Hepatitis B e antigen immune escaping detecting gene chip and kit thereof

A technology for detecting gene chips and antigen immunity, which is applied in the field of hepatitis B e antigen immune evasion detection gene chips and kits thereof, can solve the problems of missed detection, unfavorable infectivity of patients and conditions after treatment, and achieves the problem of avoiding missed detection. Effect

Active Publication Date: 2008-12-31
上海裕隆生物科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Hepatitis B e antigen immune evasion can cause missed detection of hepatitis B e antigen in clinical serology, which is not conducive to judging the infectivity o

Method used

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  • Hepatitis B e antigen immune escaping detecting gene chip and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Test each blood sample according to the following method:

[0039] 1. Process serum samples according to the product instructions to extract viral nucleic acid.

[0040] 2. Add PCR amplification solution, amplify and label the processed nucleic acid sample, and use BIOTIN-labeled primers to label the product with biotin.

[0041] 3. Add 10-20ul to the reaction area.

[0042] 4. 42°C heat preservation and moisturizing reaction for 60 minutes.

[0043] 5. Wash 4-6 times with lotion, 2 minutes each time, shake.

[0044] 6. Add affinity reaction solution, 10-20ul.

[0045] 7. React at 42°C for 15-20min.

[0046] 8. Wash 4-6 times with lotion, 2 minutes each time, shake.

[0047] 9. Add detection solution 20ul / well, so that the detection solution is evenly distributed in the reaction area.

[0048] 10. CCD detection, exposure for 30-60 seconds depending on the strength of the luminescent signal.

Embodiment 2

[0050] Test each blood sample according to the following method:

[0051] 1. Process serum samples according to the product instructions to extract viral nucleic acid.

[0052] 2. Add PCR amplification solution, amplify and label the processed nucleic acid samples, and use fluorescently labeled primers to

[0053] The product is labeled with fluorescence.

[0054] 3. Add 10-20ul to the reaction area.

[0055] 4. 42°C heat preservation and moisturizing reaction for 60 minutes.

[0056] 5. Wash 4-6 times with lotion, 2 minutes each time, shake.

[0057] 6. Use a confocal fluorescence microscope or a fluorescence scanner to detect and obtain the results.

[0058] Result judgment:

[0059] According to the CutOff value of different indicators, refer to the gradient curve of the standard to determine the negative or positive of the sample serum.

[0060] At the same time, the serum samples tested were routinely tested by conventional methods. The result shows that the test r...

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PUM

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Abstract

The invention provides a hepatitis B virus e antigen immune evasion detection gene chip and a kit, belonging to the gene chip technique field of clinical detection, and comprising: a gene chip of a specific DNA probe which detects hepatitis B virus e antigen immune evasion and is fixed on a solid phase carrier as well as a matched reagent; the matched reagent includes a treating fluid for treating serum samples, an amplifying solution for amplifying and labeling the treated samples, a hybridization solution in hybridization reaction with a micro-array, a cleaning solution, an enzyme-labeled working solution and a detection liquid; and the matched reagent and the hepatitis B virus e antigen immune evasion detection gene chip are used jointly in the detection process of the serum samples. The chip and the kit can detect the hepatitis B virus e antigen immune evasion, and avoid the clinical hepatitis B virus e antigen leak detection, thereby being favorable for judging the infectivity and conditions after curing of hepatitis B patients.

Description

technical field [0001] The invention belongs to the technical field of gene chips for clinical detection, and in particular relates to a gene chip for detecting hepatitis B e antigen immune evasion and a kit thereof. Background technique [0002] my country is the country with the most severe HBV infection in the world. Usually, two-and-a-half detection of hepatitis B is an important part of clinical diagnosis and blood screening. Hepatitis B e antigen is an important indicator in the detection. The e antigen is derived from the core of the hepatitis B virus. The detection of e-antigen is equivalent to the detection of the core antigen, which means that the virus is actively replicating and highly contagious. To know whether HBsAg-positive people are contagious, the more commonly used and reliable detection method is to test e-antigen and e-antibody. Hepatitis B e antigen immune evasion can lead to missed detection of hepatitis B e antigen in clinical serology, which is ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/70
Inventor 穆海东汪宁梅黎飒罗芳英
Owner 上海裕隆生物科技有限公司
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