Vancomycin-resistant Staphylococcus aureus medium

A vancomycin and staphylococcus technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of no vancomycin-resistant Staphylococcus aureus chromogenic medium, etc., and achieve operability. strong effect

Active Publication Date: 2011-12-14
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the chromogenic medium for methicillin-resistant S. aureus has appeared on the market, there is no chromogenic medium suitable for the identification of vancomycin-resistant S. aureus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Each 1000g medium contains: agar 10g, tryptone 18g, yeast powder 7g, sodium pyruvate 11g, lithium chloride 8g, potassium tellurite 5mg, vancomycin 24mg, 5-bromo-4-chloro-3- Indole phosphate 1.5g, tetrazolium blue 0.7g, the balance is water. The above raw materials are evenly mixed and heated to 100-110°C, heated for 10 minutes, and filled in a petri dish.

Embodiment 2

[0017] Each 1000g medium contains: agar 13g, tryptone 14g, yeast powder 2g, sodium pyruvate 8g, lithium chloride 2g, potassium tellurite 8mg, vancomycin 7mg, 5-bromo-4-chloro-3- Indole phosphate 1g, tetrazolium blue 0.3g, and the balance is water. After the above-mentioned raw materials are evenly mixed, the temperature is raised to 100-110°C, heated for 15 minutes, and then filled in a petri dish.

Embodiment 3

[0019] Each 1000g medium contains: 15g agar, 16g tryptone, 4g yeast powder, sodium pyruvate 5g, lithium chloride 6g, potassium tellurite 3mg, vancomycin 15mg, 5-bromo-4-chloro-3- Indole phosphate 1.8g, tetrazolium blue 0.5g, the balance is water. After the above-mentioned raw materials are evenly mixed, the temperature is raised to 100-110°C, heated for 15 minutes, and then filled in a petri dish.

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Abstract

The invention belongs to the field of in vitro diagnostic reagents, in particular to a culture medium for identifying vancomycin-resistant Staphylococcus aureus. The culture medium of the invention not only contains nutrients, coagulation substances and miscellaneous bacteria inhibitors, but also contains vancomycin and color indicator 5-bromo-4-chloro-3-indolyl-phosphate and nitrogen blue tetrazolium. Bacteria inhibitors can inhibit the growth of bacteria other than Staphylococcus aureus, and vancomycin can inhibit the growth of Staphylococcus aureus that is sensitive to it. The indicator can make the vancomycin-resistant Staphylococcus aureus colony appear purple, further distinguishing it from other drug-resistant bacteria. The present invention combines the layer-by-layer screening culture of vancomycin-resistant Staphylococcus aureus and the detection of its characteristic enzymes, can accurately and visually detect and identify vancomycin-resistant Staphylococcus aureus, has strong operability, and is suitable for Promoted in hospitals and other research institutions, it will help guide doctors' medication and the development of new antibiotics.

Description

Technical field [0001] The invention belongs to the field of in vitro diagnostic reagents, and specifically relates to a culture medium resistant to vancomycin Staphylococcus aureus. Background technique [0002] In our country, the abuse of antibiotics is very common. After the bacteria have been in contact with the drug for many times, the sensitivity to the drug decreases or even disappears, resulting in the lower or even ineffective efficacy of the drug against the drug. Staphylococcus aureus (abbreviated as Staphylococcus aureus) has defeated natural penicillin, ampicillin, cephalosporin, etc. successively in the "encounter" with antibiotics, and even the emergence of Staphylococcus aureus resistant to vancomycin ( vancomycin-resistant staphylococcus aureus, abbreviated as VRSA). The continuous escalation of bacterial resistance has almost forced doctors into an embarrassing situation where no medicine is available. The emergence of many drug-resistant bacteria has become...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/14
Inventor 罗江卫王则宇康运凯冯长城崔晓晓杨红云郑业焕付光宇吴学纬
Owner AUTOBIO DIAGNOSTICS CO LTD
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