Fast seedling-breeding method of samphire of North American by issue culture

A technology of tissue culture and Salicornia, which is applied in the field of rapid propagation of Salicornia seedlings through tissue culture in North America, can solve the problems of slow growth, low survival rate, and difficult management of Salicornia seedlings, and achieve increased reproduction speed and transplantation. The effect of planting survival rate and widening the scope of materials

Inactive Publication Date: 2011-01-12
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth of Salicornia seedlings is extremely slow, management is difficult, and the survival rate is low

Method used

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  • Fast seedling-breeding method of samphire of North American by issue culture
  • Fast seedling-breeding method of samphire of North American by issue culture
  • Fast seedling-breeding method of samphire of North American by issue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The germination medium is MS+TDZ 1.0mg / L+NaCl 8.0g / L.

[0019] Rinse the seeds of samphire with tap water to remove the floating stunted seeds, place them in a 1-liter beaker, soak them in a solution of 75% alcohol for 1 minute, rinse them once with sterile water, and then rinse them with mercuric chloride Disinfect with 0.1% surface disinfectant for 20 minutes and wash with sterile water six times. Inoculate on germination medium and culture in dark conditions. Germination began 3 days after inoculation, 4-5 clustered plantlets could be formed in about January, and 15-20 clustered plantlets could be formed in about February. Can turn green and form robust clusters of plantlets.

Embodiment 2

[0021] Proliferation medium is MS+TDZ 1.0mg / L+BA 2.0mg / L+NaCl 8.0mg / L.

[0022] Get the stem tip of the strong clustered plantlet that embodiment 1 obtains, and the stem of the clustered plantlet is cut into 1.2~1.8cm size stem segments and inoculated on the proliferation medium, make the shoot tip and the small stem segment in the proliferation medium Rapidly subcultured and multiplied to form clustered young seedlings of Salicornia saponica, and the monthly multiplication multiple can reach more than 1.5 times that of the tissue culture seedling propagation system of Salicornia established by CW Lee.

Embodiment 3

[0024] The rooting medium is 1 / 2MS+IBA 0.3mg / L+KT 0.1mg / L+activated carbon 0.5mg / L+malt extract 0.5mg / L+NaCl 8.0mg / L.

[0025] Seedlings higher than 2 cm after the propagation of Example 2 were inoculated on the rooting medium, and adventitious roots began to germinate in about 7 days, and the average number of roots per plant could reach 3 to 6 in about 20 days, forming the Salicornia seedlings.

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Abstract

The invention relates to a fast seedling-breeding method of samphire of North American by tissue culture. The used culture medium for germination and gemmulation is MS+1.0mg / L of TDZ+ 8.0g / L of NaCl; proliferative culture medium is MS+ 1.0mg / L of TDZ + 2.0mg / L of BA + 8.0g / L of NaCl; rootage culture medium is 1 / 2 of MS+0.3mg / L of IBA + 0.1mg / L of KT + 0.5mg / L of active carbon + 0.5mg / L of malt extract + 8.0g / L of NaCl; the culture processes are as follows: the seeds of the samphire of North American is inoculated on the culture medium for germination and gemmulation to germinate strong tuft plantlet; the stem tip and the stem section cut from the tuft plantlet are arranged on the proliferative culture medium and the rootage culture medium for secondary proliferation and rootage; seedlingswith three to six roots are transplanted in matrix prepared by river sand, peaty soil and garden soil according to the volume proportion of 1:1:1; and the survival seedlings are transplanted and fixedly planted in large fields.

Description

technical field [0001] The invention relates to a seedling propagation method of Salicornia salicornia, in particular to a rapid seedling propagation method of Salicornia salicornia through tissue culture. technical background [0002] Salicornia Bigelovii Torr., also referred to as Salicornia, belongs to Chenopodiaceae (Chenopodiaceae), Salicornia (Salicornia), also translated as Salicornia, is a true species with strong salt tolerance. Halophytes. It can be directly irrigated with seawater, and can be planted on coastal beaches. Salt tolerance limit reaches 5% NaCl concentration. Salicornia is the most economically valuable euhalophyte selected from more than 1,300 wild halophytes collected by the Environmental Research Office of the University of Arizona in the United States. It can be used as pollution-free vegetables, animal green feed and bioenergy Material cultivation application. Cultivated as a vegetable, its young stems, leaves and seed pods are picked, stir-fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 余桂红柏文娟马鸿翔周淼平张旭孙晓波任丽娟
Owner JIANGSU ACAD OF AGRI SCI
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