I-type canine adenovirus attenuated vaccine strain and application thereof

A canine adenovirus and attenuated vaccine technology, applied in antiviral agents, inactivation/attenuation, nervous system diseases, etc., can solve the problems of high cost and poor antigen targeting, and achieve good protection and good immune protection effect, good immunogenicity

Inactive Publication Date: 2010-12-15
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the above vaccines have disadvantages such as high cost and po

Method used

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  • I-type canine adenovirus attenuated vaccine strain and application thereof
  • I-type canine adenovirus attenuated vaccine strain and application thereof
  • I-type canine adenovirus attenuated vaccine strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1I

[0014] Isolation and identification of embodiment 1 type I canine adenovirus (CAV-H) isolate

[0015] 1 Materials and methods

[0016] 1.1 Disease material: The affected dog came from Heilongjiang Province. The clinical manifestations were elevated body temperature, watery eyes and nose, and bloody stool. The sick dog died within 3 days of the illness. Intestinal contents were collected for virus isolation.

[0017] 1.2 Cells: MDCK (generation 74-94, the same below) cell line was purchased from China Veterinary Drug Control Institute.

[0018] 1.3 Experimental animals: 2-4 month-old puppies (CAV antibody titer ≤ 1:2) were selected and provided by the Department of Experimental Zoology of Harbin Medical University. After deworming and 1w observation, it was determined that the healthy ones were available.

[0019] 1.4 Serum: canine anti-CAV (SN≥1:16) standard positive serum and negative serum were made by the inventor's laboratory.

[0020] 1.5 Isolation of virus: Take the i...

Embodiment 2I

[0079] The cultivation of embodiment 2 type I canine adenovirus attenuated vaccine strain CAV-HR

[0080] 1 Materials and methods

[0081] 1.1 Test materials The test animals are healthy dogs aged 2 to 4 months (CAV antibody titer ≤ 1:2); anti-canine adenovirus-specific serum and 1% red blood cell suspension are prepared by our laboratory, and the strong toxicity is CAV-H 6 generation.

[0082] 1.2 Cultivation and identification of CAV-H attenuated strain

[0083] 1.2.1 Cultivation of attenuated CAV-H strain

[0084] CAV-H was passaged through MDCK cells to passage 110, during which clonal purification was carried out 3 times.

[0085] 1.2.2 Morphological observation of attenuated CAV-H strain

[0086] Centrifuge the harvested CAV-H 5, 10, 20, 30, 50, 70, 90, and 110 passage virus cell cultures at 5000r / min for 30min, take the supernatant and ultracentrifuge at 20000r / min, centrifuge at 4°C for 2h, and use for precipitation Appropriate amount of PBS (pH 7.0) suspended, ph...

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Abstract

The invention discloses an I-type canine adenovirus attenuated vaccine strain and application thereof. In the invention, the separated I-type canine adenovirus attenuated vaccine strain is carried out passage and cloned on MDCK (Madin-Darby Canine Kidney), and attenuated strains (CAV-HR) are cultured. The security and immunogenicity test result shows that the attenuated strains CAV-HR can provide good protection force for dogs attacked by isogeny virulent virus. The attenuated strains (CAV-HR) has favorable immunogenicity, can be prepared into a single seed or combined seeds, and can effectively prevent or treat the diseases caused by the I-type canine adenovirus. The attenuated vaccine strain has the advantages of stable hereditary, lasting immunity, good effect, security, reliability, long preservation period and the like.

Description

technical field [0001] The present invention relates to a strain of virus attenuated vaccine strain, especially relate to an attenuated vaccine strain CAV-HR weakened by type I canine adenovirus virulent strain CAV-H, the present invention also relates to this attenuated vaccine strain in the prevention or treatment of The application in various diseases caused by type I canine adenovirus belongs to the field of prevention or treatment of type I canine adenovirus. Background technique [0002] Canine adenovirus type I was first discovered in 1925. In 1984, the virus was isolated for the first time in my country, which confirmed the infection of canine adenovirus type I in dogs in my country (Yin Zhen, Liu Jinghua. Animal Virology (Second Edition). Beijing: Science Press, 1997, 757-762 .). Type I canine adenovirus can clinically cause canine infectious hepatitis (Webe J.DNA vaccine.Business Week, 1996, 2:6.), fox and bear encephalitis (Xia Chengzhu, Fan Quanshui, Hu Rongliang...

Claims

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Application Information

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IPC IPC(8): C12N7/08A61K39/235A61P31/20A61P1/16A61P25/00C12R1/93
Inventor 刘大飞张洪英曲联东王牟平刘立奎
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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