Eperythrozoonosis rapid diagnosis test paper

Abstract

The invention relates to a rapid diagnosis test strip for the eperythrozoonosis and comprises a support layer at the bottom, a middle adsorption layer and an external protective layer; wherein, the adsorption layer is fixed on the support layer and a fibrous layer, an adsorption colloidal-gold double anti fibrous layer or a colloidal-gold antigen fibrous layer and a cellulose film and a layer of water absorbent material are sequentially arranged from a test end on the adsorption layer; the cellulose film is provided with detection blots printed by specific purification antigen solutions of the eperythrozoon of human beings or an animal, comparison blots printed by solutions of goat anti-rabbit IgG or rabbit anti-goat IgG or comparison blots printed by IgG solutions of eperythrozoon antigens of rabbit (goat) anti human beings or other animals; the detection blots and the comparison blots are sequentially arranged. The diagnosis strip is higher in specificity and sensitivity detection and can detect 2 nanograms of correspondent antibody protein; the strip is simple and rapid in operation, visual and accurate in result display and lower in diagnosis cost and is particularly suitable for the rapid diagnosis of the eperythrozoonosis of human beings or animals on site.

Description

1. Technical field:

[0001] The invention relates to a diagnostic tool for zoonotic infectious diseases, in particular to a rapid diagnostic test strip for Eperythrozoonosis. 2. Background technology:

[0002] Eperythrozoonosis is a zoonotic disease caused by Eperythrozoon parasitizing on the surface of red blood cells, plasma and bone marrow of humans and animals. It is generally a recessive infection. In acute onset, it mainly manifests as anemia and jaundice. , fever, and swollen lymph nodes. Eperythrozoon hosts include rodents, pigs, cattle, sheep, goats, dogs, cats, rabbits, birds, horses, donkeys, mules, camels and humans. Among livestock and poultry, pig and sheep Eperythrozoon are more pathogenic. At present, Eperythrozoonosis exists widely in all parts of the world. Since 1981, the disease has spread and prevailed in many provinces of our country, causing huge economic losses. So far, no vaccine for this disease has been produced or used clinically.

[0003] A p...

Examples

preparation example Construction

[0032] The preparation of the specific purified antigen of porcine eperythrozoon can also use the water bath method. Take positive porcine anticoagulant blood, wash with pH 7.2 PBS 3 times as above, add a little pH 7.2 PBS solution to the red blood cell sludge, put it in a water bath for 3-5 minutes, and centrifuge at 4000r / min for 30 minutes to get the supernatant, supernatant Centrifuge at 20000r / min for 20min, and collect the precipitate as Eperythrozoon. The Eperythrozoa was washed 3 times with pH6.4 PBS solution, centrifuged at 20000r / min for 20min, the precipitate was collected, added a little pH6.4PBS solution, freeze-thawed and pulverized as above, and finally the specific purified antigen of porcine Eperythrozoa was obtained.

[0033] 3.2 Preparation of Eperythrozoon Antigen by Infecting Experimental Animals

[0034] In Kunming mice or rabbits, their immunity was reduced by removing the spleen or injecting dexamethasone, and then infected with Eperythrozoon by intrap...

Embodiment 1

[0044] Embodiment one: see figure 1 , figure 2 . In the figure 1 is the support layer, made of plastic sheet strips, 2 is the fiber layer at the test end of the sample, made of glass wool, and 3 is the fiber layer adsorbed with gold-labeled antibody. In this example, rabbit antibody adsorbed with colloidal gold The glass wool of people's IgG antibody liquid, its preparation method sees the method described in the above-mentioned embodiment 4 to prepare gold-labeled antibody glass wool, 4 is a cellulose membrane layer, this example adopts a nitrocellulose membrane, and 5 is a water-absorbing material layer , made of water-absorbing filter paper, the layers numbered 2, 3, 4, and 5 are pasted on the plastic sheet strip 1 from left to right, and the fibers at the junction between each other cross and penetrate each other. On the nitrocellulose membrane layer 4, 6 is the detection blot "|" printed with human Eperythrozoon-specific purified antigen solution, and 7 is the control ...

Embodiment 2

[0046] Embodiment two: the rapid diagnosis test strip structure is basically the same as embodiment one, the difference is: the fiber layer 3 of the gold-labeled antibody, this example uses the glass wool of the rabbit anti-sheep IgG antibody liquid adsorbed with colloidal gold , on the nitrocellulose membrane layer 4, 6 is the detection blot "|" printed with the specific purified antigen solution of Eperythrozoon ovis, 7 is the control blot "|" printed with the chicken anti-rabbit IgG solution, and others include the detection sample The preparation, operation method and result judgment are the same as the operation method in Embodiment 6, and will not be repeated.

1. Technical field:

[0001] The invention relates to a diagnostic tool for zoonotic infectious diseases, in particular to a rapid diagnostic test strip for Eperythrozoonosis. 2. Background technology:

[0002] Eperythrozoonosis is a zoonotic disease caused by Eperythrozoon parasitizing on the surface of red blood cells, plasma and bone marrow of humans and animals. It is generally a recessive infection. In acute onset, it mainly manifests as anemia and jaundice. , fever, and swollen lymph nodes. Eperythrozoon hosts include rodents, pigs, cattle, sheep, goats, dogs, cats, rabbits, birds, horses, donkeys, mules, camels and humans. Among livestock and poultry, pig and sheep Eperythrozoon are more pathogenic. At present, Eperythrozoonosis exists widely in all parts of the world. Since 1981, the disease has spread and prevailed in many provinces of our country, causing huge economic losses. So far, no vaccine for this disease has been produced or used clinically.

[0003] A p...

Examples

preparation example Construction

[0032] The preparation of the specific purified antigen of porcine eperythrozoon can also use the water bath method. Take positive porcine anticoagulant blood, wash with pH 7.2 PBS 3 times as above, add a little pH 7.2 PBS solution to the red blood cell sludge, put it in a water bath for 3-5 minutes, and centrifuge at 4000r / min for 30 minutes to get the supernatant, supernatant Centrifuge at 20000r / min for 20min, and collect the precipitate as Eperythrozoon. The Eperythrozoa was washed 3 times with pH6.4 PBS solution, centrifuged at 20000r / min for 20min, the precipitate was collected, added a little pH6.4PBS solution, freeze-thawed and pulverized as above, and finally the specific purified antigen of porcine Eperythrozoa was obtained.

[0033] 3.2 Preparation of Eperythrozoon Antigen by Infecting Experimental Animals

[0034] In Kunming mice or rabbits, their immunity was reduced by removing the spleen or injecting dexamethasone, and then infected with Eperythrozoon by intrap...

Embodiment 1

[0044] Embodiment one: see figure 1 , figure 2 . In the figure 1 is the support layer, made of plastic sheet strips, 2 is the fiber layer at the test end of the sample, made of glass wool, and 3 is the fiber layer adsorbed with gold-labeled antibody. In this example, rabbit antibody adsorbed with colloidal gold The glass wool of people's IgG antibody liquid, its preparation method sees the method described in the above-mentioned embodiment 4 to prepare gold-labeled antibody glass wool, 4 is a cellulose membrane layer, this example adopts a nitrocellulose membrane, and 5 is a water-absorbing material layer , made of water-absorbing filter paper, the layers numbered 2, 3, 4, and 5 are pasted on the plastic sheet strip 1 from left to right, and the fibers at the junction between each other cross and penetrate each other. On the nitrocellulose membrane layer 4, 6 is the detection blot "|" printed with human Eperythrozoon-specific purified antigen solution, and 7 is the control ...

Embodiment 2

[0046] Embodiment two: the rapid diagnosis test strip structure is basically the same as embodiment one, the difference is: the fiber layer 3 of the gold-labeled antibody, this example uses the glass wool of the rabbit anti-sheep IgG antibody liquid adsorbed with colloidal gold , on the nitrocellulose membrane layer 4, 6 is the detection blot "|" printed with the specific purified antigen solution of Eperythrozoon ovis, 7 is the control blot "|" printed with the chicken anti-rabbit IgG solution, and others include the detection sample The preparation, operation method and result judgment are the same as the operation method in Embodiment 6, and will not be repeated.

Images