrifuge, and The clear liquid is filtered with a 0.45 μm filter membrane, the initial filtrate is discarded, and the subsequent filtrate is taken to obtain,

[0102] Wherein the volume ratio of organic acid aqueous solution and low carbon organic alcohol is 5: 95,

[0103] b. Preparation of reference substance solution

[0104] Ta...

Embodiment 3

[0124] A method for identifying the quality of in vitro cultured bezoar,

[0125] (1) Establishment of fingerprints of bile acids in vitro cultured bezoar

[0126] a, preparation of test solution

[0127] Take more than 10 batches of fine powder of in vitro cultured bezoar or preparation powder containing in vitro cultured bezoar, and accurately weigh them respectively. Add 20 times the amount (ml / g) of the mixed solution of 2% organic acid trifluoroacetic acid aqueous solution and low-carbon organic alcohol n-butanol 10.0ml, put it in a stoppered Erlenmeyer flask for ultrasonic extraction for 10-45min, and put the extract in a centrifuge tube , centrifuged, and the supernatant was filtered with a 0.45 μm filter membrane, the initial filtrate was discarded, and the subsequent filtrate was taken to obtain,

[0128] Wherein the volume ratio of organic acid aqueous solution and low-carbon organic alcohol is 10:97,

[0129] b. Preparation of reference substance solution

[013...

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Abstract

The invention discloses a quality identifying method for bezoar which is implanted in vitro, at first, make standard fingerprint of bile acid and bilirubin of bezoar which is implanted in vitro: 1.preparing tested solution and contrapositive solution, 2. reversed phase of chromatographic column to detect its highperformance liquid chromatogram, 3. analyse the data of highperformance liquid chromatogram of more than 10 groups tested solution, in order to establish standard fingerprint of bile acid and bilirubin. Then establish highperformance liquid chromatogram data of tested sample, which is compared and identify with above standard fingerprint. The invention is used to differentiate and identify the quality of bezoar which is implanted in vitro, which is contained in implanted in vitro bezoar material and preparation, which includes implanted in vitro bezoar. The invention can be used as an effective method to differentiate implanted in vitro bezoar and natural bezoar, artificial bezoar. The excellences of the invention are simple, stability, high precision, good reproductivity, easily to be predominated, the style and quality can be know from the whole character appearance of the chromatogram.

Description

technical field [0001] The invention relates to a method for controlling the quality of bezoar bezoar, in particular using fingerprints to identify and control the quality of in vitro cultivated bezoar bezoar raw materials or preparations containing in vitro cultivated bezoar. Background technique [0002] Traditional Chinese medicine fingerprint technology has become a development trend at home and abroad. Chinese medicine fingerprint technology is a new type of standard for the quality of Chinese medicine production, which can ensure the consistency of Chinese medicine products; it is also a characteristic map of component groups, which can fully reflect the types and quantities of chemical components contained in Chinese medicine, and then reflect the quality of Chinese medicinal materials and their products. the quality of. Fingerprints obtain the chemical composition information of different individuals of traditional Chinese medicine, which ar...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/14G01N30/26G01N30/74G01N30/88
Inventor 凌娅丁岗李明慧盛龙生
Owner WUHAN JIANMIN DAPENG PHARMA
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