Bacillus subtilis oil suspending agent and preparation method thereof
A technology of Bacillus subtilis and oil suspension, which is applied in the field of oil suspension and its preparation, which can solve the problems of Bacillus subtilis loss, short storage time of water solution, increase of humidity in greenhouses, etc., and achieve high efficacy of pesticide application and increase of shelf life , the effect of increasing the survival time
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Embodiment 1
[0031] Embodiment 1: the preparation of bacillus subtilis oil suspension concentrate of the present invention
[0032] Weigh 2×10 11 200 grams of Bacillus subtilis parent powder of live spores / gram, 750 grams of methylated sunflower vegetable oil, 50 grams of OP-10 surfactants, mixed in a mixing kettle, and pulverized in a pump into a superfine pulverizing sand mill for 3 Hours, a stable oil suspension can be formed, the particle size is detected to be below 5 μm, and the concentration of Bacillus subtilis is 4×10 10 Viable spores / g.
Embodiment 2
[0033] Embodiment 2: the preparation of bacillus subtilis oil suspension concentrate of the present invention
[0034] Weigh 2×10 11 200 grams of Bacillus subtilis per gram of live spores, 600 grams of diluent (sunflower vegetable oil of methyl esterification), 200 grams of OP-10 surfactant, mix in a mixing kettle, and then pump into a superfine pulverizing sand mill Grinding in medium for 3 hours to form a stable oil suspension, the particle size is detected to be below 5 μm, and the concentration of Bacillus subtilis is 4×10 10 Viable spores / g.
Embodiment 3
[0035] Embodiment 3: the preparation of bacillus subtilis oil suspension concentrate of the present invention
[0036] Weigh 2×10 11 Live spores / gram Bacillus subtilis mother powder 10 grams, diluent (sunflower seed vegetable oil) 800 grams, SPAN-20 surfactant 190 grams, mix in the mixing kettle, then pump into the superfine pulverizing sand mill and pulverize 3 Hours, a stable oil suspension is formed, the particle size of the detected particles reaches below 5 μm, and the concentration of Bacillus subtilis is 2×10 9 Viable spores / g.
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