Rapid detection kit of legionella bacteria and detection method thereof

A technology for detecting kits and strains, which is applied in the field of group bacteria detection, and can solve the problem of only detecting Legionella pneumophila, late, it generally takes a week or more to detect, non-pneumophila Legionella cannot be detected, etc. problems, achieving ease of use, good sensitivity and specificity

Active Publication Date: 2009-12-09
GUANGZHOU KINGMED DIAGNOSTICS CENT
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Problems solved by technology

One is to use the antigen-antibody reaction to detect Legionella specific antibodies or antigens. Such kits include Legionella ELISA kits and Legionella colloidal gold kits, because Legionella-related antigens or antibodies appear late in patients with Legionella infection. It usually takes a week or more to detect, and at the same time, it can only detect Legionella pneumophila, and has a certain cross-reaction, so this type of kit has certain limitations
The second category is to use molecu

Method used

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  • Rapid detection kit of legionella bacteria and detection method thereof
  • Rapid detection kit of legionella bacteria and detection method thereof
  • Rapid detection kit of legionella bacteria and detection method thereof

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Embodiment Construction

[0041] The present invention will be further described below in conjunction with accompanying drawing and specific embodiment:

[0042] The rapid detection kit for legionella species consists of bacterial genome DNA extraction solution, first PCR reaction solution, second PCR reaction solution, PCR product purification solution, enzyme digestion reaction solution, etc., and the above liquids are placed in containers respectively.

[0043] Wherein, the DNA extraction solution is used to extract bacterial genome DNA;

[0044] The first PCR reaction solution and the second PCR reaction solution are used to amplify Legionella specific gene fragments through two-step PCR reactions.

[0045] The PCR product purification solution is used together with the purification column to purify the PCR product.

[0046] The digestion reaction solution was used to digest the PCR products, and then the products were subjected to agarose gel electrophoresis.

[0047] The components and component ...

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Abstract

The invention discloses a rapid detection kit of legionella bacteria, comprising bacteria genomic DNA extracting solution, PCR reaction solution, PCR product purification solution and restriction endonuclease solution; wherein the solutions are respectively placed in a container; the PCR reaction solution comprises a first PCR reaction solution and a second PCR reaction solution; and the first PCR reaction solution and the second PCR reaction solution are respectively placed in containers. The invention also discloses a detection method of the kit. The invention can be used for detecting not only clinical samples such as the legionella bacteria in sputum and bronchial lavage fluid, but also the legionella bacteria in water sample, and has the advantages of high speed, simple and convenient and easy usage.

Description

Technical field: [0001] The invention relates to the technical field of legionella species detection, in particular to a Legionella species that can not only detect Legionella in clinical and environmental water samples, but also can be classified into Legionella pneumophila and non-pneumophila Legionella Rapid detection kit and detection method thereof. Background technique: [0002] At present, Legionella detection kits are mainly divided into two categories. One is to use the antigen-antibody reaction to detect Legionella specific antibodies or antigens. Such kits include Legionella ELISA kits and Legionella colloidal gold kits, because Legionella-related antigens or antibodies appear late in patients with Legionella infection. It usually takes a week or more to detect, and at the same time, it can only detect Legionella pneumophila, and has a certain cross-reactivity, so this type of kit has certain limitations. The second category is to use molecular biology methods t...

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Application Information

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IPC IPC(8): C12Q1/68C12R1/01
Inventor 朱庆义詹晓勇李连青胡朝晖张远志
Owner GUANGZHOU KINGMED DIAGNOSTICS CENT
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