Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting liensinine, isoliensinine and neferine from lotus plumule

A kind of technology of isiensinine and isiensinine, is applied in the field of isoliensinine, and isiensinine, extraction and separation of high-purity isiensinine, can solve the problems that cannot meet market demand, product purity is low, production cost is high, achieves shortening. Separation time, high purity, and the effect of saving reagents

Inactive Publication Date: 2011-09-07
LIAOCHENG UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above-mentioned various methods may obtain products with lower purity, or lower yields, or use more complicated systems, and some have higher production costs and smaller production scales, which cannot meet market demand.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting liensinine, isoliensinine and neferine from lotus plumule
  • Method for extracting liensinine, isoliensinine and neferine from lotus plumule
  • Method for extracting liensinine, isoliensinine and neferine from lotus plumule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 100g of crushed lotus seed heart medicinal material, add 0.3L 50% ethanol, heat and reflux for 60 minutes, separate the residue and extract; add 0.3L 50% ethanol to the residue and reflux twice, each time for 40 minutes, separate the residue and extract; combine the extracts obtained by reflux for the above 3 times, and recycle ethanol by distillation under reduced pressure to obtain the crude extract of lotus seed heart.

[0027]Suspend the above crude extract in water, put it on a D101 macroporous adsorption resin column, elute with 10% ethanol of 10 times the column volume, and discard the eluent. Then elute with 40% ethanol until there is no liensinine, isoliensinine and methyl-liensinine in the effluent, collect the eluate, and distill under reduced pressure to recover the solvent to obtain the extract of lotus root.

[0028] Diethyl ether-phosphate buffer was used as a high-speed countercurrent chromatography two-phase solvent system to separate and purify th...

Embodiment 2

[0030] Take 100g of crushed lotus seed heart, add 2L of 55% ethanol, heat and reflux for 20 minutes, separate the residue and extract; add 1L of 55% ethanol to the residue and reflux twice, each time for 10 minutes, separate the residue and extract liquid; combine the extracts obtained by reflux for the above 3 times, and recycle ethanol by distillation under reduced pressure to obtain the crude extract of lotus seed heart.

[0031] Suspend the above-mentioned crude extract extract in water, put it on HPD300 macroporous adsorption resin column, elute with 30% ethanol of 5 times the column volume, and discard the eluent. Then elute with 80% ethanol until there is no liensinine, isoliensinine and methyl-liensinine in the effluent, collect the eluate, and distill under reduced pressure to recover the solvent to obtain the extract of lotus root.

[0032] Diethyl ether-phosphate buffer was used as a high-speed countercurrent chromatography two-phase solvent system to separate and p...

Embodiment 3

[0034] Take 100g of crushed lotus seed heart, add 0.5L 60% ethanol, heat and reflux for 45 minutes, separate the residue and extract; add 0.5L 60% ethanol to the residue and reflux twice, each time for 30 minutes, separate the residue and extract; combine the extracts obtained by reflux for the above 3 times, and recycle ethanol by distillation under reduced pressure to obtain the crude extract of lotus seed heart.

[0035] Suspend the above-mentioned crude extract extract in water, put it on a D101 macroporous adsorption resin column, and elute with 15% ethanol of 10 times the column volume, and discard the eluent. Then elute with 60% ethanol until there is no liensinine, isoliensinine and methyl-liensinine in the effluent, collect the eluate, and distill under reduced pressure to recover the solvent to obtain the extract of lotus root.

[0036] Diethyl ether-phosphate buffer was used as a high-speed countercurrent chromatography two-phase solvent system to separate and purif...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting liensinine, isoliensinine and neferine from lotus plumule, which adopts the lotus plumule as a raw material and comprises the following steps of: (1) preparing a crude extract; (2) preseparating by adopting macroporous resin; (3) purifying by adopting a high speed counter current chromatography to obtain the liensinine, the isoliensinine and the neferine with the purity of more than 98 percent. By extracting alkaloid in a lotus plumule medicinal material by adopting ethanol, carrying out preseparation by adopting macroporous resin, adopting theethanol as an eluant and carrying out purification by adopting the high speed counter current chromatography, the process is environmental-friendly without serious damage to the environment.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for extracting and separating high-purity liensinine, isoliensinine and methyl-liensinine from the heart of lotus seeds, a Chinese herbal medicine. Background technique [0002] Lotus seed heart (Nelumbo nucifera) is the green young leaves and radicles of lotus seeds of Nymphaeaceae plants. It is bitter and cold in nature and has the effect of clearing heart and removing heat. The main active ingredient is alkaloids. Liensinine has a good antiarrhythmic effect, which can significantly resist the arrhythmia induced by aconitine in rats and guinea pigs induced by ouabain; it can also prevent the occurrence of ventricular fibrillation in guinea pigs caused by adrenaline; it can also resist myocardial infarction. Cardiac arrhythmia in rats induced by blood reperfusion. Isoliensinine intravenous injection can dose-dependently and transiently reduce the heart rate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D217/20
CPCY02P20/582
Inventor 孙爱玲柳仁民
Owner LIAOCHENG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com