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Method for separating and purifying monomer compounds from Chinese medicinal herb rhizoma coptidis

A technology for separation and purification of compounds, applied in the field of separation and purification of monomeric compounds, which can solve problems such as failure to meet market demands, complex solvent systems, and high production costs, and achieve the effects of shortened separation time, good separation effect, and low cost

Inactive Publication Date: 2012-09-12
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Above-mentioned various methods or can only obtain single component product, or the product purity that obtains is lower, or use the solvent system that is harmful to environment and human body, or solvent system is more complicated, and the production cost that has is higher, and production scale is smaller, cannot to satisfy the market's needs

Method used

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  • Method for separating and purifying monomer compounds from Chinese medicinal herb rhizoma coptidis
  • Method for separating and purifying monomer compounds from Chinese medicinal herb rhizoma coptidis
  • Method for separating and purifying monomer compounds from Chinese medicinal herb rhizoma coptidis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 500g of crushed Coptis chinensis, add 8L of 40% ethanol, heat and reflux for 120 minutes, separate the residue and extract; add 6L of 40% ethanol to the residue and reflux twice, each time for 90 minutes, separate the residue and extract ; Combine the extracts obtained by refluxing the above three times, and recycle ethanol by distillation under reduced pressure to obtain the crude extract of Coptidis Rhizome.

[0030] Dissolve the crude extract of Coptis chinensis with 0.2L of 8.0% hydrochloric acid, let it stand for 36 hours, and then filter to remove the precipitate to obtain a solution; adjust the pH of the above solution to 8 with ammonia water, and add sodium carbonate to it until the concentration of sodium carbonate is 4%. , After standing for 24 hours, the precipitate was obtained by filtration, and the precipitate was dried to obtain the crude extract of coptis alkaloids.

[0031] N-butanol-phosphate buffer was used as high-speed countercurrent chromatogr...

Embodiment 2

[0033] Take 500g of crushed Coptis chinensis, add 6L of 55% ethanol, heat and reflux for 90 minutes, separate the residue and extract; add 6L of 55% ethanol to the residue and reflux twice, each time for 50 minutes, separate the residue and extract ; Merge the extracts obtained by reflux for the above 3 times, and reclaim ethanol by distillation under reduced pressure to obtain the crude extract of coptisine.

[0034] Dissolve the crude extract of Coptidis rhizome with 0.3L of 5.0% hydrochloric acid, let it stand for 36 hours and filter to remove the precipitate to obtain a solution; adjust the pH of the above solution to 10 with ammonia water, add sodium carbonate to it until the concentration of sodium carbonate is 0.5% , After standing for 36 hours, the precipitate was obtained by filtration, and the precipitate was dried to obtain the crude extract of coptis alkaloids.

[0035] Using n-butanol-phosphate buffer as the high-speed countercurrent chromatography two-phase solve...

Embodiment 3

[0037] Take 500g of crushed Coptis chinensis, add 3L of 80% ethanol, heat and reflux for 80 minutes, separate the residue and extract; add 3L of 80% ethanol to the residue and reflux twice, each time for 50 minutes, separate the residue and extract ; Merge the extracts obtained by reflux for the above 3 times, and reclaim ethanol by distillation under reduced pressure to obtain the crude extract of coptisine.

[0038] Dissolve the crude extract of Coptis chinensis with 2.0L of 0.4% hydrochloric acid, let it stand for 12 hours, and then filter to remove the precipitate to obtain a solution; adjust the pH of the above solution to 8 with ammonia water, and add sodium carbonate to it until the concentration of sodium carbonate is 0.4% , After standing for 24 hours, the precipitate was obtained by filtration, and the precipitate was dried to obtain the crude extract of coptis alkaloids.

[0039] N-butanol-phosphate buffer was used as high-speed countercurrent chromatography two-pha...

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Abstract

The invention relates to a method for extracting epiberberine, coptisine, palmatine and berberine from golden thread. In the method, the golden thread is used as a raw material. The method comprises the following steps: (1) preparing crude extractive; (2) pre-separating by acid dissolution and alkaline precipitation; and (3) purifying with high-speed countercurrent chromatograph so as to obtain the epiberberine, the coptisine, the palmatine and the berberine, the concentrations of which are above 98%. In the method, ethanol is used for extracting alkaloid in a golden thread medicinal material, pre-separation is carried out by acid dissolution and alkaline precipitation, and purification is carried out with high-speed countercurrent chromatograph, thus the method disclosed by the inventionhas an environment-friendly process and no serious damage on environment.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for separating and purifying monomeric compounds (epiberberine, coptisine, palmatine and berberine) from Chinese medicine Coptis chinensis. Background technique [0002] Coptis chinensis is the dried rhizome of Coptis chinensis, Coptis triangularis or Yunlian, which are commonly known as "Weilian", "Yalian" and "Yunlian" respectively. They are well-known and commonly used traditional Chinese medicines and are frequently used in prescriptions. . Coptis chinensis bitter, cold, heart-guiding, spleen, stomach, liver, gallbladder, large intestine meridian, has the effects of clearing away heat and dampness, purging fire and detoxifying. The main components of Coptidis Rhizoma are alkaloids, which mainly contain berberine, palmatine, coptisine, epiberberine and jatrorrhizine and other berberine alkaloids. [0003] There are existing literature reports on the meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D455/03
CPCY02P20/582
Inventor 柳仁民汪海兵孙爱玲
Owner LIAOCHENG UNIV
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