Polypeptide capable of combining with NKp80 receptor and application thereof

A technology of NK cells and drugs, applied in the field of immunology, can solve the problems of reduced curative effect, long production cycle, low permeability, etc., and achieve the effects of avoiding side effects, small molecular weight, and good solubility

Active Publication Date: 2010-02-03
IMMUNOPHARMACEUTIC INST OF HEFEI RUIDA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has a certain specificity and has a relatively good inhibitory effect on NK cells, but the preparation and purification of the recombinant AICL extracellular protein is costly and the production cycle is long; the protein has a large molecular weight, many amino acid residues, and is permeable in the body Low sensitivity, it is difficult to effectively reach the lesion site; macromolecular protein is easy to induce the body to produce antibodies, neutralize the drug protein, and reduce the curative effect

Method used

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  • Polypeptide capable of combining with NKp80 receptor and application thereof
  • Polypeptide capable of combining with NKp80 receptor and application thereof
  • Polypeptide capable of combining with NKp80 receptor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the synthesis of the polypeptide of the present invention and its functional detection

[0028] 1. Design and synthesis of peptide ligands for NKp80 receptor

[0029] 1. Design of peptide ligands for NKp80 receptors

[0030] 1) Establish the simulated three-dimensional structure of AICL

[0031] The three-dimensional structure of AICL has not yet been resolved. Searching in the protein database PDB found that among the proteins whose three-dimensional structure has been resolved, CD69 has the highest homology with AICL. Based on the three-dimensional structure of CD69 ( figure 1 A), according to the amino acid sequence of AICL provided by the Uniprot database (sequence number Q92478 in the database), the simulated three-dimensional structure of AICL was constructed through the calculation and simulation of the software 3D-JIGSAW ( figure 1 B)

[0032] 2) AICL conservative sequence screening

[0033] The amino acid sequence of AICL was analyzed by BLAS...

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Abstract

The invention discloses a polypeptide and the polypeptide provided in the invention is the polypeptide which is composed by amino acid sequence as shown in sequence one in the sequence table. The polypeptide of the invention can be combined with NKp80, can effectively seal off the binding sites between NKp80 and AICL, can inhibit the cytotoxic activity of NK cells, and can be used as an inhibitorof NK cells to treat autoimmune disease; in addition, the polypeptide has small molecular weight, seldom causes the immunological response of the organism; the polypeptide has good solubility and strong permeability, thus being easy to reach the site of action to exert the function.

Description

technical field [0001] The invention relates to the field of immunology, in particular to polypeptides capable of binding to NKp80 receptors and applications thereof. Background technique [0002] The most important type of cells in the natural immune system are natural killer cells (Natural Killer, NK cells), which kill infected cells and cancer cells in the body through granzymes, secretion of cytokines, TRAIL, FasL, etc. A series of activating receptors and inhibitory receptors are distributed on the surface of NK cells. After the inhibitory receptors bind to their ligands, they will transmit inhibitory signals to the cells and inhibit the activation of NK cells. The KIR receptor expressed on the surface of NK cells is a typical inhibitory receptor, which can recognize MHC-I molecules. Normal cells in the body express MHC-I molecules, which can transmit inhibitory signals through KIR receptors to prevent NK cells from being suppressed. Activation attacks normal cells. C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P37/02A61P43/00
Inventor 孙汭黄海华郑晓东田志刚
Owner IMMUNOPHARMACEUTIC INST OF HEFEI RUIDA CO LTD
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