A detection method for cough medicine

A detection method and drug technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of chemical identification of constituent drugs and quantitative determination of main drugs, etc., to achieve strong specificity, easy operation, and reproducibility Good results

Active Publication Date: 2014-10-29
山西振东五和堂制药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above-mentioned quality standards have relatively serious defects: firstly, the standard does not carry out quantitative determination of the content of active ingredients for the main drugs; secondly, more than 50% of the constituent drugs are not chemically identified

Method used

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  • A detection method for cough medicine
  • A detection method for cough medicine
  • A detection method for cough medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: TLC identification of borneol.

[0024] Take 6g of Zhisou Qingguo Pills, add 3g of diatomaceous earth, grind well, add 40ml of chloroform, ultrasonicate for 10 minutes, filter, and evaporate the filtrate to 2ml, as the test solution. Take another borneol reference substance, add chloroform to make a solution containing 0.1mg per 1ml, as the reference substance solution. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2005 edition, Appendix VI B) test, draw 2 μl of each of the above two solutions, respectively spot on the same silica gel G thin-layer plate, add toluene-ethyl acetate (17:1) As a developing agent, develop, take out, dry in the air, spray with 5% phosphomolybdic acid ethanol solution, and heat at 105°C until the spots are clearly colored. In the chromatogram of the test product, the same blue spot appears at the position corresponding to the chromatogram of the reference product.

Embodiment 2

[0025] Embodiment 2: TLC identification of green fruit.

[0026] Take 6g of Zhisou Qingguo Pills, add 3g of diatomaceous earth, grind well, add 30ml of ethyl acetate, heat and reflux in a water bath to extract for 1 hour, filter, recover the solvent from the filtrate to dryness, add 1ml of ethyl acetate to the residue to dissolve, and use it as the test product solution. Take another 0.5g of green fruit reference medicinal material, add 20ml of ethyl acetate, sonicate for 20 minutes, filter, recover the solvent from the filtrate to dryness, add 1ml of ethyl acetate to the residue to dissolve, and use it as the reference medicinal material solution. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2005 edition, appendix VI B) test, absorb 5 μl of the above-mentioned test solution and the control medicinal material solution, respectively spot on the same silica gel G thin-layer plate, with toluene-ethyl acetate -Formic acid (5:4:1) as the developer, develop, ...

Embodiment 3

[0027] Embodiment 3: TLC identification of ephedra.

[0028] Take 5g of Zhisou Qingguo Pills, add 3g of diatomaceous earth, grind well, add 20ml of methanol, ultrasonically treat for 30 minutes, filter, recover the solvent from the filtrate to dryness, add 1ml of methanol to dissolve the residue, and use it as the test solution. Another 1 g of ephedra reference medicinal material was taken, and the reference medicinal material solution was prepared in the same way. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2005 edition, an appendix VI B) test, draw 10 μl of the above-mentioned test solution and 2 μl of the reference medicinal material, respectively spot on the same silica gel G thin-layer plate, and use chloroform-methanol- Concentrated ammonia water (20:5:0.5) was used as the developer, developed, taken out, dried in the air, sprayed with ninhydrin test solution, heated at 105°C until the spots were clearly colored. In the chromatogram of the test p...

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Abstract

The invention discloses a quality control method of a drug for treating cough. The quality control method comprises the following steps of: respectively indentifying borneol, immature fruits of terminalia chebula, ephedra, root barks of white mulberry and radix scutellariae in the drug by using thin layer chromatography, and measuring the content of ephedrine hydrochloride in the drug by using liquid chromatography. The quality control method established by the invention has the advantages that a crude drug indentifying method is mature and feasible, strong in specificity, negative and free of interference; a content measuring method is easy to operate, high in precision and good in repeatability; and by using the quality control method disclosed by the invention, the quality of the drug can be precisely and stably controlled so that the requirement for stable industrial production of the drug is met.

Description

technical field [0001] The invention relates to a traditional Chinese medicine for treating cough, in particular to a quality control method of the medicine. Background technique [0002] Zhisou Qingguo Pill is a cough medicine, which is used for cough with excessive phlegm, fullness in the chest and diaphragm, shortness of breath and wheezing, dry mouth and throat caused by wind-cold constricting the lungs. [0003] The current implementation standards of Zhisou Qingguo Pills are included in the tenth volume of the Ministry of Health's Drug Standards for Traditional Chinese Medicine Prescriptions. The following are specified in this quality standard. [0004] 1) Take this product and observe under a microscope: the parenchyma cells around the cellulose contain square crystals of calcium oxalate, forming crystal fibers. Calcium oxalate cluster crystals are distributed in mesophyll cells with a diameter of 2-10um. Starch granules are oval, 35-48um in diameter, umbilicus-sh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/02
Inventor 王六贵吕建英
Owner 山西振东五和堂制药有限公司
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