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Preparation method for Lepidium meyenii alkaloid

A technology for maca alkaloids and crude extracts, applied in the direction of organic chemistry and the like, can solve the problems of large amount of solvent consumption, low recovery rate of alkaloids, complicated procedures, etc., and achieves reduction of production costs, short process cycle, and high extraction efficiency. Effect

Inactive Publication Date: 2016-07-06
HEILONGJIANG JOHNSUN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]The total alkaloid content in maca is very low, only containing 0.2%-0.4%, which brings difficulties to the extraction and separation and purification of maca alkaloids. At present, the extraction and purification process of maca alkaloids in the existing literature and patents is complex and requires multi-step column chromatography purification. This method is not only complicated in process, time-consuming and laborious, but also has a low recovery rate of alkaloids and consumes too much solvent , the cost is too high, not suitable for large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Take 2kg of maca powder, add 12L of 20% ethanol solution, mix well, ultrasonically extract for 40 minutes, filter, and ultrasonically extract the residue twice according to the above method, combine the filtrate and recover under reduced pressure until it has no alcohol smell, to obtain an extract. Dissolve the above extract with 1% HCI, filter, adjust the pH value of the filtrate to 9 with sodium hydroxide, then extract with chloroform, concentrate the extract under reduced pressure, and dry to obtain a crude extract of maca alkaloids.

[0017] (2) Mix n-hexane, ethyl acetate, methanol, and water in a volume ratio of 5:1:5:1, let stand overnight in a separatory funnel, separate the upper and lower phases, and degas the upper and lower phases by ultrasonic degassing for 20 minutes for later use. Take 200mg of maca alkaloid crude extract and dissolve it in 20ml of the lower phase, shake it to dissolve completely, and prepare for high-speed countercurrent chromatog...

Embodiment 2

[0021] (1) Take 5kg of maca powder, add 50L of 60% methanol solution, mix well, extract by microwave for 25 minutes, filter, extract the residue once more according to the above method, combine the filtrate and recover it under reduced pressure until it has no alcohol smell, and obtain the extract; use 2.5 % acetic acid to dissolve the above-mentioned extract, filter, adjust the pH value of the filtrate to 10 with ammonia water, then extract with ethyl acetate, concentrate the extract under reduced pressure, and dry to obtain the crude extract of maca alkaloids.

[0022] (2) Mix n-hexane, ethyl acetate, methanol, and water in a ratio of 5:5:7:5 by volume, let stand overnight in a separatory funnel, separate the upper and lower phases, and add 10 mM triethylamine to the upper phase 10mM HCI was added to the lower phase, and ultrasonically degassed for 15min for later use. Take 300mg of maca alkaloid crude extract and dissolve it in 20ml of the lower phase, shake it to disso...

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Abstract

The invention relates to a preparation method for Lepidium meyenii alkaloid. The preparation method comprises the specific steps: (1) carrying out extracting; (2) carrying out impurity removal; (3) carrying out high-speed countercurrent purification; and (4) carrying out column-chromatography purification. The preparation method for rapidly and efficiently separating a Lepidium meyenii alkaloid monomer, disclosed by the invention, is a method for separating and preparing the Lepidium meyenii alkaloid monomer from Lepidium meyenii by using high-speed countercurrent chromatography and normal-phase chromatography. According to the preparation method, a crude Lepidium meyenii alkaloid extract is firstly subjected to impurity removal through chloroform extracting, then, is subjected to purification by the high-speed countercurrent chromatography and finally is further purified by using the normal-phase chromatography so as to obtain the Lepidium meyenii alkaloid monomer; and the process flow is green and environment-friendly and is free of severe hazards to environments, the operation is simple, the process cycle is short, reagents are saved, an eluant can be recycled, the production cost is reduced, and the extracting efficiency is high, so that the preparation method is applicable to industrial production.

Description

technical field [0001] The invention relates to the field of separation and purification of natural products, in particular to a preparation method of maca alkaloids. Background technique [0002] Maca (LepidiummeyeniiWalp.) is a herbaceous plant of the Brassicaceae genus Lepidium. It is native to the Andes Mountains at an altitude of 500m-4450m in Peru. It has the reputation of "Peruvian ginseng". High nutritional value, reasonable nutritional structure and a variety of active secondary metabolites make maca have a variety of nutritional health functions and pharmacological effects, which can be used to enhance energy, improve fertility, improve sexual function, treat menopausal syndrome, rheumatism, Depression, anemia, etc., as well as anti-cancer and anti-leukemia effects. [0003] Studies have shown that alkaloids are one of the main active ingredients of Maca. Maca alkaloids act on the pituitary gland and hypothalamus, regulate endocrine glands (such as adrenal gland,...

Claims

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Application Information

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IPC IPC(8): C07D233/58C07D233/60
Inventor 陈喜军
Owner HEILONGJIANG JOHNSUN BIOLOGICAL ENG CO LTD
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