Method for preparing Chinse clinopodium herb total saponins

A technology for cutting blood flow and total saponins, which is applied in the field of preparing blood flow total saponins by precipitation method, which can solve problems such as complicated operations, achieve high extraction efficiency, low energy consumption, and facilitate recovery and reuse

Inactive Publication Date: 2010-08-18
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] And the patent 200510038206.4 "preparation method of total saponins of traditional Chinese medicine blood flow", the total saponins of the product obtained by this method is greater than 50%, and the Buddleia saponins are greater than 5%. The disclosed technology is water extraction, alcohol precipitation, resin purification, silica gel magnesium oxide Multiple processes such as chromatography and n-butanol extraction, the operation is complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 5kg of blood-cutting herbs, crush them into an ultrasonic extraction tank, add 35L of 50% methanol solution, and conduct ultrasonic extraction at a frequency of 40KHz for 2 hours, extract twice or twice, combine the extracts and filter, concentrate under reduced pressure to a density of 1.2, and release 400ml of liquid into Add 1200ml of n-butanol into the extraction tank for full extraction, extract 3 times, combine the n-butanol extract, add 100ml of acetone after recovering n-butanol, stir evenly and place it for precipitation, filter out the precipitate after sufficient precipitation, and recover the acetone for reuse . After refluxing and dissolving the precipitate with 900ml of n-butanol, add 9g of activated carbon for injection and 27g of alumina for decolorization, filter, add 90ml of acetone to the filtrate, stir fully, static precipitation, dissolve, decolorize and precipitate 3 times, vacuum dry the precipitate, Obtain product 150g total saponin content ...

Embodiment 2

[0022] Take 5kg of blood-cutting medicinal materials, crush them into an ultrasonic extraction tank, add 35L of 70% methanol solution, and conduct ultrasonic extraction at a frequency of 80KHz for 1 hour, extract twice or twice, combine the extracts and filter, concentrate under reduced pressure to a density of 1.1, and release 370ml of liquid into Add 1500ml of n-butanol into the extraction tank for full extraction, extract twice, combine the n-butanol extract, add 120ml of acetone after recovering n-butanol, stir evenly and place it for precipitation, filter out the precipitate after sufficient precipitation, and recover the acetone for reuse . After refluxing and dissolving the precipitate with 1000ml of n-butanol, add 20g of activated carbon for injection and 50g of alumina for decolorization, filter, add 200ml of acetone to the filtrate, stir thoroughly, statically settle, dissolve, decolorize and settle twice, and vacuum dry the precipitate. Obtain product 130g total sap...

Embodiment 3

[0024] Take 5kg of blood-cutting herbs, crush them into an ultrasonic extraction tank, add 25L of 60% ethanol solution, and conduct ultrasonic extraction at a frequency of 60KHz for 1.5 hours, extract two or three times, combine the extracts and filter, concentrate under reduced pressure to a density of 1.12, and release 380ml of liquid into Add 1200ml of n-butanol into the extraction tank for full extraction, extract 3 times, combine the n-butanol extract, add 150ml of acetone after recovering n-butanol, stir evenly and place it for precipitation, filter out the precipitate after sufficient precipitation, and recover the acetone for reuse . After refluxing and dissolving the precipitate with 1000ml of n-butanol, add 7g of activated carbon for injection and 28g of alumina for decolorization, filter, add 100ml of acetone to the filtrate, stir fully, static precipitation, dissolve, decolorize and precipitate 3 times, vacuum dry the precipitate, Obtain product 145g total saponin ...

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Abstract

The invention relates to a method for preparing Chinse clinopodium herb total saponins. The technology steps are as follows: carrying out ultrasonic extraction on methanol or ethanol solution, extracting for 2-3 times with normal butanol, recovering normal butanol, adding acetone for precipitation, normal butanol refluxing and dissolving, adding activated carbon and aluminum oxide to remove impurity, adding acetone and precipitating for 2-3 times and the like. The Chinse clinopodium herb total saponins prepared by the method of the invention have low energy consumption, less waste liquor, short period and high product content; and the obtained product can be directly used as raw materials of medicine and health care products.

Description

Technical field: [0001] The invention belongs to the technical field of bioseparation, in particular to a method for preparing total saponins from blood flow through a precipitation method. Background technique: [0002] The traditional Chinese medicine Duanxueliu is the dry above-ground part of the Labiatae plant Pinnata and Yinfenglun. It was originally a herbal medicine excavated from Anhui Province for folk use. Contains compounds such as saponins, flavonoids, amino acids, polysaccharides, phenolic substances, and volatile oils. It is mainly used to treat bleeding disorders such as metrorrhagia, uterine fibroids, hematuria, epistaxis, bleeding gums, and wound bleeding. Duoxueliu is one of the few single-herb medicines selected from folk prescriptions in the 1970s and entered into the National Pharmacopoeia after clinical verification. It has definite curative effect and high safety, and is recognized as the first choice of oral gynecological hemostatic medicine in Chines...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P7/04
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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