Method for breeding heavy metal hyperaccumulative plant Sedum alfredii Hance germchit

A technology of hyperaccumulation and heavy metals, which is applied in the field of reproduction of heavy metal hyperaccumulation plant Sedum sedum sedum seedlings, can solve problems such as no research report on seedling reproduction technology, and achieves fast propagation method, high transplant survival rate, and easy operation. Effect

Active Publication Date: 2011-06-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is a wild plant, and its seedling propagation technology has never been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Cut Sedum sedum from the lead-zinc mine overaccumulated with heavy metals into about 4cm stems with axillary buds, and cut them directly into 1 mu of heavy metal-polluted vegetable fields in Shanghai, cover them with shade nets for 4 days, and water every 2 days in the morning , remove the sunshade net after the cutting stems stand upright, water twice a week to keep the soil moist, weeding, after 120 days of growth, the plant grows to about 20cm, and has many branches, the branch length is about 8cm, cut off the ground After planting, keep the plants containing about 2cm shoots in the soil, water 2 times a week, add urea and rapeseed cake, keep the plants growing vigorously, and harvest again after 120 days. The above-ground stem segment obtained by harvesting is cut into again as a seedling and cut into about 4 cm stem segment with axillary buds and propagated in polluted soil.

Embodiment 2

[0027] Cut Sedum sedum from the lead-zinc mine overaccumulated with heavy metals into about 4cm stems with axillary buds, insert the cuttings into a substrate with a ratio of 3:1 of vermiculite and perlite washed with water, cover the sunshade net for one week, and Water every 3 days, remove the sunshade net after the cutting stems stand upright, water 2 times a week to keep the substrate moist, after about 1 month of growth, get about 10cm thick Sedum sedum seedlings, transplant to Fuyang grows in soil heavily polluted by heavy metals, weeds, grows for about 4 months, harvests the aboveground part, keeps the plants with about 5cm aboveground part in the soil, conducts daily field management, and harvests again after half a year.

Embodiment 3

[0029]Cut the Sedum sedum from the lead-zinc ore over-accumulation of heavy metals into about 3cm stems with axillary buds, insert them into the water, grow about 1cm of young roots after 1 week, and replace them with 1 / 4 concentration of nutrients with a pH of 5.8 Liquid (the formula is: Ca(NO 3 ) 2 4H 2 O 2.00mmol L -1 , KH 2 PO 4 0.10mmolL -1 , MgSO 4 ·7H 2 O 0.50mmolL -1 , KCl 0.10mmol L -1 , K 2 SO 4 0.70mmolL -1 , H 3 BO 3 10.00 μmol L -1 , MnSO4·H 2 O0.50 μmol L -1 , ZnSO 4 ·7H 2 O 1.0 μmol L -1 , CuSO 4 ·5H 2 O 0.20 μmol L -1 , (NH 4 ) 6 Mo 7 o 24 0.01 μmol L -1 , Fe-EDTA 100 μmol L -1 ) cultured for 3 days, replaced with 1 / 2 concentration of nutrient solution (same formula as above) with a pH of 5.8 for 3 days, and then replaced with a nutrient solution (same formula as above) with a full concentration of pH 5.8 for cultivation, and replaced every 3 to 4 days A nutrient solution, cultivated for 15 days to obtain seedlings with a small a...

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Abstract

The invention relates to a method for breeding heavy metal hyperaccumulative plant Sedum alfredii Hance germchit. In the method, a stem section, a stem tip or a lamina of the heavy metal hyperaccumulative plant Sedum alfredii Hance is cultivated and bred. The method not only has a wide range for selecting materials, but each step during the cultivation and breeding is also easy for operating and can be monitored; transplant survival rate is high; the breeding method has fast speed and high efficiency; and the method provides condition for restoring large area of land with heavy metal pollution.

Description

[0001] This application is a divisional application with the application number: 200610155240.4, the filing date: 2006.12.15, and the title of the invention is "propagation method of heavy metal hyperaccumulation plant Sedum sedatus seedlings". technical field [0002] The invention relates to a propagation method of the heavy metal hyperaccumulation plant sedum sedum seedlings. Background technique [0003] With the development of industry and the modernization of agricultural production, the problem of heavy metal pollution in the soil-plant-environment system is becoming more and more serious. Heavy metal pollution not only reduces the yield and quality of crops, leads to the degradation of soil function and quality, and affects the healthy and sustainable development of agricultural production, but also pollutes surface water and groundwater through runoff and leaching, deteriorates the hydrological environment, and may cause pollution through direct contact, food chains,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00C12N5/04
Inventor 杨肖娥金晓芬冯英吴春勇刘勇军
Owner ZHEJIANG UNIV
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