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Method for extracting high pure chicoric acid from Coneflower

A technology of cichoric acid and echinacea, which is used in pharmaceutical formulations, separation/purification of carboxylic acid esters, medical preparations containing active ingredients, etc. High, simple and fast effect

Inactive Publication Date: 2011-07-20
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Large-scale industrial production is not yet possible

Method used

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  • Method for extracting high pure chicoric acid from Coneflower
  • Method for extracting high pure chicoric acid from Coneflower

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 1. Ethanol extraction: Take 100g of the ground dried echinacea root, add 3-15 times the amount of 95% ethanol solvent, soak at room temperature for 24 hours, heat to reflux for 3 hours, and separate the medicinal solution. Add 95% ethanol of 10 times the amount of the drug residue, heat to reflux for 2 hours, and separate the drug solution. Combine the extracted medicinal solution, and use methods such as filtration and centrifugation to remove impurities in the extract. The extraction solvent is recovered by distillation or vacuum distillation to obtain the ethanol extract of Echinacea purpurea.

[0024] Macroporous adsorption resin column pre-separation: Put the ethanol extract obtained above on an AB-8 macroporous adsorption resin column, first rinse with water until the effluent is colorless, and then eluted with 10 times the column volume of 5% ethanol, and collect The 70% ethanol eluate was distilled under reduced pressure to recover the solvent to obtain cichoric a...

Embodiment 2

[0027] Take 100g of the crushed Echinacea root, add 1.5L of 40% ethanol, soak at room temperature for 4 hours, heat to reflux for 3 hours, and separate the medicinal solution. Add 1L of 95% ethanol, heat to reflux for 2 hours, and separate the liquid medicine. The extracted liquids are combined, and the impurities in the extracted liquid are removed by filtration. The extraction solvent is recovered by vacuum distillation to obtain the ethanol extract of Echinacea purpurea.

[0028] The ethanol extract obtained above was applied to the AB-8 macroporous adsorption resin column, washed with water until the effluent was colorless, and then eluted with 10 times the column volume of 30% ethanol, collected the 30% ethanol eluate, and reduced The solvent was recovered by pressure distillation to obtain cichoric acid extract.

[0029] Use petroleum ether-ethyl acetate-methanol-acetic acid water as the high-speed countercurrent chromatography two-phase solvent system to separate and purif...

Embodiment 3

[0035] Take 100g of the crushed Echinacea root, add 1.5L of 40% ethanol, soak at room temperature for 4 hours, heat to reflux for 3 hours, and separate the medicinal solution. Add 1L of 95% ethanol, heat to reflux for 2 hours, and separate the liquid medicine. The extracted liquids are combined, and the impurities in the extracted liquid are removed by filtration. The extraction solvent is recovered by vacuum distillation to obtain the ethanol extract of Echinacea purpurea.

[0036] The ethanol extract obtained above was applied to the AB-8 macroporous adsorption resin column, washed with water until the effluent was colorless, and then eluted with 10 times the column volume of 30% ethanol, collected the 30% ethanol eluate, and reduced The solvent was recovered by pressure distillation to obtain cichoric acid extract. The purity of the prepared cichoric acid can reach more than 99%, and the yield can reach more than 90%.

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Abstract

The present invention relates to process of extracting high purity chicoric acid from Echinacea purpurea. The process includes the following steps: 1. extracting crushed Echinacea purpurea root with alcohol and separating to eliminate solid impurity; 2. pre-separating the extracted liquid after recovering alcohol in a macroporous resin column through eluting with alcohol solution of different concentrations to obtain the extract; and 3. purifying the extract through one high speed counter current chromatographic process to obtain chicoric acid of purity over 99 %.

Description

Technical field [0001] The invention relates to a traditional Chinese medicine preparation and a preparation method thereof, in particular to a method for preparing high-purity cichoric acid by using echinacea as a raw material. Background technique [0002] Echinacea is an immune booster and immunomodulator that has received widespread attention in the world. Its main physiologically active ingredient is cichoric acid. Modern pharmacological studies have shown that cichoric acid can enhance immune function, anti-inflammatory, inhibit hyaluronidase, protect collagen Ⅲ from free radicals that can cause degradation, and inhibit HIV-1 and HIV-1 integrase. . [0003] The method for extracting and separating cichoric acid from Echinacea purpurea has been reported in the literature, such as Wu Qilin et al. [Research on the preparation of cichoric acid using macroporous adsorption resin adsorption and separation technology; Journal of Beijing University of Chemical Technology, 2004 05] i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C67/48C07C69/732A61K36/287A61P37/02
Inventor 孙爱玲柳仁民
Owner LIAOCHENG UNIV
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