Application of polysaccharide from mulberry leaves in preventing and treating diabetes
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A technology of mulberry leaf polysaccharide and diabetes, which is applied in the field of natural medicine application, to achieve the effect of simplifying the production process, reducing production cost, and small side effects
Inactive Publication Date: 2010-12-15
HUNAN AGRICULTURAL UNIV
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Therefore, if mulberry leaf polysaccharides have a certain effect on the prevention a
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Embodiment 1
[0014] Embodiment 1: the preparation method of mulberry leaf polysaccharide
[0015] Crush and dry 10kg of mulberry leaves, add 180L of distilled water, heat and reflux at 70°C for 90 minutes, filter twice with three layers of gauze, then filter with a ceramic membrane, concentrate under reduced pressure to 10L, then deproteinize the concentrated solution with 10% trichloroacetic acid, and put it on SEPHADEX G-10 column separation and collection of mulberry leaf polysaccharide fractions, concentration to 1 L, separation and collection of mulberry leaf polysaccharide fractions on a silica gel column, concentration to dryness to obtain mulberry leaf polysaccharides. As determined by the anthrone-concentrated sulfuric acid method, the polysaccharide content of mulberry leaves is 95.5%.
Embodiment 2
[0016] Embodiment 2: Mouse feeding experiment of mulberry leaf polysaccharide
[0017] 1. Diabetic mouse model
[0018] (1) Adult Kunming mice (20-25g) were bred under ventilated conditions (25±1°C), with 12 hours of light and 12 hours of darkness, ad libitum, and standard feeding.
[0019] (2) Induction of diabetic rats: In accordance with the requirement of 45 mg / kg, streptozotocin (dissolved in 0.1M citric acid buffer pH 4.5) was injected intraperitoneally. For 7 consecutive days, blood was collected from the tails of fasting mice, and mice with a fasting blood glucose of 200 mg / dl were selected as experimental subjects. 2. Experimental design:
[0020] 2. Divide the experimental mice into 6 groups, 6 in each group: measure the fasting blood glucose before the experiment
[0021] Group 1: normal non-diabetic rats, drinking distilled water;
[0022] Group 2: Diabetic mice, distilled water;
[0023] Group 3-5: Diabetic rats were given mulberry leaf extract 0.25, 0.5, 1.0...
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Abstract
The invention relates to application of polysaccharide extracted from mulberry leaves in preventing and treating diabetes, belonging to the natural medicine application field. The application method comprises the following steps: crushing 10kg of the dry mulberry leaves, and adding 180L of distilled water to the crushed mulberry leaves; heating and then carrying out reflux extraction on the obtained mixture at the temperature of 70 DEG C for 90min; filtering the obtained extracting solution twice with three-layer gauze and then filtering with a ceramic membrane; decompressing and concentrating the obtained filtrate into 10L, and then deproteinizing the obtained concentrated solution with 10% of trichloroacetic acid; and placing the concentrated solution on an SEPHADEX G-10 column for separating and collecting components containing the polysaccharide from the mulberry leaves, concentrating the solution into 1L, and then placing the concentrated solution on a silicagel column for separating and collecting the components containing the polysaccharide from the mulberry leaves again to obtain the polysaccharide from the mulberry leaves, wherein, content of the polysaccharide from the mulberry leaves is 95.5%.
Description
Technical field: [0001] The invention relates to the application of mulberry leaf polysaccharide extracted from mulberry leaves in the prevention and treatment of diabetes, which belongs to the technical field of natural medicine application. Background technique [0002] So far, there are 130 million diabetic patients in the world, and the number is still increasing rapidly. Diabetes has become the third killer after cardiovascular and tumors, and is one of the three major public health problems in the world. According to data released by the Ministry of Health, in 2009 there were more than 41 million diabetic patients in urban areas alone, with an average of 1.5 million new patients each year. Diabetes is a common disease caused by the interaction of genetic and environmental factors. Clinically, hyperglycemia is the main sign, and common symptoms include polydipsia, polyuria, polyphagia, and weight loss. Diabetes can cause damage to multiple systems in the body, cause a...
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