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Preparation and application of a polysaccharide extract from Dendrobium nobile

A technique for extracting polysaccharides and Dendrobium nobile, which is applied in the field of preparation of polysaccharide extracts from Dendrobium nobile, can solve the problems of rough separation and purification process, unclear material basis, uncontrollable quality, etc. Quality Controlled Effects

Inactive Publication Date: 2012-02-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Dendrobium polysaccharides has disadvantages such as unclear material basis, uncontrollable quality, rough separation and purification process, etc., lack of literature and patent reports with high active ingredient content, clear chemical composition, and significant pharmacodynamic activity.
And, also do not see the appearance of the medicine or the pharmaceutical composition about Dendrobium nobile polysaccharide on the market

Method used

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  • Preparation and application of a polysaccharide extract from Dendrobium nobile
  • Preparation and application of a polysaccharide extract from Dendrobium nobile
  • Preparation and application of a polysaccharide extract from Dendrobium nobile

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Preparation of Dendrobium nobile polysaccharide extract

[0020] (1) Degreasing: Take the decoction pieces of Dendrobium nobile, soak them with 8 times the amount of ethanol, reflux twice, filter, and evaporate the organic solvent to get the medicinal residues; (2) Extraction: soak the above medicinal residues with 10 times the amount of water, and put them in a water bath at 50°C Extract, filter, add 10 times the amount of water to the dregs, extract 3 times at 100°C for 1 hour each time, filter, concentrate the filtrate to 1.0 g of crude drug per ml; (3) Alcohol precipitation: add appropriate amount of Ethanol until the ethanol content is 80%, put it in an ice bath for 12 hours, filter, precipitate and freeze-dry the polysaccharide extract crude product I; (4) remove protein: take the above-mentioned polysaccharide extract crude product I and dissolve it in an appropriate amount in water, add appropriate amount of chloroform and normal Butanol, layered after...

Embodiment 2

[0021] The preparation of embodiment two Dendrobium nobile polysaccharide extract

[0022] (1) Degreasing: take the decoction pieces of Dendrobium nobile, soak them with 10 times the amount of 80% ethanol and reflux twice, filter, and evaporate the organic solvent to get the dregs; Extract twice in a water bath at ℃, 1 hour each time, filter, add 8 times the amount of water to the medicinal residue, extract in a water bath at 100 °C twice, 1 hour each time, filter, and concentrate the filtrate to 1.5g crude drug per ml; (3) Alcohol precipitation: add an appropriate amount of ethanol to the above concentrated solution until the ethanol content is 80%, place it in an ice bath for 24 hours, filter, precipitate and freeze-dry the crude polysaccharide extract I; (4) remove protein: take the above polysaccharide extract crude product I Dissolve an appropriate amount in water, add an appropriate amount of chloroform and n-butanol, stir and layer, repeat the operation for the upper la...

Embodiment 3

[0023] The preparation of embodiment three Dendrobium nobile polysaccharide extract

[0024] (1) Degreasing: Weigh 4 kg of Dendrobium medicinal material (dried product, block decoction pieces), add 8 times the amount of 80% ethanol to soak for 3 hours (W / V), reflux at 85° C. for 5 hours, twice. Filter, spread the dregs evenly, and evaporate the ethanol; (2) Extraction: evaporate the dregs until there is no alcohol smell, add 4 times the amount of distilled water (W / V), soak for 12 hours, extract in a water bath at 50°C for 2 hours, twice. Four layers of gauze Buchner funnel suction filtration, add 4 times the amount of distilled water (W / V) to the dregs, and extract with boiling water at 100°C for 2 hours, twice. Filter through a four-layer gauze Buchner funnel, collect the filtrate, combine, and concentrate to 3000ml by rotary evaporation at 60°C; (3) alcohol precipitation: add about 9000ml absolute ethanol to the above-mentioned concentrated solution, adjust to 80% ethanol c...

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Abstract

The invention provides a preparation method of the polysaccharide extract of Dendrobium nobile. The polysaccharide extract of Dendrobium nobile is achieved through the steps of degreasing, extraction, alcohol precipitation, protein removal, monosaccharide and oligosaccharide removal, and gel column purification. The polysaccharide content in the polysaccharide extract is above 93%. The polysaccharide extract has significant antioxidant activity and can be used in the preparation of medicines or pharmaceutical compositions for treating and preventing cardiovascular diseases in combination with pharmaceutical excipients or carriers allowed by preparations. The preparation method of the extract of the invention is reasonable in design and easy to operate.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to a preparation method and application of a polysaccharide extract of Dendrobium nobile. Background technique [0002] Dendrobium is a commonly used famous and precious Chinese medicinal material, which is the collective name for the fresh or dried stems of various plants of the genus Dendrobium Sw. in the family Orchidaceae. Dendrobium is a large genus in the Orchidaceae, with about 1100 species in the world, and about 76 species in my country. Dendrobium was first recorded in "Shen Nong's Materia Medica", and was listed as a top grade. It has the effects of nourishing yin and clearing heat, nourishing the stomach and promoting body fluid, moistening the lungs and relieving cough. The 2005 edition of "Chinese Pharmacopoeia" recorded three kinds of Dendrobium nobile Lindl., Dendrobium officinale Kimura et Migo and Dendrobium versicolor (D. fimbriatum Hook.). [0003] Mode...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P35/00C08B37/00A61K31/715A61P37/04A61K36/8984A61P9/00A61P3/10
Inventor 程翼宇吴斌姚宏
Owner ZHEJIANG UNIV
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