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A method for displaying and labeling the scene under the microscope according to the accurate magnification on the screen

A microscope and accurate technology, applied in microscopes, optics, instruments, etc., can solve problems such as labeling, inaccuracy, and irresponsible misleading by observers

Active Publication Date: 2015-11-25
夏潮涌 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although people can use a variable-length straight line segment to represent the actual length value of the corresponding original sample on screens of different sizes and resolutions, no one has yet been able to use specific and accurate numbers to mark the scene under the mirror on the screen How many times the image is the original sample; it is extremely irresponsible to mislead the observer to display the same magnification of the same sample on screens with different sizes and resolutions without calibration of the display system. The magnification value of the scene is not accurate at all
Because such a system only calibrates the imaging system, but does not calibrate the display screens of different sizes and resolutions, and there is no establishment between the imaging system and the screen display system from the original sample size to the microscope. Optical imaging → image sensors with different sizes and resolutions of photosensitive surfaces Electronic imaging → screen display images of different sizes and resolutions The connection between geometric changes in this series of complex magnification change chains, therefore, to explore the calibration methods of imaging systems and screen display systems , and the establishment of an organic connection of geometric changes between the two is the key to realize the accurate marking of any or specified magnification of the screen display image

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1. Ideally restore the color and intensity of the scene under the microscope:

[0054] The first step is to turn the potentiometer control knob of the microscope light source to a large enough size according to the conventional microscopic imaging requirements. After a clear focused scene with suitable brightness is observed in the eyepiece, move the slide on the stage, Make the objective lens and the condenser directly opposite, start the computer imaging software to detect the dynamic brightness of the image displayed on the screen, adjust the light quantity adjustment button of the condenser, and fix the dynamic brightness of the image displayed on the screen at 230 gray values;

[0055] The second step is to turn on the automatic white balance and automatic exposure compensation functions of the image sensor driver software or imaging software. After the blank image displayed on the screen automatically turns gray, turn off the automatic white balance and automati...

Embodiment 2

[0058] Embodiment 2. The integer magnification of the image displayed on the screen is indicated:

[0059] The first step is to calibrate the imaging system: first place the microscope objective micrometer (a special glass slide whose center is divided into 100 equal parts within a distance of 1mm by the scale line) under the observation objective of the microscope, and pass through the eyepiece Observe the image and adjust the focus clearly. At the same time, open the image sensor and imaging system software to display the image on the screen and adjust the focus until the image is clear; turn the image sensor until the scale line of the micrometer is completely perpendicular to the screen, find two at random Enter the scale line on the screen that is far away in the horizontal direction and input its scale information into the imaging system software; turn the image sensor until the scale line of the micrometer is completely parallel to the screen, and find any two that are far ...

Embodiment 3

[0067] Example three, simulated microscope eyepiece imaging:

[0068] After completing the first, second, and third steps of Example 2, set the under-mirror image display area of ​​the display screen to a diameter of 10 times the number of field of view of the microscope eyepiece (such as 18mm, 20mm, 22mm, 25mm, 26.5 mm) circular display window, select the magnification of the eyepiece and the objective lens or their combined magnification and the number of field of view of the eyepiece on the function button of the screen image display software, and call up the number that is calibrated by the imaging system and stored in the computer The circular display window of the screen image accurately displays the combined magnification and a circular image ten times the diameter of the field of view of the eyepiece. The size and pattern of the circular image are the same as the size of the scene in the physical eyepiece of the same field of view. Same as pattern.

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Abstract

The invention belongs to the technical field of displaying and marking vision under a microscope on a screen according to an accurate multiplying factor. By keeping brightness of images displayed on the screen constantly and compensating decayed brightness of a blank imaging area of a carrier, color brilliance and brightness of the vision under the microscope are perfectly displayed by the images displayed on the screen; by demarcating a microscopic imaging system and a screen displaying system, a geometrical relationship is set up between both systems to display the size of an original sample and the accurate multiplying factor of the vision under the microscope in an image displaying area of the screen, and an accurate multiplying factor value and a magnifying scale of a fixed length corresponding to the multiplying factor value are displayed in or out of the displaying area; and the under-microscope image displaying area of the screen is set as a circular displaying area of which the diameter is ten times the number of visual fields of a microscope eyepiece to realize the simulation of the images of the microscope eyepiece.

Description

Technical field [0001] The invention belongs to the field of microscopic imaging and screen display methods, and particularly relates to a method for displaying the screen (including a TV screen, a computer screen and a projection screen) of a scene under a microscope lens and its accurate magnification indication. Background technique [0002] Now, with the development of computer technology and electronic imaging technology, people are no longer satisfied with observing the microscopic scene under the microscope eyepiece with the naked eye. More and more technologies have been used to transfer the scene under the microscope to the TV. Viewing on screens, computer display screens and projection screens, such technologies are collectively referred to as microscopic imaging technologies. In the process of transferring the scene under the microscope to the display screen for viewing, people are very concerned about two issues: 1. The degree of restoration of the scene under the mic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/36
Inventor 夏潮涌夏志坚
Owner 夏潮涌