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Thin-layer chromatography method for simultaneously identifying four single medicinal materials from Lonicera and Forsythia powder

A technique of thin-layer chromatography and Yinqiaosan, applied in the field of thin-layer chromatography, can solve the problems of complicated operation, single index and high cost, and achieve the effects of good repeatability, quality control and lower detection cost.

Active Publication Date: 2021-10-08
SHANDONG ACAD OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of single index, complicated operation and high cost in the method for detecting the quality of Yinqiao powder in the prior art, the present invention provides a thin-layer chromatography method for simultaneously identifying four kinds of single medicinal materials from Yinqiao powder. The method only needs to prepare one test solution, which can satisfy the TLC identification of the four medicinal materials of burdock, licorice, tempeh, and nepeta from the complex system of the traditional Chinese medicine Yinqiaosan under two kinds of development conditions. method

Method used

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  • Thin-layer chromatography method for simultaneously identifying four single medicinal materials from Lonicera and Forsythia powder
  • Thin-layer chromatography method for simultaneously identifying four single medicinal materials from Lonicera and Forsythia powder
  • Thin-layer chromatography method for simultaneously identifying four single medicinal materials from Lonicera and Forsythia powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of the test solution:

[0035]Weigh 10.00g of Yinqiao Powder, add 30ml of methanol, soak for 1h, sonicate for 30min, let it stand at room temperature, filter, add 10mL of methanol to the residue, mix well, filter, combine the filtrate, evaporate the filtrate to dryness in a water bath, add 10mL of water and heat to dissolve, then Add 1mL of purified water to rinse once, and combine the water solution; extract the water solution with water-saturated n-butanol, extract twice, 20mL each time, shake vigorously during extraction, and combine the n-butanol layer; add water to the n-butanol layer and wash twice , 20mL each time, discard the water, and collect the n-butanol layer; add 30mL of petroleum ether (60-90°C) to the n-butanol layer solution, shake until the layers are obvious, and collect the upper layer solution; then add 10% Wash once with 20mL of methanol solution, shake gently to prevent emulsification, collect the upper layer solution, evaporate to...

Embodiment 2

[0044] (1) The preparation method of the test solution is the same as in Example 1;

[0045] (2) The preparation method of the negative control solution is the same as in Example 1;

[0046] (3) The preparation method of the contrast medicinal solution is the same as in Example 1;

[0047] (4) TLC identification:

[0048] Common identification of burdock and licorice: Take 3 μl each of the test solution, the negative control solution lacking burdock, the reference medicinal solution of burdock, the negative control solution lacking licorice, and the reference medicinal solution of licorice, and place them on the same thin-layer plate G respectively. Butyl acetate-95% ethanol-ammonia water (volume ratio is 8:5:0.4) is the developing agent, develop 8cm, take it out, dry it, and put it under a 365nm ultraviolet lamp for observation. At the position of , the same color spot is displayed, but the position of the corresponding spot is R f The value is too small;

[0049] Joint i...

Embodiment 3

[0051] (1) The preparation method of the test solution is the same as in Example 1;

[0052] (2) The preparation method of the negative control solution is the same as in Example 1;

[0053] (3) The preparation method of the contrast medicinal solution is the same as in Example 1;

[0054] (4) TLC identification:

[0055] Common identification of burdock and licorice: Take 3 μl each of the test solution, the negative control solution lacking burdock, the reference medicinal solution of burdock, the negative control solution lacking licorice, and the reference medicinal solution of licorice, and place them on the same thin-layer plate G respectively. Butyl acetate-95% ethanol-ammonia water (volume ratio is 8:2:0.4) is the developing agent, develop 6cm, take it out, dry it, and observe it under a 365nm ultraviolet lamp. At the position of , the corresponding spots cannot be completely separated.

[0056] Joint identification of light tempeh and nepeta ears: absorb 3 μl each o...

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Abstract

The invention discloses a thin-layer chromatography method for simultaneously identifying four single medicinal materials from Lonicera and Forsythia powder, which comprises the following steps of: weighing Lonicera and Forsythia powder, adding methanol for extracting, filtering, evaporating filtrate to dryness in a water bath, adding water to dissolve residues, extracting by using a water-saturated n-butyl alcohol solution, adding water to wash a separated n-butyl alcohol layer, discarding water liquid, adding petroleum ether, layering, collecting an upper-layer solution, adding a methanol solution into the upper-layer solution, washing, collecting an upper-layer solution, drying by distillation, and dissolving residues with methanol to a constant volume of 0.5-2mL. According to the thin-layer chromatography identification method, as long as one test solution is prepared, the four medicinal materials including arctii fructus, liquorice, semen sojae praeparatum and schizonepetae spica can be simultaneously identified from a complex system of the traditional Chinese medicine Lonicera and Forsythia powder under two development conditions. The thin-layer identification time is shortened, the detection efficiency is improved, the consumption of reference substances and reagents is reduced, and the detection cost is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicine components, and in particular relates to a thin-layer chromatography method for simultaneously identifying four kinds of single medicinal materials from Yinqiao powder. Background technique [0002] The quality of traditional Chinese medicine is the premise to ensure the effectiveness and safety of clinical medication, the key factor to ensure the efficacy of traditional Chinese medicine, and the basic guarantee for the modernization and scientificization of traditional Chinese medicine. Therefore, improving the quality control standard of traditional Chinese medicine is particularly important for improving the quality of traditional Chinese medicine. Traditional Chinese medicine compound prescriptions are holistic and systematic in clinical application, but using a single component to evaluate the quality of traditional Chinese medicine lacks comprehensiveness. ...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94G01N30/95G01N30/06G01N30/14G01N30/12
CPCG01N30/90G01N30/94G01N30/95G01N30/06G01N30/14G01N30/12G01N2030/126G01N2030/062
Inventor 张会敏宋健钟方晓董学徐林
Owner SHANDONG ACAD OF CHINESE MEDICINE
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