Hepatitis B e antigen immune escaping detecting gene chip and kit thereof
A technology for detecting gene chips and antigen immunity, which is applied in the field of hepatitis B e antigen immune evasion detection gene chips and kits thereof, can solve the problems of missed detection, unfavorable infectivity of patients and conditions after treatment, and achieves the problem of avoiding missed detection. Effect
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Embodiment 1
[0038] Test each blood sample according to the following method:
[0039] 1. Process serum samples according to the product instructions to extract viral nucleic acid.
[0040] 2. Add PCR amplification solution, amplify and label the processed nucleic acid sample, and use BIOTIN-labeled primers to label the product with biotin.
[0041] 3. Add 10-20ul to the reaction area.
[0042] 4. 42°C heat preservation and moisturizing reaction for 60 minutes.
[0043] 5. Wash 4-6 times with lotion, 2 minutes each time, shake.
[0044] 6. Add affinity reaction solution, 10-20ul.
[0045] 7. React at 42°C for 15-20min.
[0046] 8. Wash 4-6 times with lotion, 2 minutes each time, shake.
[0047] 9. Add detection solution 20ul / well, so that the detection solution is evenly distributed in the reaction area.
[0048] 10. CCD detection, exposure for 30-60 seconds depending on the strength of the luminescent signal.
Embodiment 2
[0050] Test each blood sample according to the following method:
[0051] 1. Process serum samples according to the product instructions to extract viral nucleic acid.
[0052] 2. Add PCR amplification solution, amplify and label the processed nucleic acid samples, and use fluorescently labeled primers to
[0053] The product is labeled with fluorescence.
[0054] 3. Add 10-20ul to the reaction area.
[0055] 4. 42°C heat preservation and moisturizing reaction for 60 minutes.
[0056] 5. Wash 4-6 times with lotion, 2 minutes each time, shake.
[0057] 6. Use a confocal fluorescence microscope or a fluorescence scanner to detect and obtain the results.
[0058] Result judgment:
[0059] According to the CutOff value of different indicators, refer to the gradient curve of the standard to determine the negative or positive of the sample serum.
[0060] At the same time, the serum samples tested were routinely tested by conventional methods. The result shows that the test r...
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