Method for improving arpagoside extraction rate in radix scrophulariae by fermentation method
A technology of harpagoside and extraction rate in ginseng, which is applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of complex bacterial strains, difficulty in directional transformation of key components, long transformation cycle, etc., and achieve the goal of cultivating Ease, ease of biotransformation, good method and the effect of the platform
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Embodiment 1~3
[0024] Experiments on Transformation Effects of Different Strains of Scrophulariaceae
[0025] See attached figure 1 Scrophulariae scrophulariae was transformed using alcoholic yeast, Meishan yeast and brewer's yeast as strains, and harpagoside was extracted and determined for untransformed and transformed Scrophulariae scrophulariae. The conditions and results were as follows:
[0026] Each strain was stored on a slant in wort solid medium, activated at 28°C for 24 hours, and then inoculated on wort liquid medium (no agar, pH 5.5). The liquid seeds were cultured in a 250 ml shaker bottle with 50 ml liquid, the fermentation temperature was 28° C., the shaker speed was 150 r / min, and cultured for 24 hours to obtain a culture solution.
[0027] Accurately weigh 2 grams of Scrophulariaceae that has been crushed to 20-60 meshes, dissolve it in 50 ml of distilled water, adjust the pH to 5.5, and sterilize at 121°C for 15 minutes to obtain Scrophulariaceae culture medium; Insert 1...
Embodiment 4~6
[0033] Experiments on Transformation Effects of Scrophulariaceae with Different pH
[0034] According to the method of Example 1, only Scrophulariae cerevisiae was used as the strain to transform Scrophulariaceae culture medium with different pH, and the transformed Scrophulariaceae was extracted and its content was determined. The results are shown in Table 2:
[0035] Table 2
[0036]
[0037] The comparative analysis in Table 2 found that the use of different pH mediums of Scrophulariae scrophulariae significantly affected the content of harpagoside in Scrophulariae scrophulariae, and the yield of harpagoside was the highest at pH=5.5.
Embodiment 7~10
[0039] Experiments on Transformation Effects of Different Inoculation Ratio on Scrophulariaceae Scrophulariae
[0040]The cells were obtained by culturing according to the method of Example 1. Saccharomyces cerevisiae was used as the transformed microorganism, and the culture solution was inserted into the culture medium of Scrophulariaceae Scrophulariae according to different inoculation ratios for transformation. The content of harpagoside was determined for the transformed Scrophulariaceae Scrophulariae. The results are shown in table 3:
[0041] table 3
[0042]
[0043] Note: Each ml of culture medium contains 1×10 strain cells 8 cfu.
[0044] It can be seen from Table 3 that the inoculation ratio of 1:1 is very effective for the transformation of Scrophulariaceae, and the yield of harpagoside is significantly higher than that of other groups. Therefore, it is more beneficial to select the inoculation ratio of 1:1 for the transformation of Scrophulariaceae. Extracti...
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