A method for preparing silkworm eggs for transgenic eggs of dimorphic varieties of silkworms
A technology of transgenic and silkworm eggs, which is applied in the field of insect bioengineering, can solve the problems of long greening process, difficult breeding operation, increased transgenic operation, etc., to overcome the poor quality of cocoon silk, short greening incubation process, and overcome the amount of cocoon silk low effect
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Embodiment 1
[0034]Use spring-made diapause eggs of the silkworm dimorphic variety Dazao as the parent silkworm eggs, and use 22.5°C, 75%-85% relative humidity, and natural light conditions in the early stage to protect the 19th embryonic development stage; then use 20 ℃, relative humidity 75%-85%, and light intensity lower than 0.1lux under dark conditions to promote green protection until silkworm eggs hatch; after hatching, larvae are kept at 20°C-22°C, relative humidity 80%-90%, and natural light conditions. Use mulberry leaves to feed; silkworms from the third age start to use mulberry leaves to cocoon at 25°C-26°C, 75%-80% relative humidity, and natural light conditions; then at 24°C-26°C, 75%-80 %relative humidity, LD=18h: 6h under long-light conditions to protect until emergence; collect the female moths after emergence, and mate with male moths for 5 hours under weak light conditions below 24.5°C and 10lux. ;Mate the male and female moths separately, put the female moths on the ov...
Embodiment 2
[0036] The spring diapause silkworm eggs of the dimorphic silkworm variety Haoyue were used as the parent silkworm eggs, and the whole greening incubation process used dark conditions of 20°C, relative humidity of 75%-85%, and light intensity lower than 0.1lux; 20°C-21°C, relative humidity 85%-90%, and natural light conditions, use mulberry leaves to feed; 3rd instar silkworms start to use mulberry leaves in 25.5°C-26°C, 75%-80% relative humidity, natural light conditions Breed until cocooning; then protect until eclosion under the conditions of 25°C-26°C, 75%-80% relative humidity, LD=18h:6h long-light system; collect eclosion female moths, 25°C-26°C, below 10lux Mate with male moths for 4 hours under weak light conditions, and use ordinary Haoyue male moths for male moths; mating male and female moths separately, put female moths on oviposition paper for production, and lay eggs in a dark environment below 0.1lux at 25°C; collect eggs produced within 10 minutes Eggs are used...
Embodiment 3
[0038] Use the diapause eggs of the silkworm dimorphic variety 75 in spring as the parent silkworm eggs, and use 24.5°C-25°C, 75%-80% relative humidity, and natural light to protect them until the 19th stage of embryonic development; Then use 20°C, relative humidity of 80%-85%, and light intensity lower than 0.1lux to protect the dark conditions until hatching; after hatching, the larvae are kept at 21°C-22°C, relative humidity of 80%, and natural light conditions, using mulberry leaves Breeding; silkworms from the 3rd instar are raised at 25°C-26°C, 75%-80% relative humidity, and natural light conditions, using mulberry leaves to raise cocoons; then at 25°C-26°C, 75%-80% relative humidity , LD=18: 6h under long-light conditions to protect to eclosion; collect the female moths after eclosion, and mate with male moths under 25°C-26°C, 10lux weak light conditions for 5h, and the male moths use ordinary 75 new male moths; mating separately Male and female moths, female moths are ...
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