A method for displaying and labeling the scene under the microscope according to the accurate magnification on the screen
A microscope and accurate technology, applied in microscopes, optics, instruments, etc., can solve problems such as labeling, inaccuracy, and irresponsible misleading by observers
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Embodiment 1
[0053] Example 1. Ideally restore the color and intensity of the scene under the microscope:
[0054] The first step is to turn the potentiometer control knob of the microscope light source to a large enough size according to the conventional microscopic imaging requirements. After a clear focused scene with suitable brightness is observed in the eyepiece, move the slide on the stage, Make the objective lens and the condenser directly opposite, start the computer imaging software to detect the dynamic brightness of the image displayed on the screen, adjust the light quantity adjustment button of the condenser, and fix the dynamic brightness of the image displayed on the screen at 230 gray values;
[0055] The second step is to turn on the automatic white balance and automatic exposure compensation functions of the image sensor driver software or imaging software. After the blank image displayed on the screen automatically turns gray, turn off the automatic white balance and automati...
Embodiment 2
[0058] Embodiment 2. The integer magnification of the image displayed on the screen is indicated:
[0059] The first step is to calibrate the imaging system: first place the microscope objective micrometer (a special glass slide whose center is divided into 100 equal parts within a distance of 1mm by the scale line) under the observation objective of the microscope, and pass through the eyepiece Observe the image and adjust the focus clearly. At the same time, open the image sensor and imaging system software to display the image on the screen and adjust the focus until the image is clear; turn the image sensor until the scale line of the micrometer is completely perpendicular to the screen, find two at random Enter the scale line on the screen that is far away in the horizontal direction and input its scale information into the imaging system software; turn the image sensor until the scale line of the micrometer is completely parallel to the screen, and find any two that are far ...
Embodiment 3
[0067] Example three, simulated microscope eyepiece imaging:
[0068] After completing the first, second, and third steps of Example 2, set the under-mirror image display area of the display screen to a diameter of 10 times the number of field of view of the microscope eyepiece (such as 18mm, 20mm, 22mm, 25mm, 26.5 mm) circular display window, select the magnification of the eyepiece and the objective lens or their combined magnification and the number of field of view of the eyepiece on the function button of the screen image display software, and call up the number that is calibrated by the imaging system and stored in the computer The circular display window of the screen image accurately displays the combined magnification and a circular image ten times the diameter of the field of view of the eyepiece. The size and pattern of the circular image are the same as the size of the scene in the physical eyepiece of the same field of view. Same as pattern.
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