Application of recombinant FN heparin binding domain polypeptide on preparing medicament resisting invasion and metastasis of malignant tumor

A technology of heparin binding domain and polypeptide, applied in the field of medicine, to achieve the effect of promoting angiogenesis, enhancing cell adhesion and proliferation, and enhancing activity

Active Publication Date: 2013-03-27
FUJIAN MEDICAL UNIV UNION HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of recombinant FN N-terminal and C-terminal heparin-binding domain peptides on the invasion and metastasis of malignant tumors has never been reported

Method used

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  • Application of recombinant FN heparin binding domain polypeptide on preparing medicament resisting invasion and metastasis of malignant tumor
  • Application of recombinant FN heparin binding domain polypeptide on preparing medicament resisting invasion and metastasis of malignant tumor
  • Application of recombinant FN heparin binding domain polypeptide on preparing medicament resisting invasion and metastasis of malignant tumor

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Embodiment Construction

[0045] The present invention is described in detail below in conjunction with accompanying drawing and embodiment:

[0046] The injection of mouse melanoma cell B16 cells into mice is used as an example below to illustrate that the recombinant FN heparin-binding domain polypeptide can be used in the preparation of drugs against invasion and metastasis of malignant tumors.

[0047] (1) Materials and reagents

[0048] 1. Main reagents

[0049] Native full-length fibronectin (FN): Purified from normal human plasma by gelatin affinity chromatography.

[0050] Recombinant FN polypeptide: recombinant FN N-terminal heparin-binding domain polypeptide (rhFNHN-29), recombinant FN C-terminal heparin-binding domain polypeptide (rhFNHC-36): the present invention constructs two heparin-binding domain polypeptides of FN N-terminus and C-terminus for yeast expression Vectors pPIC9K-rhFNHN-29, pPIC9K-rhFNHC-36, and high-expression yeast expression strains were screened out, rhFNHN-29 polypep...

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Abstract

The invention discloses an application of recombinant FN heparin binding domain polypeptide on preparing medicaments resisting the invasion and the metastasis of malignant tumors. The recombinant FN heparin binding domain polypeptide comprises five I type homologous structures at the end N in FN molecules and contains 237 amino acids in Ser46-Gly 282. DNA sequences for encoding the polypeptide range from 403bp to 1113bp and have the length of 711bp, a PCR amplified fragment has the length of 741bp, and the length of the fragment after double enzyme digestion is 729bp. The molecular weight of the polypeptide expressed by yeast is 28.52kDa, and the polypeptide comprises three III type homologous structures in the FN molecules, i.e. III-12, III-13 and III-14 and contains 272 amino acids in Tyr1720-Tyr1991. DNA sequences for encoding the polypeptide range from 5428bp to 6244bp and have the length of 816bp, a PCR amplified fragment has the length of 835bp, and the length of the fragment after double enzyme digestion is 828bp. The molecular weight of the polypeptide expressed by the yeast is 36.09kDa. The experiments prove that the recombinant FN heparin binding domain polypeptide can be applied to preparing medicaments resisting the invasion and the metastasis of malignant tumors.

Description

Technical field: [0001] The invention relates to the field of medicine, in particular to the application of recombinant fibronectin N-terminal and C-terminal heparin-binding domain polypeptides in the preparation of drugs for resisting invasion and metastasis of malignant tumors. Background technique: [0002] Fibronectin, fibronectin, hereinafter referred to as FN; invasion and metastasis are the biological characteristics of malignant tumors. In the invasion and metastasis of tumor, the adhesion and dissociation between tumor cells and tumor cells, tumor cells and vascular endothelial cells and extracellular matrix play an important role in the metastasis and spread. In recent years, how the components of the extracellular matrix in tumors affect the adhesion and migration of tumor cells, thereby affecting the invasion and metastasis of tumors has become a research hotspot. FN is an important component of the extracellular matrix (ECM). It is a glycoprotein macromolecule ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61P35/00
Inventor 陈元仲黄涛邹起练陈显凌
Owner FUJIAN MEDICAL UNIV UNION HOSPITAL
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