Method for preparing l-selenomethionine by enzymatic resolution

Abstract

The invention discloses a method for preparing L-selenomethionine by using an enzyme resolution method, which comprises the following steps: 1. Crushing and separating fresh animal viscera with phosphate buffer solution; Dilute salt solution to make aminoacylase for later use; 2. Take NaOH and Na2CO3 and add water to make buffer solution, stir and add DL-selenomethionine to dissolve it, add acetic anhydride dropwise to adjust to negative, and use concentrated HCl to adjust the pH of the solution to 1-2 , sealed and placed to precipitate pale yellow crystals, filtered with suction, washed with deionized water, washed with ether until neutral, and dried to obtain the finished product of N-acetyl-DL-selenomethionine; 3. Use N-acetyl-DL-selenomethionine Dilute with deionized water, adjust with NaOH, add CoCl2 solution and aminoacylase, neutralize with acetic acid, decolorize with activated carbon, evaporate the filtrate to dryness, extract with ethyl acetate to obtain crude L-selenomethionine, and obtain L- Selenomethionine boutique.

Description

technical field [0001] The invention relates to a biological resolution method of amino acids, in particular to a method for preparing L-selenomethionine by using an enzyme resolution method. Background technique [0002] Selenium is one of the essential trace elements for the human body. It is a component of human red blood cell glutathione peroxidase (GSH-Px). Its main function is to participate in the synthesis of enzymes, protect the structure and function of the cell membrane, and prevent An important substance that is damaged and disturbed by excessive oxidation. Studies have shown that selenium deficiency can cause many human diseases, such as: anemia, coronary heart disease, Kashin-Beck disease, Keshan disease and diabetes. Supplementing selenium in an appropriate amount has the effects of enhancing the body's immune function and anti-cancer, preventing cancer, protecting the heart, and delaying aging. Scientific research has found that the normal intake of selenium...

AI Technical Summary

Problems solved by technology

Experiments have proved: 1. Inorganic selenium is more toxic to humans than organic selenium, and its bioavailability is far lower than organic selenium compounds

2. Excessive selenium has a certain degree of toxicity to the body, and the degree of toxicity is related to its chemical form

The amino acid bioseparation method is particularly effective with aminoacylase (E.C.3.5.1.14) as the enzyme source. This enzyme exists in various animals, plant organs, and microbial cells or fermentation broths, and is the organism that metabolizes amino acids. An important hydrolase in the process, with high activity, wide source, easy to obtain, and low production cost, it has been widely reported for large-scale amino acid resolution, but its application in the resolution of DL-selenomethionine is domestic and foreign. blank