Pollen specificity expression promoter and plant expression vector thereof

Abstract

The invention relates to a pollen specificity expression promoter and a plant expression vector thereof, wherein the promoter has a sequence under 1 item in a sequence list, with the length of 521bp, and a combined CaMV35S promoter replaces the plant expression vector pBI121 to construct a new plant expression vector which is named as pBITG6. The acquisition of the provided promoter creates conditions for a plant male sterile line and a restoring line obtained by using gene engineering, provides evidence for gene engineering research and provides basis for the specificity expression of an exogenous gene in the pollen. The exogenous gene can be a gene capable of coding disinsection toxin (targeting insects feeding on the pollen), a gene capable of increasing or decorating nutrient values of the pollen, and the like; and an excessive expression myrosase gene possibly has prevention function, and certain release function possibly on allergische rhinitis though applying prevention of pollen generation interfered by RNAi to roadside trees, thereby having important theoretical and practical meaning.

Description

technical field [0001] The present invention belongs to the fields of plant molecular biology and genetic engineering, and specifically relates to the acquisition of a novel myrosinase TGG6 gene promoter and the construction of a plant expression vector, especially to inducing the specific expression of foreign genes in pollen and obtaining plant males. The sterile line and restorer line have potential application value. Background technique [0002] Brassicaceae is a family with important economic value and theoretical research significance. They are widely distributed and contain a common secondary metabolite, glucosinolate, which is a relatively inert substance in biology. The component system, the mustard enzyme defense system, is commonly known as the "mustard bomb". Enzymes and substrates are stored in separate cells. When the plant is mechanically damaged, attacked by insects or infected by fungi and pathogens, the myrosinase located in the specific protein body is...

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Problems solved by technology

In addition, the overexpression of myrosinase gene may play a defensive role, and the application of RNAi interference to prevent pollen production in street trees may have a certain relief effect on pollen allergy, etc.

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