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Agricultural phytocide, preparation method and application thereof

A herbicide and adjuvant technology, applied in the field of pesticides, can solve the problems of application reports in the field of undiscovered pesticides, and achieve the effects of safe use, wide herbicidal spectrum, and low environmental pollution

Inactive Publication Date: 2008-08-20
SHANGHAI PESTICIDE RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the compound is limited to application reports in the field of medicine, and no application reports in the field of pesticides have been found.

Method used

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  • Agricultural phytocide, preparation method and application thereof
  • Agricultural phytocide, preparation method and application thereof
  • Agricultural phytocide, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Microbial fermentation to obtain SPRI-70014

[0033] 1.1. Microbial fermentation

[0034] Glucose 3%, starch 5%, peanut cake powder 1%, soybean cake powder 2%, peptone 0.1%, yeast extract 0.4%, sodium chloride 0.2%, magnesium sulfate 0.5%, ammonium nitrate 0.2%, dipotassium hydrogen phosphate The proportion of 0.05% is made into a culture medium, the pH is adjusted to 7.2, and it is sterilized. Streptomyces gray (S. griseolus) CGMCC No.1370 strain growing on the Gaussian slant was inoculated in the medium, and cultured at 28°C for 36-40 hours;

[0035] The culture is used as a seed, inoculated at a ratio of 5-20% in the fermentation broth composed of the above-mentioned culture medium, and cultured with aeration and stirring at 28° C. for 90-96 hours.

[0036] 1.2. Extraction

[0037] Take 10 kg of the fermentation broth, filter to obtain 9 kg of supernatant, and then extract twice with 18 kg of n-butanol. The organic phase was concentrated to obtain 50 g...

Embodiment 2

[0053] Embodiment 2: the preparation of agricultural herbicide

[0054] 2.1. Preparation of wettable powder

[0055] Get 20 parts of I, II, III or IV obtained in Example 1 (weight ratio, the same below) and mix with 1.5 parts of sodium lignosulfonate, 3 parts of pulling powder, 1.5 parts of sizing agent LS and 74 parts of diatomaceous earth, Pulverize to get 100 parts of 20% wettable powder.

[0056] Get 30 parts of I, II, III or IV obtained in Example 1 (weight ratio, the same below) and mix with 2 parts of sodium lignosulfonate, 4 parts of pulling powder, 2 parts of sizing agent LS and 62 parts of diatomaceous earth, Pulverize to get 100 parts of 30% wettable powder.

[0057] 2.2. Preparation of EC

[0058] Mix 0.5 parts of I, II, III or IV obtained in Example 1 with 3 parts of ethylene glycol, 10 parts of dimethylformamide, 35 parts of xylene and 51.5 parts of ethanol to obtain 100 parts of 0.5% emulsifiable concentrate .

[0059] Mix 5 parts of I, II, III or IV obtain...

Embodiment 3

[0066] Embodiment 3: 0.5% 70014 emulsifiable concentrate formulation herbicidal spectrum test

[0067] According to Example 3, 0.5% emulsifiable concentrate preparation was prepared for experimentation. The potted greenhouse method was used to treat each weed target with different doses of soil and plant spraying. Each treatment was repeated twice, and a blank control was set up. The spraying device is an AJXP-1098 automatic spraying tower, the spraying area is 0.097m2, the amount of liquid medicine is 10ml, the working pressure is 0.2MPa, and the penetration rate is 40%. After the treatment, it was grown in a greenhouse, and the symptoms of plant reactions were observed regularly, and the comprehensive herbicidal activity was visually evaluated according to the 0-100% grading method about 3 and 10 days after the application. The evaluation criteria were shown in Table 1. After 10 days, the herbicidal activity was continued to be observed.

[0068] Table 1. Evaluation criteria...

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Abstract

The invention provides a new herbicide taking the metabolite SPIR-70014 of the Streptomyces griseolus as effective ingredient; wherein the preparation method thereof comprises following steps: separating and extracting herbicidal active ingredient from microbiological culture of S.grieuslus CGMCC No.1370; adding proper carrier and additives to obtain the final herbicide product. The new herbicide has the advantages of low dosage, broad weed control spectrum, safety to environment and wide application to nonselective weeding in paddy field and dry field.

Description

technical field [0001] The invention belongs to the field of pesticides, and in particular relates to an agricultural herbicide using the metabolite SPRI-70014 of Streptomyces grizzius as an active ingredient, a preparation method thereof, and an application of the herbicide. Background technique [0002] Since chemical herbicides began to be used in the 1940s, chemical herbicides have become an important means of weeding farmland to reduce labor intensity and improve work efficiency. At present, chemical herbicides occupy most of the market, but with the continuous application of chemical herbicides , has clearly exposed its disadvantages. First, the herbicide droplet drift, the volatilization of the liquid, the selectivity is not ideal, and the harm of the degradation product causes the phytotoxicity of the crops; secondly, the repeated use of the herbicide causes the population of drug-resistant weeds to increase. , The emergence of herbicide-resistant weeds and the incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P13/00A01N25/30A01N25/08
Inventor 顾学斌陶黎明张育雷叶高艺郭磊徐文平王磊沈晓霞郑佳玲
Owner SHANGHAI PESTICIDE RES INST
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