Cultivation method for north America rhododendron

A technology for tissue culture and azalea, which is applied to the tissue culture field of Rhododendron in North America, can solve the problems of introduction and domestication, breeding of new varieties, lag in reproduction and promotion, and inability to provide scientific and technological support, and achieve the effect of improving the reproduction coefficient.

Inactive Publication Date: 2008-10-29
CHINA PAULOWNIA RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And this kind of evergreen rhododendron in my country is mainly distributed in southwestern alpine regions such as Yunnan, Sichuan, and Guizhou. At present, there is a lack of cold-resistant evergreen rhododendron species in the central and northern re...

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0016] A method for tissue culture of North American rhododendrons. The raw material is rhododendron explants. The first step is to collect the winter buds of North American rhododendrons, wash them with clean water, put them on an ultra-clean workbench, and sterilize them with 75% alcohol 3 to 4 seconds, rinsed with sterile water for 3 times, then sterilized with 0.1% mercuric chloride for 6 to 8 minutes, rinsed with sterile water for 3 times; the second step is to remove the scales of the treated winter buds and implant them for bud induction In the culture medium, the bud induction medium is: Read+2.0mg ZT+0.3mg IAA, then add agar 6.5g / L, sucrose 20g / L, pH value is 5.4, after bottling, it is sterilized by high pressure for 15-20 minutes, Put it under the conditions of room temperature 25°C-28°C, light intensity 2000-5000lux, and light time 12 hours a day for bud induction culture. After 30 days, transfer it to the proliferation medium. The proliferation medium is: Read+3.8mg...

Embodiment 2

[0018] A kind of tissue culture method of North American Rhododendron, raw material is Rhododendron explant material, the first step, gathers the spring tender stem section of North American Rhododendron, washes with clear water, puts on the ultra-clean workbench, first uses 75 % alcohol for 3 to 4 seconds, rinsed with sterile water for 3 times, then sterilized with 0.1% mercury liter for 6 to 8 minutes, and rinsed with sterile water for 3 times; in the second step, cut the treated young stems About 1cm long, implanted in the bud induction medium, the bud induction medium is: Read+2.5mg ZT+0.35mg IAA, then add agar 6.5g / L, sucrose 20g / L, pH value is 5.4, after bottling After autoclaving for 15 to 20 minutes, place it at room temperature of 25°C to 28°C, light intensity of 2000 to 5000lux, and light time of 12 hours per day for bud induction culture, and transfer it to the proliferation medium after 30 days to proliferate The medium is: Read+4.5mg ZT+0.60mg IAA, add agar 6.5g / L...

Embodiment 3

[0020] A kind of tissue culture method of North American rhododendron, the raw material is rhododendron explant material, the first step, gathers the spring young leaves of North American rhododendron, washes with clear water, puts on the ultra-clean workbench, first uses 75% Alcohol disinfection for 3 to 4 seconds, rinsed with sterile water for 3 times, then sterilized with 0.1% mercury liter for 6 to 8 minutes, rinsed with sterile water for 3 times; the second step is to cut the treated spring young leaves into about 1cm 2 Size cubes, implanted in the bud induction medium, the bud induction medium is: Read + 3.0mg ZT + 0.45mg IAA, then add agar 6.5g / L, sucrose 20g / L, pH value is 5.4, after bottling, it is subjected to high pressure Sterilize for 15 to 20 minutes, place it at room temperature of 25°C to 28°C, light intensity of 2000 to 5000lux, and light time of 12 hours per day to conduct bud induction culture, and transfer it to the proliferation medium after 30 days. It i...

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Abstract

Disclosed is a method for tissue culture of rhododendron. The tissue culture method comprises the steps that: step 1: rhododendron explant material is collected, and placed on a supper-clean bench after being treated; the rhododendron explant material is disinfected by alcohol with a concentration of 75 percent for 6 to 8 seconds, washed by sterile water for three times, disinfected by 0.001 litre of mercury for 6 to 8 minutes, and washed by sterile water for three times in order; step 2: the material after being disinfected and cut is implanted into bud induce culture medium for thirty days; 30 days later, the obtained material is then implanted into proliferation culture medium for 60 days; step 3: healthy and strong rhododendron seedlings cut with 2 to 3cm by height are implanted into a rooting culture medium for 45 days; step 4: after the newborn root of rhododendron is about 0.5 cm long, the culture medium attached to the root is cleaned and newborn root is moved into sterile thick sand, the air humidity of which is kept ranging from 75 percent to 85 percent; one week later, the newborn root is moved into the mixed material of turfy soil and perlite; 45 days later, the treated root is moved into the field where the root is under the shade for one week. The method can realize industrial culture of seedling within 6 to 7 mouths and mass production of shipshape nursery stock of rhododendron.

Description

Technical field: [0001] The invention relates to a rapid propagation technique of North American rhododendrons, in particular to a tissue culture method of North American rhododendrons, which is suitable for rapid propagation of rhododendrons. Background technique: [0002] Rhododendron is a world-famous woody ornamental flower, belonging to Ericaceae and Rhododendron. Among them, the evergreen rhododendrons account for about one-third of the world's rhododendron natural resources, mainly distributed in the temperate zone of the northern hemisphere, namely Asia, Europe, North America and other places. This North American Rhododendron cultivated by the present invention belongs to the cold-resistant type evergreen large-leaved Rhododendron. It is favored by people because of its large and shiny leaves, many flowers in one bud, bright and colorful, and strong ornamental value. It is popular in the world's ornamental plant market. occupies an important position. And this kind...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00C12N5/04
Inventor 傅建敏杜兰英李芳东杨绍彬李福海金世海夏士文李树战孙志强
Owner CHINA PAULOWNIA RES CENT
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