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Extreme halophilic bacterium, compound halophilic bacterium agent and application thereof

A technology of halophilic bacteria and bacterial agent, which is applied in the field of microorganisms to maintain the concentration of microorganisms, increase the volume load, and realize the effect of resource treatment.

Inactive Publication Date: 2012-04-18
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] So far, there is no efficient, economical and practical treatment method for high-salt organic wastewater.

Method used

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  • Extreme halophilic bacterium, compound halophilic bacterium agent and application thereof
  • Extreme halophilic bacterium, compound halophilic bacterium agent and application thereof
  • Extreme halophilic bacterium, compound halophilic bacterium agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Screening of extreme halophilic bacteria

[0051] 1. Isolation of extreme halophilic bacteria strains

[0052] 1.1) The in-situ multi-point method collects sludge samples from sludge pools and sewage outlets of salt chemical enterprises;

[0053] 1.2) Carry out enrichment culture at 25-35° C. for 3-10 days in a liquid enrichment medium containing 16wt%-26wt% of salt in the collected sludge-like sludge;

[0054] 1.3) Separating and cultivating the enriched and cultivated sludge-like sludge in the separation medium at 25-35°C to obtain isolated single strains; then carrying out 2- 6 times of purification and isolation culture, so as to select 23 strains of extreme halophilic bacteria with a salinity tolerance of 15-25%; the purification and isolation medium used has the same components and the content of each component as the above-mentioned isolation medium;

[0055] The components of the liquid enrichment medium and the contents of each component are as foll...

Embodiment 2

[0068] Example 2 Preparation of compound halophilic bacteria agent

[0069] 1) Cultivate the above three strains of extreme halophilic bacteria in a compound culture medium at 25-35°C for 7 days to 10 days, wherein the strain CGMCC No.3081: CGMCC No.3082: CGMCC No.3083 is 3: 3:1, to obtain compound extreme halophilic bacteria agent;

[0070] The components of the compound microbial agent culture medium and the contents of each component are: yeast extract 10g / L, peptone 15g / L, MgSO 4 ·7H 2 O 20g / L, KCl 2g / L, NaCl 180g / L, pH 7.0.

[0071] 2) Expanding the above-mentioned compound extreme halophilic bacteria agent

[0072] Put the above compound extreme halophilic bacteria agent in the expansion medium of compound halophilic bacteria agent at 25-30°C for expanded culture for 7-10 days to obtain the compound halophilic bacteria agent;

[0073] The components of the compound bacterial agent expansion medium and the contents of each component are: yeast extract 10g / L, peptone 1...

Embodiment 3

[0074] Example 3 Simulation experiment of using three strains of extreme halophilic bacteria of the present invention to treat the cyanuric chloride production wastewater of "Degussa Sanzheng (Yingkou) Fine Chemical Co., Ltd."

[0075] Use the combination of three extreme halophilic bacteria of the present invention to process the high-salt cyanuric chloride production wastewater containing 18wt% of salt in "Degussa Sanzheng (Yingkou) Fine Chemical Industry Co., Ltd.":

[0076] 1. First prepare the compound test bacterial agent:

[0077] Cultivate the above-mentioned three strains of extreme halophilic bacteria of the present invention in a compound culture medium at 25°C for 10 days to obtain a compound halophilic bacteria agent;

[0078] The components of the compound bacterial culture medium are: yeast extract 10g / L, peptone 15g / L, MgSO 4 ·7H 2 O 20g / L, KCl 2g / L, NaCl 180g / L, pH 7.0;

[0079] The above-mentioned composite halophilic bacteria agent is carried out to expan...

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Abstract

The invention relates to the field of microorganisms, in particular to an extreme halophilic bacterium, a compound halophilic bacterium agent and application thereof. The extreme halophilic bacteria obtained through screening are respectively salt monad CGMCC NO.3081, pseudomonad CGMCC NO.3082 and bacillus CGMCC NO.3083. The invention also provides the compound halophilic bacterium agent containing the extreme halophilic bacterium. The invention obtains three extreme halophilic bacterium strains with the salt tolerance of 15-25 percent through separation, a compound extreme halophilic bacterium agent formed by compounding the three extreme halophilic bacterium strains can effectively process high salt cyanuric chloride production waste water, and compared with other processing technologies, the method better conforms to the state policies of circular economy and energy saving and emission reduction, can save a large number of running cost and realize the resource processing of waste water.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to extreme halophilic bacteria, compound halophilic bacteria bacterial agents and applications. Background technique [0002] With the acceleration of my country's industrialization process, high-salt wastewater will be produced in many production areas, such as printing and dyeing, papermaking, chemical industry, pesticides, oil extraction, seafood processing, etc. This type of wastewater usually contains high concentrations of organic pollutants, and direct discharge will be harmful to the environment. The environment is seriously polluted. Moreover, high-salt organic wastewater is not suitable for conventional biological treatment due to the poisonous effect of salt on common microorganisms. This is mainly because: (1) when the salt concentration is too high, the osmotic pressure of the water body is high, which will easily dehydrate the microbial cells and cause the separation of c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/00C02F3/34C02F3/30C12R1/01C12R1/38C12R1/07
CPCC12N1/20C12R1/38C02F3/34C12R1/01C12P39/00C12R1/07C12N1/205C12R2001/01C12R2001/07C12R2001/38
Inventor 李捍东
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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