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Method for curing and sterilizing culture media for large-scale cultivation of cordyceps militaris

A technology of Cordyceps militaris and sterilization method, which is applied in the field of maturation and sterilization of Cordyceps militaris culture medium, which can solve the problems of easy contamination of the culture medium, inability to handle large-capacity culture medium, etc., and achieve easy pollution, ripening and sterilization Improved work efficiency and high sensitivity

Active Publication Date: 2012-11-07
唐培宸
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Problems solved by technology

[0004] The purpose of the present invention is to propose a method for maturing and sterilizing the culture medium of Cordyceps militaris on a large scale, so as to solve the problem that the current maturing and sterilization methods cannot handle the culture medium in large capacity, and the culture medium is in the process of maturation and sterilization. It is easy to be infected with bacteria, causing a large number of mildew problems

Method used

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  • Method for curing and sterilizing culture media for large-scale cultivation of cordyceps militaris

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Embodiment 1

[0038] According to the description of the specific implementation method, 5 batches of culture boxes equipped with medium were subjected to the aging and sterilization of the present invention, with 20 culture boxes in each batch. After curing and sterilization, the material is 100% cured. After placing these culture boxes filled with culture medium for a culture cycle of Cordyceps militaris, no contamination of the culture medium was found.

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Abstract

The invention relates to a method for curing and sterilizing culture media, in particular to a method for curing and sterilizing culture media for large-scale cultivation of cordyceps militaris. The method for curing and sterilizing culture media for large-scale cultivation of cordyceps militaris is characterized in that the steps of curing and sterilizing are performed in combination; the temperature of curing and sterilization is between 100 and 135 DEG C; the time for curing and sterilization is between 40 and 50 minutes; curing and sterilization are synchronously performed in a double-door tunnel-type pressure steam sterilizer with a front door and a back door; the back door of the double-door tunnel-type pressure steam sterilizer is automatically locked when the front door is fed; and the front door of the double-door tunnel-type pressure steam sterilizer is automatically locked when materials are discharged from the back door. The method solves the problem that the prior curing-sterilizing technology is incapable of treating culture media in large capacity and easy to cause bacterial infection resulting in large-scale mould in the process of treating culture matrixes.

Description

technical field [0001] The invention relates to a method for maturing and sterilizing a medium, in particular to a method for maturing and sterilizing a culture medium of Cordyceps Militaris. Background technique [0002] Large-scale industrial production of Cordyceps militaris requires a large amount of culture medium. The medium for tissue culture of Cordyceps militaris is mainly composed of rice, soybean powder, various proteins and organic substances. The culture medium of tissue culture Cordyceps militaris is extremely prone to deterioration and moldy fermentation, so it must be matured and sterilized under high temperature and pressure before entering the inoculation process in a sterile state. The maturation and sterilization of the medium has become the key technology for large-scale factory cultivation of Cordyceps militaris. [0003] At present, the cultivation of Cordyceps militaris at home and abroad is generally a small-scale trial production, and there is no ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/07
Inventor 唐永范唐亮
Owner 唐培宸
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