Raising method of phyllotreta striolata experimental population

Abstract

The invention discloses a raising method of phyllotreta striolata experimental population, aiming at providing a raising method of the phyllotreta striolata experimental population which has almost no damage to ovum and larva, has high hatchability, high livability and each accurate worm state and uniform age. The technical proposal of the invention is that: vegetable leaves are used for feeding adults, so as to lead the adults to lay on a black cloth and the vegetable leaves, and then radish skin is used for feeding larva until the larva is maturated, and the maturated larva is placed in 1 percent to 1.5 percent of wet sands and the eclosion of the maturated larva is carried out and the adults are formed. The raising method is mainly used for providing enough experimental population for finishing deep system research in the technical field such as biology, ecology and prevention and the like of the phyllotreta striolata.

Description

Technical field: [0001] The invention belongs to the field of biological technology, and in particular relates to a method for raising experimental populations of flea beetle flea beetle. Background technique: [0002] Phyllotreta striolata (Fabricius) belongs to Coleoptera, Chrysophyllidae, also known as dog lice, vegetable fleas, soil fleas, yellow fleas, is a worldwide pest that mainly damages cruciferous vegetables, and is found in Asia, Europe, North It is distributed in America and South Africa, and almost all provinces in China. [0003] The adult flea beetle bites leaves and forms a large number of holes, which affects the quality and commercial value of vegetables; the cotyledons of seedlings are eaten by adults, and the whole plant dies, resulting in lack of seedlings, and even reseeding is required in severe cases. The larvae feed on the roots, peel off the vegetable root skin, and cause the plants to wither and die; when the radish tubers are damaged, many dark ...

AI Technical Summary

Problems solved by technology

First, adults lay their eggs on the roots, stems or petiole bases of seedlings, or on filter paper, so they are vulnerable to damage when picking eggs, reducing the hatching rate of eggs

Secondly, when checking the development and survival of larvae, it is necessary to untie the filter paper and black cloth and then peel off the root epidermis of the plant to search. The young larvae are small and the color is very close to the root epidermis. It is not only time-consuming and laborious to find, but also easy to get rid of. Dead larvae; the highest survival rate in the immature stage of raising the flea beetle with the "whole seedling large bottle covering method" recorded in the existing bibliographical reports is 63.3% (a new method for raising the flea beetle experimental population, Liu Yun, You Minsheng, Hou Youming, Knowledge of Insects, Vol. 42, No. 5, pp. 578-581, 2005); in addition, since the larvae cannot be observed directly, it is impossible to obtain accurate information on the larvae of each instar

Furthermore, the "whole seedling large bottle cage method" has many materials and complicated procedures, and requires a large space to place plastic bottles during large-scale breeding, which is not suitable for large-scale breeding in laboratories.