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Claret Chinese cabbage breeding method

A technology of purple and Chinese cabbage, applied in the field of cruciferous vegetable breeding, can solve the problem of no purple Chinese cabbage resources, and achieve the effect of rich varieties and high nutritional value

Inactive Publication Date: 2011-04-27
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are purple cabbage, laver sprouts, purple mustard greens, etc. among the Brassica vegetables, but there are no resources of purple Chinese cabbage.

Method used

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  • Claret Chinese cabbage breeding method
  • Claret Chinese cabbage breeding method
  • Claret Chinese cabbage breeding method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043]Select the purple mustard local variety Hongyeqingcai in Qianyang, Hunan (quoted from the National Variety Resources Medium-term Preservation Bank of the Institute of Vegetable and Flower Research, Chinese Academy of Agricultural Sciences, introduction number: II3A0138), through continuous selfing of individual plants through optimized plants, selfing 3-5 generations, The self-bred seeds of Mustard mustard with no trait segregation in offspring and purple-red stems and leaves were selected to obtain a genetically stable homozygous inbred line PM058.

[0044] Preparation of B5 extraction medium: According to the B5 medium described in Gamborg, O.L., et al., 1968, Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151-158., first prepare macronutrients (concentrated 20 times), trace elements (concentrated 200 times), iron salts (concentrated 200 times) and organic substances other than sucrose (concentrated 200 times) concentrated stock s...

Embodiment 2

[0053] Configure B5 solid medium: according to the B5 medium described in the literature Gamborg, O.L., et al., 1968, Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151-158. First prepare macronutrients (concentrated 20 times), trace elements (concentrated 200 times), iron salts (concentrated 200 times) and organic substances other than sucrose (concentrated 200 times) concentrated stock solution, then take a 1L volumetric flask, take macroelement concentrated stock solution 50ml, 5ml each of trace elements, iron salts, and concentrated stock solutions of the above-mentioned organic substances, and the following substances were added to make the concentration 6-benzylaminopurine (6-BA) 1.5mg·L -1 , α-naphthaleneacetic acid (NAA) 0.05mg·L -1 , sucrose 30.0g·L -1 , agar 7.0g·L -1 , distilled water to volume, then adjust the pH value of the solution to 5.8-6.0, and distribute it in 100ml Erlenmeyer flasks. After autoclaving (121°C, 15min...

Embodiment 3

[0065] F1 plants were obtained through distant hybridization with purple mustard PM058 as the female parent and Chinese cabbage DH line Juhongxin C24 as the male parent;

[0066] The obtained fuchsia F1 plant was used as the female parent, and the DH line of Chinese cabbage, Juhongxin C24, was used as the male parent to backcross to obtain (Juhongxin C24) BC1 seeds;

[0067] The obtained (Juhongxin C24) BC1 seeds were identified in the field, the purple-red plant was selected as the female parent, and the Chinese cabbage DH line Juhongxin C24 was used as the male parent to continue backcrossing to obtain (Juhongxin C24) BC2 seeds;

[0068] The obtained (Juhongxin C24) BC2 seeds were identified in the field, the purple-red plant was selected as the female parent, and the Chinese cabbage DH line Juhongxin C24 was used as the male parent to continue backcrossing. Through field selection and continuous backcrossing, normal flowering and seed formation were obtained. Normal fuchsia...

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Abstract

The invention relates to a selection and cultivation method of purplish red cabbage. Plant generation F1 whose male parent is cabbage and female parent is purplish red rutabaga is cultivated by distant hybridization; the backcross of the hybrid plants takes cabbage as male parent; cabbage with stable hereditary performance and trait of purplish red is obtained by self-crossing purplish red plants whose general traits are similar to the traits of cabbage after generations of backcross. Different obvolvent types such as obvolvent, unrolling carpel, straight, overlapping-folded and conjunctionally folded can be obtained by the method according to the hereditary performance from male parent, meanwhile the growth season can be controlled in the same way. The invention enriches the breed variety of cabbage and leads a novel direction of breeding cabbage and improving the quality and nutritive value thereof.

Description

technical field [0001] The invention relates to the technical field of cruciferous vegetable breeding, in particular to a method for breeding purple-red Chinese cabbage. Background technique [0002] Chinese cabbage (Brassica rapa L.ssp.pekinensis) is native to China. It is an annual and biennial herb of the genus Brassica in the Brassicaceae family. One of the crops, it is still the staple vegetable with the widest distribution, the largest cultivation area, and the largest number of people in my country. [0003] With the continuous improvement of people's living standards, the quality breeding of Chinese cabbage has been paid more and more attention by many breeders. In recent years, Chinese cabbage with orange red heart and Chinese cabbage with yellow heart, which have been bred successively at home and abroad, are favored by consumers. Breeding with different ball colors has become a new direction for the selection of new, odd and extra-large Chinese cabbage varieties,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04A01H1/02A01H5/12
Inventor 孙日飞李菲张淑江章时蕃
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI