Jujube leaf stalk culture direct becoming bud method and novel culture medium thereof

Abstract

The invention relates to a method of directly culturing the stipe of jujube tree into sprout and the new culture medium. The invention selects the stipe of the reciprocal third or fourth leaf from the top down of the tissue culture seedling of jujube tree as explant or selects the tip tender stipe on healthy jujube tree as the explant when non-tissue culture seedling, wherein, the tender stipe isthe new stipe growing during the present month or the last month. The selected explant is then inoculated in new culture medium and cultured on a culturing frame. The adventitious bud can be split apart directly by be culturing for about 40 days under the conditions of the culturing room that the temperature during night is 23 DEG C with plus or minus 1 DEG C, the temperature during day is 27 DEGC with plus or minus 1 DEG C, the relative humidity is 60 to 70 percent, the illumination intensity is 1500 to 2000 LX and the illumination hourage is 12 h / d. The invention adopts the stipe of jujubetree as the material and can directly induce the adventitious bud by one-step culturing method in the new culture medium, wherein, the inductivity can reach about 40 percent. The invention can expandthe explant material range of the tissue culture of jujube tree and also provides a new technological approach for rapid reproducing and breeding of the jujube tree.

Description

technical field [0001] The invention relates to a method for direct bud formation by cultivating jujube petioles and a new culture medium thereof, and is especially suitable for rapid propagation and breeding of jujube trees. Background technique [0002] At present, the known tissue culture of jujube is to carry out rapid propagation with jujube leaves or stem segments and stem tips as explants, and to induce adventitious buds with leaves as explants. These two methods are different except for explants. The well-known MS (majority) or WPM (minority) is the basic medium. The former needs to undergo primary culture and subculture to form adventitious buds, and the latter needs to undergo dedifferentiation culture to form callus, and then through callus differentiation culture to induce Adventitious buds. Obviously, due to the difference in the selection of explants and medium, and the need for at least two-step culture, that is, first use one medium to induce callus through ...

AI Technical Summary

Problems solved by technology

That is to say, the culture medium needs to be replaced and cultured in two times, which results in the limitation of jujube tissue culture in the selection of explants, as well as the cumbersome and low induction rate of jujube leaf culture regeneration plant culture procedures, thereby limiting the jujube rapid growth rate. Breeding progress

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