Cultivation method for north America rhododendron
A technology for tissue culture and azalea, which is applied to the tissue culture field of Rhododendron in North America, can solve the problems of introduction and domestication, breeding of new varieties, lag in reproduction and promotion, and inability to provide scientific and technological support, and achieve the effect of improving the reproduction coefficient.
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Embodiment 1
[0016] A method for tissue culture of North American rhododendrons. The raw material is rhododendron explants. The first step is to collect the winter buds of North American rhododendrons, wash them with clean water, put them on an ultra-clean workbench, and sterilize them with 75% alcohol 3 to 4 seconds, rinsed with sterile water for 3 times, then sterilized with 0.1% mercuric chloride for 6 to 8 minutes, rinsed with sterile water for 3 times; the second step is to remove the scales of the treated winter buds and implant them for bud induction In the culture medium, the bud induction medium is: Read+2.0mg ZT+0.3mg IAA, then add agar 6.5g / L, sucrose 20g / L, pH value is 5.4, after bottling, it is sterilized by high pressure for 15-20 minutes, Put it under the conditions of room temperature 25°C-28°C, light intensity 2000-5000lux, and light time 12 hours a day for bud induction culture. After 30 days, transfer it to the proliferation medium. The proliferation medium is: Read+3.8mg...
Embodiment 2
[0018] A kind of tissue culture method of North American Rhododendron, raw material is Rhododendron explant material, the first step, gathers the spring tender stem section of North American Rhododendron, washes with clear water, puts on the ultra-clean workbench, first uses 75 % alcohol for 3 to 4 seconds, rinsed with sterile water for 3 times, then sterilized with 0.1% mercury liter for 6 to 8 minutes, and rinsed with sterile water for 3 times; in the second step, cut the treated young stems About 1cm long, implanted in the bud induction medium, the bud induction medium is: Read+2.5mg ZT+0.35mg IAA, then add agar 6.5g / L, sucrose 20g / L, pH value is 5.4, after bottling After autoclaving for 15 to 20 minutes, place it at room temperature of 25°C to 28°C, light intensity of 2000 to 5000lux, and light time of 12 hours per day for bud induction culture, and transfer it to the proliferation medium after 30 days to proliferate The medium is: Read+4.5mg ZT+0.60mg IAA, add agar 6.5g / L...
Embodiment 3
[0020] A kind of tissue culture method of North American rhododendron, the raw material is rhododendron explant material, the first step, gathers the spring young leaves of North American rhododendron, washes with clear water, puts on the ultra-clean workbench, first uses 75% Alcohol disinfection for 3 to 4 seconds, rinsed with sterile water for 3 times, then sterilized with 0.1% mercury liter for 6 to 8 minutes, rinsed with sterile water for 3 times; the second step is to cut the treated spring young leaves into about 1cm 2 Size cubes, implanted in the bud induction medium, the bud induction medium is: Read + 3.0mg ZT + 0.45mg IAA, then add agar 6.5g / L, sucrose 20g / L, pH value is 5.4, after bottling, it is subjected to high pressure Sterilize for 15 to 20 minutes, place it at room temperature of 25°C to 28°C, light intensity of 2000 to 5000lux, and light time of 12 hours per day to conduct bud induction culture, and transfer it to the proliferation medium after 30 days. It i...
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