A detection method for cough medicine
A detection method and drug technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of chemical identification of constituent drugs and quantitative determination of main drugs, etc., to achieve strong specificity, easy operation, and reproducibility Good results
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Embodiment 1
[0023] Example 1: TLC identification of borneol.
[0024] Take 6g of Zhisou Qingguo Pills, add 3g of diatomaceous earth, grind well, add 40ml of chloroform, ultrasonicate for 10 minutes, filter, and evaporate the filtrate to 2ml, as the test solution. Take another borneol reference substance, add chloroform to make a solution containing 0.1mg per 1ml, as the reference substance solution. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2005 edition, Appendix VI B) test, draw 2 μl of each of the above two solutions, respectively spot on the same silica gel G thin-layer plate, add toluene-ethyl acetate (17:1) As a developing agent, develop, take out, dry in the air, spray with 5% phosphomolybdic acid ethanol solution, and heat at 105°C until the spots are clearly colored. In the chromatogram of the test product, the same blue spot appears at the position corresponding to the chromatogram of the reference product.
Embodiment 2
[0025] Embodiment 2: TLC identification of green fruit.
[0026] Take 6g of Zhisou Qingguo Pills, add 3g of diatomaceous earth, grind well, add 30ml of ethyl acetate, heat and reflux in a water bath to extract for 1 hour, filter, recover the solvent from the filtrate to dryness, add 1ml of ethyl acetate to the residue to dissolve, and use it as the test product solution. Take another 0.5g of green fruit reference medicinal material, add 20ml of ethyl acetate, sonicate for 20 minutes, filter, recover the solvent from the filtrate to dryness, add 1ml of ethyl acetate to the residue to dissolve, and use it as the reference medicinal material solution. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2005 edition, appendix VI B) test, absorb 5 μl of the above-mentioned test solution and the control medicinal material solution, respectively spot on the same silica gel G thin-layer plate, with toluene-ethyl acetate -Formic acid (5:4:1) as the developer, develop, ...
Embodiment 3
[0027] Embodiment 3: TLC identification of ephedra.
[0028] Take 5g of Zhisou Qingguo Pills, add 3g of diatomaceous earth, grind well, add 20ml of methanol, ultrasonically treat for 30 minutes, filter, recover the solvent from the filtrate to dryness, add 1ml of methanol to dissolve the residue, and use it as the test solution. Another 1 g of ephedra reference medicinal material was taken, and the reference medicinal material solution was prepared in the same way. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2005 edition, an appendix VI B) test, draw 10 μl of the above-mentioned test solution and 2 μl of the reference medicinal material, respectively spot on the same silica gel G thin-layer plate, and use chloroform-methanol- Concentrated ammonia water (20:5:0.5) was used as the developer, developed, taken out, dried in the air, sprayed with ninhydrin test solution, heated at 105°C until the spots were clearly colored. In the chromatogram of the test p...
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