Method for distant hybridization between subfamilies of red crucian carps and xenocypris davidi bleekers

Abstract

The invention discloses a method for the distant hybridization between subfamilies of red crucian carps and xenocypri davidi bleekers, comprising the following steps: firstly, selecting red crucian carps and xenocypri davidi bleekers with maturated gonads and good body features to be respectively used as female parent fishes and male parent fishes for hybridization, and carrying out artificial induced spawning on the female and male parent fishes in a breeding season; secondly, selecting the male and the female parent fishes with good effect of induced spawning for carrying out artificial dry-method insemination, and carrying out automatic hatching in running water after the insemination is finished; thirdly, breeding hatched fries, and detecting and screening the bred fries to obtain redcrucian carp and xenocypri davidi bleeker hybridized triploid fishes, red crucian carp and xenocypri davidi bleeker hybridized tetraploid fishes and natural gynogenesis red crucian carps. In the invention, the characteristics of the bred hybridized generation are improved, and the insemination rate and the hatching rate of distant hybridization are remarkably improved; in addition, the method is a new way for generating the tetraploid fishes and the natural gynogenesis red crucian carps, and has important biological significance in the aspects of the biological evolution and the genetic breeding of fishes.

Description

technical field [0001] The invention relates to a fish hybridization method, in particular to a distant hybridization method among subfamilies of carps. Background technique [0002] Hybridization technology is a commonly used method to improve fish traits, and hybridization between species or above is generally called distant hybridization. After distant hybridization, the hybrid offspring can integrate the excellent traits of the parents, making it possible to show the advantages of hybrid species in terms of fecundity, growth speed, disease resistance, body shape, and meat quality, thereby improving the traits of fish. For example, the hybrid offspring formed by the distant hybridization between red crucian carp and Xiangjiang wild carp has the advantages of fast growth, fresh and tender meat, strong disease resistance, and high survival rate; In addition to the above advantages of carp and carp hybrids, the hybrid offspring also have significantly higher body and back, ...

AI Technical Summary

Problems solved by technology

However, distant hybridization also has problems such as non-viability or low survival rate of hybrid offspring

[0003] No. CN1108469A Chinese patent document discloses a kind of hybridization and domestication method with red crucian carp as male parent and carp such as red carp as female parent. The hybridization and domestication method of the female parent, in the hybridization methods disclosed in these two documents, different species of fish in the same subfamily (Cyprinidae) are used as the parental fish, and the red crucian carp is used as the male parental fish for hybridization , the hybridization method is simple, and the degree of trait improvement in the offspring is not significant

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