Compositional main pesticide and pesticide containing destruxins of Metarhizium anisopliae and high-efficient cypermethrin

Abstract

The invention discloses a pesticidal main agent and a pesticide by compounding destruxins of Metarrhizium anisopliae and high-efficiency cypermethrin. The mass ratio of the destruxins of Metarrhizium anisopliae to the high-efficiency cypermethrin in the main agent is 7-1:3-9. The invention adopts pyrethroid pesticide with high efficiency, low toxicity, low residue and broad spectrum activity, namely the high-efficiency cypermethrin, and entomopathogenic fungi with obvious disinsection effect, namely the destruxins of Metarrhizium anisopliae, for compounding to obtain the pesticidal main agent, and determines the preferable compounding ratio of both through a large number of experiments. The pesticide has brand-new action mechanism different from the prior pesticides and obvious disinsection effect, particularly has obvious toxic action on prodenia litura which has severe harm, and has the characteristic of environmental protection at the same time.

Description

technical field

[0001] The invention belongs to the technical field of biopesticides, and relates to an insecticide main agent and an insecticide compounded with metarhizine and beta-cypermethrin. Background technique

[0002] Spodoptera litura is an important omnivorous agricultural pest distributed worldwide. In recent years, the frequency of occurrence of large-scale pests in many areas of my country has increased significantly, and the damage has aggravated. Due to the long-term use of chemical pesticides, the insect has developed resistance to organochlorines, organophosphorus, carbamates, pyrethroids and other insecticides to varying degrees. Its harm is difficult to control. High-beta-cypermethrin is one of the important agents for controlling Spodoptera litura. Due to long-term use, it has developed drug resistance, so it is urgent to develop effective compound agents.

[0003] Beta-cypermethrin (alpha-Cypermethrin, high chlorine) is currently widely used high-effi...

Examples

Embodiment 1

[0018] Example 1 Indoor Toxicity Determination Experiment of Metarhizin and Beta-cypermethrin Compound Main Agent of the Present Invention on Spodoptera litura

[0019] During the indoor toxicity test, according to the mass ratio of metarhizine and beta-cypermethrin is 7 ~ 1: 3 ~ 9, measure the appropriate amount of medicine respectively, make a mother solution with a certain concentration with acetone and put it in the refrigerator (4 ℃) for later use. The medicines were all diluted with 0.01% Tween-80 solution. The metarhizine is any one of metarhizin A, metarhizin B or metarhizin E, or a mixture of two or more in any proportion. Each treatment was replicated 3 times, and each replicate had 1 petri dish, and 15 third-instar Spodoptera litura larvae were placed in each petri dish. First, cut the leaves of cabbage into small pieces of 6-7 cm, soak them in the medicinal solution for about 5 seconds, take them out, dry them indoors, put the leaves into a petri dish connected wi...

Embodiment 2

[0024] Example 2 20% of the compound insecticide of the present invention (the mass ratio of metarhizine: beta-cypermethrin=3:7) EC is tested for indoor toxicity of Spodoptera litura

[0025] The above-mentioned emulsifiable concentrate was diluted with water to the concentration gradient to be tested, and the experimental method was the same as in Example 1. The experimental results are shown in Table 2, and its co-toxicity coefficient reaches 179.52, which has obvious synergistic effect.

[0026] Table 2 Toxicity determination results of metarhizalin, beta-cypermethrin single agent and emulsifiable concentrate of the present invention to 3rd instar larvae of Spodoptera litura

[0027]

technical field

[0001] The invention belongs to the technical field of biopesticides, and relates to an insecticide main agent and an insecticide compounded with metarhizine and beta-cypermethrin. Background technique

[0002] Spodoptera litura is an important omnivorous agricultural pest distributed worldwide. In recent years, the frequency of occurrence of large-scale pests in many areas of my country has increased significantly, and the damage has aggravated. Due to the long-term use of chemical pesticides, the insect has developed resistance to organochlorines, organophosphorus, carbamates, pyrethroids and other insecticides to varying degrees. Its harm is difficult to control. High-beta-cypermethrin is one of the important agents for controlling Spodoptera litura. Due to long-term use, it has developed drug resistance, so it is urgent to develop effective compound agents.

[0003] Beta-cypermethrin (alpha-Cypermethrin, high chlorine) is currently widely used high-effi...

Examples

Embodiment 1

[0018] Example 1 Indoor Toxicity Determination Experiment of Metarhizin and Beta-cypermethrin Compound Main Agent of the Present Invention on Spodoptera litura

[0019] During the indoor toxicity test, according to the mass ratio of metarhizine and beta-cypermethrin is 7 ~ 1: 3 ~ 9, measure the appropriate amount of medicine respectively, make a mother solution with a certain concentration with acetone and put it in the refrigerator (4 ℃) for later use. The medicines were all diluted with 0.01% Tween-80 solution. The metarhizine is any one of metarhizin A, metarhizin B or metarhizin E, or a mixture of two or more in any proportion. Each treatment was replicated 3 times, and each replicate had 1 petri dish, and 15 third-instar Spodoptera litura larvae were placed in each petri dish. First, cut the leaves of cabbage into small pieces of 6-7 cm, soak them in the medicinal solution for about 5 seconds, take them out, dry them indoors, put the leaves into a petri dish connected wi...

Embodiment 2

[0024] Example 2 20% of the compound insecticide of the present invention (the mass ratio of metarhizine: beta-cypermethrin=3:7) EC is tested for indoor toxicity of Spodoptera litura

[0025] The above-mentioned emulsifiable concentrate was diluted with water to the concentration gradient to be tested, and the experimental method was the same as in Example 1. The experimental results are shown in Table 2, and its co-toxicity coefficient reaches 179.52, which has obvious synergistic effect.

[0026] Table 2 Toxicity determination results of metarhizalin, beta-cypermethrin single agent and emulsifiable concentrate of the present invention to 3rd instar larvae of Spodoptera litura

[0027]

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