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Gene chip for detecting CryIAc-gene-containing transgenic plant and product and application thereof

A technology of transgenic plants and gene chips, which is applied in the field of biochips, can solve problems such as the patent report of gene chip detection technology that has not yet discovered the CryIAc gene, and achieve the effects of good specificity, improved supervision level, and high sensitivity

Inactive Publication Date: 2013-01-02
山东鲁保科技开发有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After searching the existing patents and other literatures, no patent report on gene chip detection technology of CryIAc gene has been found

Method used

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  • Gene chip for detecting CryIAc-gene-containing transgenic plant and product and application thereof
  • Gene chip for detecting CryIAc-gene-containing transgenic plant and product and application thereof
  • Gene chip for detecting CryIAc-gene-containing transgenic plant and product and application thereof

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Experimental program
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Effect test

Embodiment 1

[0057] 1. Design of primers and probes

[0058] The primers were fluorescently labeled with Cy3-Amidite at the 5' end. The primers were synthesized by Shanghai Boya Biological Company, and the primers were diluted to 10 μmol / L for use.

[0059] Use Primer premier 5.0 and Oligo 6.0 software to design 40mer Oligo candidate probes based on the sequence of CryIAc. The general principles of design are: (1) have relatively similar TM values, and the fluctuation range is 5°C; (2) GC content is 45% ~55%, otherwise it will increase the background of non-specific hybridization; (3) The repeated single bases are not more than 4 in a row; (4) The length of the paired bases of the most stable secondary structure of the probe molecule is not greater than 4bp. The probe was modified with aminohexane at the 5' end, and the amino group and the probe sequence were connected with a spacer poly(dT15), so that the final probe length was 55 bp. All probes were synthesized in Shanghai Boya Biologi...

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Abstract

The invention discloses a gene chip for detecting CryIAc-gene-containing transgenic plant and product, which comprises a slide glass modified by surface aldehyde group, a probe coated with array, as well as positive control (HEX), hybridization positive control (PC), a positive quality control probe (18 Sr RNA), a negative quality control probe and blank control which are fixed by nucleic acid, wherein the probe of CryIAc gene has the sequence of NH2-(CH2) 6-O-Poly (dT) 15-TCAGAGAGTGGGGAAGCCGATC CTACTAACC CAG CTCTCCG. The gene chip for detecting the CryIAc-gene-containing transgenic plant and product has good specificity and high sensitivity, provides a simple and reliable detection method for the detection and analysis of the specificity of the CryIAc-gene-containing transgenic plant and product, and can be used for detection of the transgenic plant and the products thereof.

Description

technical field [0001] The invention relates to a gene chip for detecting transgenic plants and products containing gene CryIAc and its application, belonging to the technical field of biochips. Background technique [0002] In recent years, genetically modified crops such as corn, soybean, cotton, rapeseed and tomato have been approved for planting and production in many countries, and some have been processed into food, feed or used as food additives. Since the ecological safety and food safety of genetically modified products have been controversial, more than 40 countries and regions have successively implemented genetically modified product labeling systems. [0003] The establishment of genetically modified labeling systems in various countries has put forward strict requirements on the sensitivity and accuracy of genetically modified detection technology, and various genetically modified detection technologies have also become research hotspots. Commonly used transge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 路兴波武海斌孙红炜李凡
Owner 山东鲁保科技开发有限公司
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