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Method for extracting anti-tumor effective component of Prunella vulgaris and application of extract thereof in preparing anti-tumor medicament

A technology of effective parts and extraction methods, applied in the field of extracting effective parts of Prunella vulgaris for anti-tumor, can solve the problem of no experimental research report on anti-malignant lymphoma, and achieve no obvious toxic and side effects, improve therapeutic effect, and affirmative anti-tumor effect. Effect

Active Publication Date: 2009-06-24
张明智 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the literature search and patent search, there is no experimental research report on the effective extraction parts of Prunella vulgaris against malignant lymphoma

Method used

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  • Method for extracting anti-tumor effective component of Prunella vulgaris and application of extract thereof in preparing anti-tumor medicament
  • Method for extracting anti-tumor effective component of Prunella vulgaris and application of extract thereof in preparing anti-tumor medicament
  • Method for extracting anti-tumor effective component of Prunella vulgaris and application of extract thereof in preparing anti-tumor medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Extraction step

[0017] a. Prunella vulgaris flower ears collected at the end of June and early July in Queshan County, Henan Province, soaked in 95% ethanol in a multi-functional extractor, heated and refluxed for 5 hours after 30 hours to obtain the extract, and concentrated the extract under reduced pressure to obtain the extract Part of the obtained extract was subjected to in vitro tumor cell inhibition rate experiments, and the other part was extracted in the next step.

[0018] b. Concentrate the extract under reduced pressure to dryness to obtain the extract, extract the obtained extract with 1:1 ethyl acetate and water, collect the organic phase and the aqueous phase respectively, concentrate to dryness under reduced pressure, and carry out tumor cell inhibition in vitro rate experiment.

[0019] c. According to the experimental results of tumor cell inhibition rate in vitro, take the organic phase and dissolve it with chloroform, methanol, and water with ...

Embodiment 2

[0032] 1. Extraction step

[0033] a. Prunella vulgaris flower ears collected at the end of June and the beginning of July in Queshan County, Henan Province, soaked in 85% ethanol in a multi-functional extractor, heated and refluxed for 3 hours after 24 hours to obtain the extract, and concentrated the extract under reduced pressure to obtain the extract Part of the obtained extract was subjected to in vitro tumor cell inhibition rate experiments, and the other part was extracted in the next step.

[0034] b. Concentrate the extract under reduced pressure to dryness, extract the obtained extract with 1:0.5 chloroform and water, collect the organic phase and the aqueous phase respectively, concentrate to dryness under reduced pressure, and carry out an in vitro tumor cell inhibition rate experiment .

[0035] c. According to the experimental results of tumor cell inhibition rate in vitro, take the organic phase and dissolve it with chloroform, methanol, and water with a volume...

Embodiment 3

[0049] 1. Extraction step

[0050] a. Prunella vulgaris flower ears collected at the end of June and the beginning of July in Queshan County, Henan Province, soaked in 96% ethanol in a multifunctional extractor, heated and refluxed for 6 hours after 36 hours to obtain the extract, and concentrated the extract under reduced pressure to obtain the extract Part of the obtained extract was subjected to in vitro tumor cell inhibition rate experiments, and the other part was extracted in the next step.

[0051] b. Concentrate the extract under reduced pressure to dryness to obtain the extract, extract the obtained extract with 1:1.2 ethyl acetate and water, collect the organic phase and the aqueous phase respectively, concentrate to dryness under reduced pressure, and carry out tumor cell inhibition in vitro rate experiment.

[0052] c. According to the experimental results of tumor cell inhibition rate in vitro, take the organic phase and dissolve it with chloroform, methanol, and...

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Abstract

The invention relates to a method for extracting antitumor effective sites of selfheal, which comprises: using ethanol to soak spica of the selfheal in an extractor, and performing heating, reflux and extraction; concentrating an extracting solution into a dried extract, using chloroform and water or ethyl acetate and the water to extract the dried extract, respectively collecting an organic phase and an aqueous phase, and concentrating the organic phase and the aqueous phase to be dried; using the chloroform, methanol and the water to dissolve the organic phase, separating out superstratum after standing, and concentrating an upper solution to be dried; and adding a mobile phase to dissolve upper residue, performing reversed-phase column chromatography and elution, collecting 30 to 80 percent methanol eluent, concentrating eluent of various phases to be dried, and obtaining the antitumor effective sites. The in vitro experiments of the effective sites of the selfheal determined by the proposal of the method can obviously inhibit proliferation of Rajicells, Jurkat cells, K562 cells and 435S cells; and the in vivo experiments have tumor inhibitory efficacy on a He T lymphoma EL-4 cell mouse, and can prolong the life cycle of the He T lymphoma EL-4 cell mouse; and the antitumor effective sites of the selfheal have definite antitumor function and low toxicity.

Description

technical field [0001] The invention relates to a method for extracting effective anti-tumor parts of Prunella vulgaris and its effect on anti-tumor. Background technique [0002] Prunella vulgaris is the dry and mature fruit ear of the Lamiaceae plant Prunella vulgaris L, mainly produced in Queshan County, Henan Province and other places. Pain and other diseases. Prunella has a long history in the treatment of malignant lymphoma, and many remedies and prescriptions use Prunella as the main drug for the treatment of malignant lymphoma. According to the clinical observation of the compound traditional Chinese medicine preparation with Prunella vulgaris as the main medicine in the treatment of malignant lymphoma for many years, the applicant of the present patent found that it can make the tumor of malignant lymphoma regress. Modern pharmacological studies have shown that Prunella vulgaris decoction can induce apoptosis of SGC-7901 cancer cells in vitro, and has inhibitory e...

Claims

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Application Information

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IPC IPC(8): A61K36/536A61P35/00A61K133/00
Inventor 张明智刘宏民
Owner 张明智
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